A novel co-culture system of adventitious roots of Echinacea species in bioreactors for high production of bioactive compounds

2017 ◽  
Vol 130 (2) ◽  
pp. 301-311 ◽  
Author(s):  
C. H. Wu ◽  
D. An ◽  
L. N. Sun ◽  
M. Wang ◽  
G. N. Chang ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Culture System ◽  
Adventitious Roots ◽  
High Production

scholarly journals MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones

2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Bartosz Nowak ◽  
Mateusz Kawka ◽  
Kamil Wierzchowski ◽  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek
Keyword(s):  
Polyurethane Foam ◽  
Bioactive Compounds ◽  
Hairy Roots ◽  
Reference System ◽  
Culture System ◽  
In Vitro Cultures ◽  
Fresh Mass ◽  
Extracellular Secretion ◽  
High Level

Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of Rindera graeca hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. R. graeca hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward R. graeca hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg gDW−1, was noted for PUF-supported culture system.

An In Vitro Culture System for the Conservation and Production of Bioactive Compounds from Lilium dauricum

2019 ◽  
Vol 27 (4) ◽  
pp. 285-295
Author(s):  
Jin-Ho Kim ◽  
◽  
Thanh-Tam Ho ◽  
Eun Bi Jang ◽  
Seolah Kim ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Culture System

Production of biomass and bioactive compounds from shoot cultures of Rosa rugosa using a bioreactor culture system

2016 ◽  
Vol 57 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Hae-Rim Jang ◽  
Hyun-Jeong Lee ◽  
A. M. Shohael ◽  
Byung-Jun Park ◽  
Kee-Yoeup Paek ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Culture System ◽  
Bioreactor Culture ◽  
Shoot Cultures ◽  
Rosa Rugosa

High production of β-thujaplicin with Thuja dolabrata var. hondai cells in a semi-continuous culture system

2000 ◽  
Vol 11 (1) ◽  
pp. 59-61 ◽  
Author(s):  
Kazumi Sanada ◽  
Akihiko Kawaguchi ◽  
Tsutomu Furuya ◽  
Kohji Ishihara ◽  
Nobuyoshi Nakajima ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Continuous Culture System ◽  
High Production

scholarly journals Rhizogenesis in Cell Suspension Culture from Mango Ginger: A Source of Isosorbide and n-Hexadecanoic Acid

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Chellappan Soundar Raju ◽  
Kandhan Varutharaju ◽  
Chandrasekaran Thilip ◽  
Abubakker Aslam ◽  
Appakan Shajahan
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Bioactive Compounds ◽  
Cell Suspension Culture ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Production ◽  
Hexadecanoic Acid ◽  
Mango Ginger ◽  
Initial Inoculum

Curcuma amada Roxb. belongs to the monocotyledonous family Zingiberaceae. It is commonly known as mango ginger and used as a spice and valuable medicine. In this study, adventitious roots of C. amada have been successfully established from cell suspension culture. The highest percentage of adventitious root production was obtained from friable callus derived cell suspension culture. The culture conditions of adventitious root were optimized and the maximum adventitious root production was obtained in half strength MS liquid medium containing 0.3 mg L−1 IBA along with 3% of sucrose after 5 weeks of culture. Among the different initial inoculum density, the best culture condition for root growth occurred at 10 g FW of initial inoculum density. GC-MS analysis revealed that the in vitro raised adventitious roots containing two valuable bioactive compounds, isosorbide and n-hexadecanoic acid. The outcome of the present work will be helpful for the large scale cultivation of adventitious roots for the production of valuable bioactive compounds.

A culture system for the stable and high-efficiency proliferation of adventitious roots of Panax notoginseng and ginsenoside accumulation

2020 ◽  
Vol 157 ◽  
pp. 112882 ◽  
Author(s):  
Yue Zhao ◽  
Wen-Hua Guo ◽  
Xiao-Yang Sun ◽  
Kui-Hua Li ◽  
Ke-Jian Liu ◽  
...  
Keyword(s):  
Culture System ◽  
Adventitious Roots ◽  
High Efficiency ◽  
Panax Notoginseng

scholarly journals Bioprospecting of an Indigeneous Bacillus thuringiensis Strain G5-8-3T02 for Shrimp Culture System

2020 ◽  
pp. 33-43
Author(s):  
Caroline Nchedo Ariole ◽  
Euphemia Uchechi Agulanna
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Thuringiensis ◽  
Bioactive Compounds ◽  
Culture System ◽  
Sodium Dodecyl ◽  
Quantitative Composition ◽  
Potential Candidate ◽  
Shrimp Culture ◽  
Vibrio Mimicus ◽  
Thuringiensis Strain

Aim: An indigenous Bacillus thuringiensis strain G5-8-3T02 earlier isolated from healthy shrimp intestine and established to safeguard Penaeus monodon from Vibrio mimicus infection was assessed as a potential candidate bacterium for drug enhancement for shrimp culture systems. Methodology: Optimal temperature, pH and salinity for growth and antibacterial activity of Bacillus thuringiensis against Vibrio mimicus were determined. The effects of enzymes (pepsin, catalase, lipase and α- amylase), pH (2-9), heat (37°C – 121°C) and surfactants (Sodium dodecyl sulphate and Tween 80) on antibacterial activity were also evaluated. Cell growth and antibacterial activity were monitored daily for 5 days via spectrophotometric method at 600 nm and agar well diffusion assay respectively. The metabolite extracted with methanol was analyzed for bioactive compounds using Gas Chromatography-Mass Spectrometry (GC-MS). Results: The optimum temperature, pH and salinity for growth and metabolite production were 35°C, pH 6-7 and 10-15 ppt. respectively. The crude extract did not totally lose its activity when treated with enzymes, various pH values, heat and surfactants. The quantitative composition of the metabolite revealed 15 bioactive compounds. The main compound produced was 2, 6, 10-Trimethyltridecane with peak area of 14.58%. Conclusion: The non-pathogenic, antagonistic, indigenous Bacillus thuringiensis strain G5-8-3T02 could be engaged in the management of vibriosis in shrimp culture since the use of antibiotics in aquaculture for disease control yields objectionable effects. The identified bioactive compounds might be valuable for drug development for shrimp culture system.

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