Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F.

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words: Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v25i1.24110 ◽

2015 ◽

Vol 25 (1) ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Kee Hwa Bae ◽

Eui Soo Yoon

Keyword(s):

Plant Regeneration ◽

Seed Germination ◽

Callus Induction ◽

In Vitro Propagation ◽

Ornamental Plants ◽

Leaf Explants ◽

Adventitious Shoot ◽

Temperature Treatment ◽

In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.527 ◽

2011 ◽

Vol 10 (51) ◽

pp. 10509-10515 ◽

Cited By ~ 1

Author(s):

H Zhang Y ◽

F Gao S ◽

Du T ◽

G Chen H ◽

H Z Wang ◽

...

Keyword(s):

Plant Regeneration ◽

Multiple Shoot ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Codonopsis Pilosula

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In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v21i2.10236 ◽

2011 ◽

Vol 21 (2) ◽

pp. 135-141 ◽

Cited By ~ 2

Author(s):

A.K. M. Sayeed Hassan ◽

Nadira Begum ◽

Rebeka Sultana ◽

Rahima Khatun

Keyword(s):

Survival Rate ◽

Plant Regeneration ◽

Room Temperature ◽

Shoot Proliferation ◽

Nodal Explants ◽

Shoot Induction ◽

Half Strength ◽

Regenerated Plantlets ◽

In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration D. O. I. 10.3329/ptcb.v21i2.10236 Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

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Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance ofAjuga multifloraBunge by Altering Activity of Antioxidant Enzyme

The Scientific World JOURNAL ◽

10.1155/2014/521703 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 31

Author(s):

Iyyakkannu Sivanesan ◽

Byoung Ryong Jeong

Keyword(s):

Salinity Tolerance ◽

Shoot Regeneration ◽

Microscopic Analysis ◽

Adventitious Shoot ◽

Induction Medium ◽

Shoot Induction ◽

Electron Microscopic ◽

Sod Activity ◽

Scanning Electron Microscopic Analysis ◽

Shoot Induction Medium

We investigated the effect of Si concentration on shoot regeneration and salinity tolerance ofAjuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium containing NaCl alone, while there was less decrease when the shoot induction medium was supplemented with both NaCl and Si. The shoot induction percentage increased linearly with increasing concentration of Si in the NaCl containing medium. Addition of Si to the shoot induction medium significantly increased SOD, POD, APX, and CAT activity in regenerated shoot buds as compared with the control. The inclusion of Si to the NaCl containing medium significantly increased the SOD activity in leaves and roots, while it decreased POD, APX, and CAT activity in both organs. Scanning electron microscopic analysis showed that there are no distinct differences in the structure of stomata between the control and Si-treated plants. However, NaCl treatment significantly affected the structure and number of stomata as compared to the control. Wavelength dispersive X-ray analysis confirmed the high Si deposition in trichomes of plants grown in the Si containing medium but not in plants grown in the medium without Si.

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