Molecular identification methods of fish species: reassessment and possible applications

2009 ◽  
Vol 19 (3) ◽  
pp. 265-293 ◽  
Author(s):  
Fabrice Teletchea
2021 ◽  
Vol 78 (6) ◽  
pp. 373-380
Author(s):  
Oleh Prylutskyi ◽  
◽  
Oleksandr Zinenko ◽  
Polina Havrysh ◽  
◽  
...  

New records of four species of the genus Lepiota (Agaricales, Basidiomycota) are reported from Ukraine. Three species, L. fuscovinacea, L. griseovirens, and L. roseolivida, are recorded in Ukraine for the first time, whereas a poorly known species, L. subalba, earlier known in Ukraine from a few records, is confirmed using molecular identification methods. All species reports are supplemented with original descriptions and drawings based on newly collected material, as well as data on general distribution, habitat, references to new collections and public databases. Original nucleotide sequence of the ITS region of ribosomal DNA obtained from our voucher specimen of L. subalba is provided.


2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Alexandre dos Santos Rodrigues ◽  
José Henrique Souza Galdino Brandão ◽  
Jamille de Araújo Bitencourt ◽  
Ricardo Jucá-Chagas ◽  
Iracilda Sampaio ◽  
...  

Lignobrycon myersiis a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species,L. myersiis occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset forL. myersi, we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species (Triportheusspp.) and other fish species (Astyanax fasciatus) commonly mixed withL. myersiin street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminatingL. myersispecimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading ofL. myersiand to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.


2018 ◽  
Vol 7 (3) ◽  
pp. 38-50 ◽  
Author(s):  
NAYANA GUNATHILAKA

Mosquito borne diseases remains as an importance source of morbidity and mortality in Sri Lanka. To better control vectors which transmit the diseases, updated list of the species present in the county is imperative. It is also vital in documenting the diversity of the family Culicidae. Original records were collected from a literature review to compile a list of the species recorded in Sri Lanka. This work illustrates the updated list of mosquito species in Sri Lanka and their current taxonomic status based on previous studies from 1901 to date. A total of 159 species belonging to 19 genera including sibling species, have been included in the revised mosquito checklist in Sri Lanka. The present work includes 13 species, two genera (Lutzia, Verrallina) and 9 subgenera in subfamily Culicinae, tribe Aedini of genus Aedes (Bruceharrisonius, Collessius, Danielsia, Dendroskusea, Downsiomyia, Fredwardsius, Hulecoeteomyia, Neomelaniconion, Phagomyia) in to the checklist which were not included in the previous mosquito checklist published nearly 26 years ago. However, further work is essential to refine this list and to explore the abundance of new species within the country. Improved morphological and molecular identification methods will sanction the refinement of the mosquito catalog in years to come.


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