Micropropagation of Bambusa oldhamii Munro in heterotrophic, mixotrophic and photomixotrophic systems

2020 ◽  
Vol 141 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Andreia Alves da Costa Silveira ◽  
Francis Julio Fagundes Lopes ◽  
Sérgio Tadeu Sibov
Keyword(s):  
Bambusa Oldhamii

SISTEMAS DE PRODUCCIÓN DE BAMBÚ (Guadua angustifolia Kunth y Bambusa oldhamii Munro) EN LA SIERRA NORORIENTAL DE PUEBLA, MÉXICO

2018 ◽  
Vol 11 (8) ◽  
Author(s):  
B. Ramírez-Valverde
Keyword(s):  
El Sistema ◽  
Bambusa Oldhamii

La utilización del bambú es muy antigua, pero poco conocida en México. En Puebla, los productores están incorporando bambúes a sus predios como parte de la diversificación. El objetivo del estudio es conocer los sistemas de producción de Guadua angustifolia Kunth, y Bambusa oldhamii Munro y describir su aprovechamiento por los pobladores en tres comunidades de la Sierra Nor-Oriental de Puebla, México. Se aplicaron encuestas a pequeños productores y a productores comerciales de bambú de la zona. Se encontraron dos grandes sistemas de bambú, el sistema de plantaciones comerciales, mayor a media hectárea exclusiva para bambú, mantenimiento de la plantación y producción destinada al mercado en distintas formas. El sistema familiar está presente en la mayoría de los productores, el bambú se encuentra en los pequeños predios de cultivo, principalmente maíz y frijol, no existe manejo agronómico y los usos son exclusivos para las necesidades del hogar. Las condiciones climáticas presentes en la Sierra Nor Oriental, benefician el desarrollo del cultivo de bambú, sin embargo, el principal problema del cultivo en ambos sistemas, es la falta de mercado, el poco comercio se da de forma incipiente y descuidada, por tal motivo han buscado diversificar sus fincas sin éxito, ya que no existe una cadena productiva de bambú en la zona.

scholarly journals The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dan Hou ◽  
Ling Li ◽  
Tengfei Ma ◽  
Jialong Pei ◽  
Zhongyu Zhao ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Large Scale ◽  
Flowering Plants ◽  
Activation Functions ◽  
Regulatory Activity ◽  
Mature Leaves ◽  
Sporadic Flowering ◽  
Bamboo Flowering ◽  
Bambusa Oldhamii

AbstractBamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii.

Cloning and characterization of the Bambusa oldhamii BoMDH-encoded malate dehydrogenase

2020 ◽  
Vol 174 ◽  
pp. 105665
Author(s):  
Che-Jen Hsiao ◽  
Chun-Yen Hsieh ◽  
Lu-Sheng Hsieh
Keyword(s):  
Malate Dehydrogenase ◽  
Bambusa Oldhamii

scholarly journals In vitro establishment of Bambusa oldhamii Munro from field-grown matrices and molecular identification of endophytic bactéria

2019 ◽  
Vol 49 ◽  
Author(s):  
Ana Paula de Azevedo Pasqualini ◽  
Gabriela Xavier Schneider ◽  
Hugo Pacheco de Freitas Fraga ◽  
Luiz Antonio Biasi ◽  
Marguerite Quoirin
Keyword(s):  
Molecular Identification ◽  
Culture Medium ◽  
Bacterial Isolate ◽  
Fungal Growth ◽  
Endophytic Bacterium ◽  
Liquid Culture Medium ◽  
Rdna Sequencing ◽  
In Vitro Establishment ◽  
Bambusa Oldhamii

ABSTRACT In plant micropropagation, the establishment stage is difficult, due to the presence of microorganisms in tissues from field-grown matrices, especially for bamboo. This study aimed to establish an efficient asepsis protocol for Bambusa oldhamii explants from field plants, as well as to carry out the molecular identification of a possible endophytic bacterial isolate. The explants were exposed to 70 % alcohol, 1 % sodium hypochlorite (NaOCl), 0.1 % mercuric chloride (HgCl2), thiophanate-methyl (Cercobin®) and chlorhexidine digluconate (2 % Riohex®) in different combinations, and introduced into Murashige and Skoog culture medium (solid or liquid), supplemented or not with 4 mL L-1 of Plant Preservative Mixture (PPMTM), totaling seven treatments. The asepsis and immersion of the explants in the liquid culture medium containing 4 mL L-1 of PPMTM visually inhibited the bacterial and fungal growth, allowed the development of shoots with a mean length of 2.2 cm and posterior subcultures, being the best treatment used for the in vitro establishment of B. oldhamii. The molecular identification of an endophytic bacterium performed by 16S rDNA sequencing allowed to identify the bacterial isolate as Ralstonia sp., with 100 % of similarity, and the phylogenetic analysis grouped it with Ralstonia pickettii. In addition, the bacterial isolate showed to be sensitive to 4 mL L-1 of PPMTM by the minimum inhibitory concentration test.

