Molecular characterization of a novel AP2 transcription factor ThWIND1-L from Thellungiella halophila

2012 ◽  
Vol 110 (3) ◽  
pp. 423-433 ◽  
Author(s):  
Cheng Zhou ◽  
Jiansheng Guo ◽  
Zhenhua Feng ◽  
Xianghuan Cui ◽  
Jian Zhu
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Thellungiella Halophila ◽  
Ap2 Transcription Factor

scholarly journals Isolation and molecular characterization of a Lotus japonicus R2R3-MYB subgroup 7 transcription factor gene

2017 ◽  
Vol 34 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Satoru Kunihiro ◽  
Daigo Tanabe ◽  
Yuiko Niwa ◽  
Keisuke Kitamura ◽  
Jun Abe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Lotus Japonicus ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
R2r3 Myb

scholarly journals Molecular Characterization of Saccharomyces cerevisiae TFIID

2002 ◽  
Vol 22 (16) ◽  
pp. 6000-6013 ◽  
Author(s):  
Steven L. Sanders ◽  
Krassimira A. Garbett ◽  
P. Anthony Weil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Molecular Mass ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Gel Filtration ◽  
Calculated Molecular Mass ◽  
General Transcription Factor

ABSTRACT We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription in vitro and displays TATA box binding activity that is functionally distinct from that of TBP. Analyses of the stoichiometry of TFIID subunits indicated that several TAFs are present at more than 1 copy per TFIID complex. This conclusion was further supported by coimmunoprecipitation experiments with a systematic family of (pseudo)diploid yeast strains that expressed epitope-tagged and untagged alleles of the genes encoding TFIID subunits. Based on these data, we calculated a native molecular mass for monomeric TFIID. Purified TFIID behaved in a fashion consistent with this calculated molecular mass in both gel filtration and rate-zonal sedimentation experiments. Quite surprisingly, although the TAF subunits of TFIID cofractionated as a single complex, TBP did not comigrate with the TAFs during either gel filtration chromatography or rate-zonal sedimentation, suggesting that TBP has the ability to dynamically associate with the TFIID TAFs. The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae.

Molecular characterization of Quercus suber MYB1, a transcription factor up-regulated in cork tissues

2013 ◽  
Vol 170 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Tânia Almeida ◽  
Esther Menéndez ◽  
Tiago Capote ◽  
Teresa Ribeiro ◽  
Conceição Santos ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Quercus Suber

scholarly journals A GABAergic Maf-expressing interneuron subset regulates the speed of locomotion in Drosophila

2018 ◽  
Author(s):  
H Babski ◽  
C Surel ◽  
S Yoshikawa ◽  
J Valmier ◽  
J.B Thomas ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Muscle Contractions ◽  
Body Posture ◽  
Molecular Signature ◽  
Small Subset ◽  
Larval Body ◽  
Traffic Jam ◽  
Locomotion Speed

AbstractInterneurons (INs) coordinate motoneurons activity to generate adequate patterns of muscle contractions, providing animals with the ability to adjust their body posture and to move over a range of speeds. In the Drosophila larvae several IN subtypes have been morphologically described and their function well documented. However, the general lack of molecular characterization of those INs prevents the identification of evolutionary counterparts in other model animals, limiting our understanding of widespread principles ruling neuronal circuits organization and functioning. Here we characterize a highly restricted neuronal subset expressing the Maf transcription factor Traffic Jam (TJ). We found that TJ+ neurons are highly diverse and their activation using intersectional genetics disrupted larval body posture and locomotion speed. We also showed that a small subset of TJ+ GABAergic INs, singled out by the unique expression of Per, Fkh, Grain and Hlh3b, a molecular signature reminiscent to V2b INs in vertebrate, impacted the larvae crawling speed.

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