scholarly journals Molecular Identification and Traceability of Illegal Trading inLignobrycon myersi(Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Alexandre dos Santos Rodrigues ◽  
José Henrique Souza Galdino Brandão ◽  
Jamille de Araújo Bitencourt ◽  
Ricardo Jucá-Chagas ◽  
Iracilda Sampaio ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Fish Species ◽  
Human Impacts ◽  
Local Population ◽  
Dna Barcode ◽  
Northeastern Brazil ◽  
Genetic Stocks ◽  
Population Structuring ◽  
Fresh Frozen ◽  
Coi Sequences

Lignobrycon myersiis a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species,L. myersiis occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset forL. myersi, we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species (Triportheusspp.) and other fish species (Astyanax fasciatus) commonly mixed withL. myersiin street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminatingL. myersispecimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading ofL. myersiand to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.

Research Paper DNA barcode identification of fish products from Guiyang markets in southwest China

2022 ◽  
Author(s):  
Qian Tang ◽  
Qi Luo ◽  
Qian Duan ◽  
Lei Deng ◽  
Renyi Zhang
Keyword(s):  
Dna Barcoding ◽  
Molecular Identification ◽  
Fish Consumption ◽  
Southwest China ◽  
Dna Barcode ◽  
Guizhou Province ◽  
Accurate Identification ◽  
Continuous Growth ◽  
Fish Products ◽  
Fresh Frozen

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.

DNA barcode-based molecular identification system for fish species

2010 ◽  
Vol 30 (6) ◽  
pp. 507-512 ◽  
Author(s):  
Sungmin Kim ◽  
Hae-Seok Eo ◽  
Hyeyoung Koo ◽  
Jun-Kil Choi ◽  
Won Kim
Keyword(s):  
Molecular Identification ◽  
Fish Species ◽  
Dna Barcode ◽  
Identification System

scholarly journals Linking adults and immatures of South African marine fishes

Genome ◽  
2016 ◽  
Vol 59 (11) ◽  
pp. 959-967 ◽  
Author(s):  
Dirk Steinke ◽  
Allan D. Connell ◽  
Paul D.N. Hebert
Keyword(s):  
South African ◽  
Fish Species ◽  
Dna Barcode ◽  
Early Life History ◽  
Significant Advance ◽  
Reference Library ◽  
Larval Recruitment ◽  
Fisheries Science ◽  
Class Strength ◽  
Coi Sequences

The early life-history stages of fishes are poorly known, impeding acquisition of the identifications needed to monitor larval recruitment and year-class strength. A comprehensive database of COI sequences, linked to authoritatively identified voucher specimens, promises to change this situation, representing a significant advance for fisheries science. Barcode records were obtained from 2526 early larvae and pelagic eggs of fishes collected on the inshore shelf within 5 km of the KwaZulu-Natal coast, about 50 km south of Durban, South Africa. Barcodes were also obtained from 3215 adults, representing 946 South African fish species. Using the COI reference library on BOLD based on adults, 89% of the immature fishes could be identified to a species level; they represented 450 species. Most of the uncertain sequences could be assigned to a genus, family, or order; only 92 specimens (4%) were unassigned. Accumulation curves based on inference of phylogenetic diversity indicate near-completeness of the collecting effort. The entire set of adult and larval fishes included 1006 species, representing 43% of all fish species known from South African waters. However, this total included 189 species not previously recorded from this region. The fact that almost 90% of the immatures gained a species identification demonstrates the power and completeness of the DNA barcode reference library for fishes generated during the 10 years of FishBOL.

scholarly journals New records of the occurrence of Megaleporinus macrocephalus () (Characiformes, Anostomidae) from the basins of the Itapecuru and Mearim rivers in Maranhão, Northeastern Brazil

2022 ◽  
Vol 82 ◽  
Author(s):  
M. S. Almeida ◽  
P. S. S. Moraes ◽  
M. H. S. Nascimento ◽  
J. L. O. Birindelli ◽  
F. M. Assega ◽  
...  
Keyword(s):  
Fish Species ◽  
New Records ◽  
Northeastern Brazil ◽  
Coi Gene ◽  
Fish Farms ◽  
Exotic Fish ◽  
Paraguay River ◽  
Coi Sequences ◽  
Brazilian Northeast ◽  
Fish Tanks