scholarly journals Isolation, identification and characterization of endophytic fungi of Bambusa oldhamii munro applied as antagonists to Pyricularia oryzae

Revista CERES ◽  
2020 ◽  
Vol 67 (4) ◽  
pp. 296-305
Author(s):  
Andreia Alves da Costa Silveira ◽  
Leila Garcês Araújo ◽  
Marta Cristina Corsi de Fillipi ◽  
Sérgio Tadeu Sibov
Keyword(s):  
Endophytic Fungi ◽  
Pyricularia Oryzae ◽  
Identification And Characterization ◽  
Bambusa Oldhamii

scholarly journals Phenylalanine, Tyrosine, and DOPA Are Bona Fide Substrates for Bambusa oldhamii BoPAL4

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1263
Author(s):  
Chun-Yen Hsieh ◽  
Yi-Hao Huang ◽  
Hui-Hsuan Yeh ◽  
Pei-Yu Hong ◽  
Che-Jen Hsiao ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cinnamic Acid ◽  
Phenylalanine Ammonia Lyase ◽  
Coumaric Acid ◽  
Wild Type ◽  
Km Value ◽  
Optimum Reaction ◽  
Bona Fide ◽  
Specific Activities ◽  
Bambusa Oldhamii

Phenylalanine ammonia-lyase (PAL) links the plant primary and secondary metabolisms, and its product, trans-cinnamic acid, is derived into thousands of diverse phenylpropanoids. Bambusa oldhamii BoPAL4 has broad substrate specificity using L-phenylalanine, L-tyrosine, and L-3,4-dihydroxy phenylalanine (L-DOPA) as substrates to yield trans-cinnamic acid, p-coumaric acid, and caffeic acid, respectively. The optimum reaction pH of BoPAL4 for three substrates was measured at 9.0, 8.5, and 9.0, respectively. The optimum reaction temperatures of BoPAL4 for three substrates were obtained at 50, 60, and 40 °C, respectively. The Km values of BoPAL4 for three substrates were 2084, 98, and 956 μM, respectively. The kcat values of BoPAL4 for three substrates were 1.44, 0.18, and 0.06 σ-1, respectively. The major substrate specificity site mutant, BoPAL4-H123F, showed better affinity toward L-phenylalanine by decreasing its Km value to 640 μM and increasing its kcat value to 1.87 s-1. In comparison to wild-type BoPAL4, the specific activities of BoPAL4-H123F using L-tyrosine and L-DOPA as substrates retained 5.4% and 17.8% residual activities. Therefore, L-phenylalanine, L-tyrosine, and L-DOPA are bona fide substrates for BoPAL4.

scholarly journals Propagación vegetativa  de tres especies de Bambú

Ra Ximhai ◽  
2011 ◽  
pp. 205-218
Author(s):  
Noé Lárraga-Sánchez ◽  
Nicolás Gutiérrez-Rangel ◽  
Higinio López-Sánchez ◽  
Martha E. Pedraza-Santos ◽  
Jesús Vargas-Hernández ◽  
...  
Keyword(s):  
Bambusa Vulgaris ◽  
Bambusa Oldhamii

La obtención de planta de calidad para la producción a escala comercial de bambú es un factor importante, debido a que los métodos convencionales de propagación han sido poco estudiados y limitado su propagación. El presente trabajo se desarrollo bajo condiciones de vivero, con el propósito de evaluar el efecto de los factores método de propagación (CH, V y SN), especies de bambú (Ga, Bo y Bv) y sustratos (ATC, TCE y SIC) sobre supervivencia (SPV), número de hijuelos (NH), número de raíz (NR), longitud de raíz (LR), Número de hojas por plántula (NHA), altura (AL) y diámetro del tallo (DI) del primer hijuelo de plantas de bambú, en macetas de polietileno negro. Los tratamientos se establecieron en un diseño completamente al azar con tres repeticiones. Las variables respuesta fueron evaluadas al final del experimento (136 DDT). El método por chusquin es mejor que el de vareta y segmento nodal, para las variables en estudio. La vareta y el segmento nodal ofrecen menor supervivencia y reducido número de hijuelos. Mientras que la Guadua angustifolia y Bambusa vulgaris son las mejores especies en relación a la Bambusa oldhamii para la propagación de planta de bambú, independientemente del sustrato que se utilice

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