Unexpected effects of chitinases on the peach-potato aphid (Myzus persicae Sulzer) when delivered via transgenic potato plants (Solanum tuberosum Linn�) and in vitro

2005 ◽  
Vol 14 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Julien Saguez ◽  
Romaric Hainez ◽  
Anas Cherqui ◽  
Olivier Van Wuytswinkel ◽  
Haude Jeanpierre ◽  
...  
1996 ◽  
Vol 79 (3) ◽  
pp. 295-307 ◽  
Author(s):  
Angharad M. R. Gatehouse ◽  
Rachel E. Down ◽  
Kevin S. Powell ◽  
Nicolas Sauvion ◽  
Yvan Rahbé ◽  
...  

2015 ◽  
Vol 125 ◽  
pp. 214-223 ◽  
Author(s):  
Luybov A. Wasserman ◽  
Andrey I. Sergeev ◽  
Viktor G. Vasil’ev ◽  
Irina G. Plashchina ◽  
Nina P. Aksenova ◽  
...  

2018 ◽  
Vol 22 ◽  
pp. 299-304
Author(s):  
O. O. Ovcharenko ◽  
V. A. Rudas ◽  
N. L. Shcherbak ◽  
M. V. Kuchuk

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.


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