Abstract The “piaussu”, Megaleporinus macrocephalus is an anostomatid fish species native to the basin of the Paraguay River, in the Pantanal biome of western Brazil. However, this species has now been recorded in a number of other drainages, including those of the upper Paraná, Uruguay, Jacuí, Doce, Mucuri, and Paraíba do Sulrivers. This study presents two new records of the occurrence of M. macrocephalus, in the basins of the Itapecuru and Mearim rivers in the state of Maranhão, in the Brazilian Northeast. The piaussu is a large-bodied fish of commercial interest that is widely raised on fish farms, and its occurrence in the Itapecuru and Mearim rivers is likely the result of individuals escaping from fish tanks when they overflow during the rainy season. Morphological analyses and sequences of the Cytochrome Oxidase Subunit I (COI) gene confirmed the taxonomic identification of the specimens as M. macrocephalus. The COI sequences were 99.66% similar to those of M. macrocephalus deposited in the BOLDSystems database. These records extend the known distribution of M. macrocephalus to the basins of the Itapecuru and Mearim rivers in the Brazilian Northeast, highlighting a new case of introduction of exotic fish species into Brazilian river basins.

scholarly journals Analysis of reproductive biology and spawning season of the pink ear emperor Lethrinus lentjan, from marine ecosystem

2020 ◽  
Vol 37 ◽  
pp. 1-10
Author(s):  
Elsayed M. Younis ◽  
Nasser A. Al-Asgah ◽  
Abdel-Wahab A. Abdel-Warith ◽  
Mohamed H. Gabr ◽  
Fozi S. Shamlol
Keyword(s):  
Fish Species ◽  
Gonadosomatic Index ◽  
Marine Ecosystem ◽  
Local Population ◽  
Spawning Season ◽  
Monthly Basis ◽  
Population Sampling ◽  
Maturation Rate ◽  
Maturation Index ◽  
One Year

A total of 593 samples of Lethrinus lentjan (Lacepede, 1802) were collected from the Red Sea, Jeddah, Saudi Arabia, to study their productive biology and spawning season of the local population. Sampling was carried out on a monthly basis for a period of one year. The monthly sex ratios indicated that females were dominant throughout the study period, with an overall male:female sex ratio of 1:7.98, although males were larger than females. The highest monthly performance maturation index (PMI), as well as the male and female gonadosomatic index (GSI) and ovarian maturation rate (OMR) were observed in February and March. Histological examination of the gonads confirmed the process of sexual transformation in this fish species, wherein individuals mature first as female, and then change sex to male (protogynous hermaphroditism). Histological sections also showed that the sexual maturation of males of L. lenjtan comprised three main stages, while the sexual development of females could be classified into four main stages. Extended spawning in the form of batches released during different months throughout the year were recorded for this fish species, with the main spawning season in February and March, and an additional, shorter spawning season in September.

scholarly journals DNA barcodes and morphology of P. krempfi, P. mekongensis, and P. elongatus

2016 ◽  
Vol 14 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Dương Thúy Yên ◽  
Nguyễn Kiệt ◽  
Bùi Sơn Nên ◽  
Nguyễn Văn Thường ◽  
Nguyễn Bạch Loan ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Mekong Delta ◽  
Genetic Distances ◽  
Dna Barcodes ◽  
Gill Rakers ◽  
Coi Sequences ◽  
Relationship Of ◽  
Metric Traits ◽  
I Gene

Three Pangasius species including P. krempfi, P. elongatus and P. mekongensis, are economically important. They can be mis-identified due to similar external appreance at small sizes. This study aimed to distinguish these species based on their differences in DNA barcode, COI (cytochrome c oxidase subunit I) gene, and morphological characteristics. Fish with various sizes (>90 samples/species) were sampled at the lower Mekong delta region. Kimura-2 parameter genetic distances based on COI sequences of three species (15 samples, in which, 4 unique sequences were assigned Genbank accession numbers from KT289877 to KT289880) are relatively high, ranging 9.33 – 12.10 %. Morphological measurements show that coutanble traits including numbers of fin rays and the first gill rakers vary in similar ranges but ratios of metric traits are significantly different among three species (P<0.01). Principle component analysis using metric traits sets three species apart. P. elongatus is characterized by elongated body, long caudal preduncle, large eyes, and retangle palatine tooth plates. P. krempfi differs from P. mekongesis in characteristics on their head. The number of sections, shape and length of barbel are different among three species. Phylogenetic relationship of three species based on morphology and COI sequences indicate that P. krempfi is closer to P. mekongenis rather than P. elongatus, and that the distance between P. mekongenis and P. elongatus is the largest.

