Silver compounds regulate leaf drop and improve in vitro regeneration from mature tissues of Australian finger lime (Citrus australasica)

2020 ◽  
Vol 141 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Lamiaa M. Mahmoud ◽  
Jude W. Grosser ◽  
Manjul Dutt
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Drop ◽  
Silver Compounds

In vitro regeneration of watermelon seed segments

2015 ◽  
Vol 4 (8) ◽  
Author(s):  
M. Ameri ◽  
M. Lahouti ◽  
A. Bagheri ◽  
A. Sharifi ◽  
F. Keykha Akhar
Keyword(s):  
In Vitro Regeneration ◽  
Watermelon Seed

scholarly journals Ovaries of Chrysanthemum Irradiated with High-Energy Photons and High-Energy Electrons Can Regenerate Plants with Novel Traits

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1111
Author(s):  
Natalia Miler ◽  
Iwona Jedrzejczyk ◽  
Seweryn Jakubowski ◽  
Janusz Winiecki
Keyword(s):  
Total Dose ◽  
In Vitro Regeneration ◽  
High Energy ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
Physical Factors ◽  
Strong Impact ◽  
Breeding Method ◽  
High Energy Electrons

Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the in vitro regeneration capacity of the ovaries of two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 and 15 Gy) and high-energy electrons (total dose 10 Gy). Growth and inflorescence parameters of greenhouse acclimatized regenerants were recorded, and ploidy level was estimated with flow cytometry. The strong impact of genotype on regeneration efficiency was recorded—cultivar ‘Karolina’ produced only 7 viable shoots, while ‘Profesor Jerzy’ produced totally 428 shoots. With an increase of irradiation dose, the regeneration decreased, the least responsive were explants irradiated with 15 Gy high-energy photons and 10 Gy high-energy electrons. Regenerants of ‘Profesor Jerzy’ obtained from these explants possessed shorter stem and flowered later. The highest number of stable, color and shape inflorescence variations were obtained from explants treated with 10 Gy high-energy photons. Variations of inflorescences were predominantly changes of shape—from full to semi-full. New color phenotypes were dark yellow, light yellow and pinkish, among them only the dark yellow phenotype remained stable during second year cultivation. None of the regenerants were haploid. The application of ovaries irradiated within the whole inflorescence of chrysanthemum can be successfully applied in the breeding programs, provided the mother cultivar regenerate in vitro efficiently.

In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system

2021 ◽  
Vol 57 (2) ◽  
pp. 225-234
Author(s):  
Rongpei Yu ◽  
Fan Li ◽  
Guoxian Wang ◽  
Jiwei Ruan ◽  
Lifang Wu ◽  
...  
Keyword(s):  
In Vitro Regeneration

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

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