scholarly journals Long-term subculture affects rooting competence via changes in the hormones and protein profiles in Cedrela fissilis Vell. (Meliaceae) shoots

2021 ◽  
Author(s):  
Tadeu dos Reis de Oliveira ◽  
Damián Balfagón ◽  
Kariane Rodrigues de Sousa ◽  
Victor Paulo Mesquita Aragão ◽  
Leandro Francisco de Oliveira ◽  
...  
Keyword(s):  
Protein Profiles ◽  
Rooting Competence

Effect of long‐term storage on phenolic composition, antioxidant capacity, and protein profiles of Calicotome villosa subsp. intermedia seeds

2019 ◽  
Vol 44 (1) ◽  
Author(s):  
Fayçal Boughalleb ◽  
Maher Mahmoudi ◽  
Raoudha Abdellaoui ◽  
Boutheina Yahia ◽  
Slah Zaidi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Protein Profiles ◽  
Phenolic Composition ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Long-Term Subculture Affects Rooting Competence via Changes in the Hormones and Protein Profiles in Cedrela Fissilis Vell. (Meliaceae) Shoots

2021 ◽  
Author(s):  
Tadeu dos Reis Oliveira ◽  
Damián Balfagón ◽  
Kariane Rodrigues Sousa ◽  
Victor Paulo Mesquita Aragão ◽  
Leandro Francisco de Oliveira ◽  
...  
Keyword(s):  
Large Scale ◽  
Molecular Mechanisms ◽  
Monodehydroascorbate Reductase ◽  
Protein Profiles ◽  
Ex Vitro ◽  
Plantlet Production ◽  
Auxin Binding Protein ◽  
Indole 3 Acetic Acid

Abstract Long-term subculture plays an essential role in the large-scale multiplication and production of somatic plantlets. We investigated the effects of long-term subculture on in vitro shoot development and ex vitro rooting associated with changes in the hormones and protein profiles in C. fissilis. The number of subcultures of shoots induced a decrease in the ex vitro rooting response. The reduction in adventitious root (AR) formation was associated with decreases in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), 12-oxo phytodienoic acid (OPDA), putrescine (Put), and spermine and increases in jasmonic acid (JA), jasmonoyl-isoleucine, trans-cinnamic acid, and salicylic acid contents in shoots at the fourth subculture compared to the first. The ornithine decarboxylase enzyme preferentially functions in the Put biosynthesis pathway and was related to the highest AR formation in shoots at the first subculture. Down-accumulation of the auxin-binding protein ABP19a in shoots from the fourth subculture compared to the first subculture was related to a decrease in both IAA contents and AR formation. In addition, down-accumulation of glucose-6-phosphate isomerase, glutamine synthetase leaf isozyme chloroplastic, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, L-ascorbate peroxidase, cytosolic, monodehydroascorbate reductase, and 2-Cys peroxiredoxin BAS1-like, chloroplastic and up-accumulation of caffeoyl-CoA O-methyltransferase 1 and isoforms of peroxidase 4 proteins in shoots from the fourth relative to the first subculture were associated with a reduction in AR formation. These results showed that the understanding of hormonal and molecular mechanisms related to the potential of AR formation in shoots under successive subcultures is relevant to improving large-scale plantlet production in C. fissilis.

Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling

2005 ◽  
Vol 43 (2) ◽  
Author(s):  
Manuel Aivado ◽  
Dimitrios Spentzos ◽  
Gil Alterovitz ◽  
Hasan H. Otu ◽  
Franck Grall ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Protein Profiles ◽  
Protein Arrays ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced

AbstractSurface-enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry with protein arrays has facilitated the discovery of disease-specific protein profiles in serum. Such results raise hopes that protein profiles may become a powerful diagnostic tool. To this end, reliable and reproducible protein profiles need to be generated from many samples, accurate mass peak heights are necessary, and the experimental variation of the profiles must be known. We adapted the entire processing of protein arrays to a robotics system, thus improving the intra-assay coefficients of variation (CVs) from 45.1% to 27.8% (p<0.001). In addition, we assessed up to 16 technical replicates, and demonstrated that analysis of 2–4 replicates significantly increases the reliability of the protein profiles. A recent report on limited long-term reproducibility seemed to concord with our initial inter-assay CVs, which varied widely and reached up to 56.7%. However, we discovered that the inter-assay CV is strongly dependent on the drying time before application of the matrix molecule. Therefore, we devised a standardized drying process and demonstrated that our optimized SELDI procedure generates reliable and long-term reproducible protein profiles with CVs ranging from 25.7% to 32.6%, depending on the signal-to-noise ratio threshold used.

scholarly journals Parallel Changes in Global Protein Profiles During Long-Term Experimental Evolution inEscherichia coli

Genetics ◽  
2006 ◽  
Vol 173 (4) ◽  
pp. 1851-1869 ◽  
Author(s):  
Ludovic Pelosi ◽  
Lauriane Kühn ◽  
Dorian Guetta ◽  
Jérôme Garin ◽  
Johannes Geiselmann ◽  
...  
Keyword(s):  
Experimental Evolution ◽  
Inescherichia Coli ◽  
Protein Profiles

An Evaluation of Serum Protein Profiles in the Long-Term Surveillance of Prostatic Cancer

1980 ◽  
Vol 14 (2) ◽  
pp. 143-149 ◽  
Author(s):  
K. Trautner ◽  
E. H. Cooper ◽  
Sarah Haworth ◽  
A. Milford Ward
Keyword(s):  
Serum Protein ◽  
Prostatic Cancer ◽  
Protein Profiles

Therapy and prevention for mental health: What if mental diseases are mostly not brain disorders?

2019 ◽  
Vol 42 ◽  
Author(s):  
John P. A. Ioannidis
Keyword(s):  
Mental Health ◽  
Mental Disease ◽  
Brain Disorders ◽  
Social Stressors ◽  
Labor Education ◽  
Mental Diseases ◽  
Long Term Follow Up ◽  
Political Decisions

AbstractNeurobiology-based interventions for mental diseases and searches for useful biomarkers of treatment response have largely failed. Clinical trials should assess interventions related to environmental and social stressors, with long-term follow-up; social rather than biological endpoints; personalized outcomes; and suitable cluster, adaptive, and n-of-1 designs. Labor, education, financial, and other social/political decisions should be evaluated for their impacts on mental disease.

Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

2016 ◽  
Vol 39 ◽  
Author(s):  
Mary C. Potter
Keyword(s):  
Working Memory ◽  
Rapid Serial Visual Presentation ◽  
Language Comprehension ◽  
Short Term Memory ◽  
Visual Presentation ◽  
Long Term Memory ◽  
Short Term ◽  
Term Memory ◽  
Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

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