scholarly journals A new species of Litarachna Walter, 1925 (Acari: Hydrachnidia: Pontarachnidae) from Corozal Bay (Belize), described based upon morphology and DNA barcodes

Acarologia ◽  
2021 ◽  
Vol 61 (3) ◽  
pp. 602-613
Author(s):  
Lucia Montes-Ortiz ◽  
Tom Goldschmidt ◽  
Lourdes Vásquez-Yeomans ◽  
Manuel Elías-Gutiérrez
Keyword(s):  
Dna Barcode ◽  
Morphological Description ◽  
Estuarine System ◽  
Dna Barcodes ◽  
Sem Images ◽  
The Family ◽  
Coi Sequences ◽  
First Time ◽  
A New Species ◽  
Electronic Microscope

A new planktonic species of the marine water mites of the family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and is augmented by an analysis of DNA cytochrome c oxidase I (COI) sequences, the DNA barcode, used for the first time for a species description in this group.

scholarly journals debar, a sequence-by-sequence denoiser for COI-5P DNA barcode data

2021 ◽  
Author(s):  
Cameron M. Nugent ◽  
Tyler A. Elliott ◽  
Sujeevan Ratnasingham ◽  
Paul D. N. Hebert ◽  
Sarah J. Adamowicz
Keyword(s):  
High Throughput Sequencing ◽  
Dna Barcode ◽  
R Package ◽  
Error Rates ◽  
Real World Data ◽  
Species Discovery ◽  
Consensus Sequences ◽  
In Silico Studies ◽  
Coi Sequences

AbstractDNA barcoding and metabarcoding are now widely used to advance species discovery and biodiversity assessments. High-throughput sequencing (HTS) has expanded the volume and scope of these analyses, but elevated error rates introduce noise into sequence records that can inflate estimates of biodiversity. Denoising —the separation of biological signal from instrument (technical) noise—of barcode and metabarcode data currently employs abundance-based methods which do not capitalize on the highly conserved structure of the cytochrome c oxidase subunit I (COI) region employed as the animal barcode. This manuscript introduces debar, an R package that utilizes a profile hidden Markov model to denoise indel errors in COI sequences introduced by instrument error. In silico studies demonstrated that debar recognized 95% of artificially introduced indels in COI sequences. When applied to real-world data, debar reduced indel errors in circular consensus sequences obtained with the Sequel platform by 75%, and those generated on the Ion Torrent S5 by 94%. The false correction rate was less than 0.1%, indicating that debar is receptive to the majority of true COI variation in the animal kingdom. In conclusion, the debar package improves DNA barcode and metabarcode workflows by aiding the generation of more accurate sequences aiding the characterization of species diversity.

Identification of larvae of two Aphodius Helwig, 1798 (Coleoptera: Scarabaeidae: Aphodiinae) species using morphology and DNA barcode

Zootaxa ◽  
2021 ◽  
Vol 5047 (2) ◽  
pp. 153-164
Author(s):  
ANDREY V. FROLOV ◽  
MARIA S. VISHNEVSKAYA ◽  
LILIA A. AKHMETOVA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dna Barcode ◽  
The Third ◽  
Coi Sequences ◽  
First Time ◽  
Scanning Electron ◽  
Third Instar Larvae

The third instar larvae of Aphodius (Alocoderus) hydrochaeris (Fabricius, 1798) and A. (Bodilus) ictericus (Laicharting, 1781) are described based on scanning electron microscopy and COI sequences. COI barcode sequence for A. (A.) hydrohaeris is provided for the first time. Two haplotypes are discovered in A. (B.) ictericus.  

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