Seroprevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic zones of Himachal Pradesh, India

Abstract Trypanosoma evansi, a hemoflagellate protozoan parasite causes wasting disease called surra in wide range of animals. Although, the organism has been reported from various parts of the India, data generated from organized epidemiological study is still in infancy in majority states of India. In the present study, livestock of Himachal Pradesh, India was targeted for epidemiological investigation of T. evansi infections. A total of 440 equines and 444 cattle serum samples were collected from four agro-climatic Zones. Further, serum samples of 280 buffaloes from three different agro-climatic Zones of Himachal Pradesh were also collected and evaluated for presence of T. evansi infection by indirect ELISA. Data generated showed higher prevalence in buffalo (23.57%) followed by cattle (22.52%) and equines (1.82%). Disease was found to be more prevalent (P < 0.05) in cattle of lower altitude as compared to those of higher altitudes. No significant variation was seen in prevalence of disease on the basis of age and sex of the animals. Serum biochemical analysis revealed increased levels of BUN in T. evansi infected equines. Levels of liver function enzymes such as ALT/GGT and AST were found to be significantly elevated (P < 0.01) in infected animals whereas glucose levels were significantly lower in surra infected animals as compared to non-infected animals. Western blot analysis of whole cell lysate (WCL) antigen of T. evansi using surra infected serum samples of equines showed immunodominant bands in the range of 100-25 kDa. Surra infected bovine serum samples recognized polypeptide bands in the range of 85-32 kDa, including protein clusters of 52-55 and 48-46 kDa. Poypeptide cluster of 62-66 kDa was found common to serum samples of bovines and equines from all agro-climatic Zones. Animal trypanosomosis was found to be highly prevalent in livestock of Himachal Pradesh and thus there is dire need for designing of proper control strategies against surra.

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Biochemical and immunological characterization of alpha-amylase isoenzymes of Araucaria araucana

Physiologia Plantarum ◽

10.1034/j.1399-3054.1994.920120.x ◽

1994 ◽

Vol 92 (1) ◽

pp. 149-159

Author(s):

Elba Acevedo ◽

Liliana Cardemil

Keyword(s):

Alpha Amylase ◽

Immunological Characterization ◽

Araucaria Araucana ◽

Amylase Isoenzymes

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The Immunological Characterization of Human Antibodies to Factor VIII Isolated by Immuno-Affinity Chromatography

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650129 ◽

1981 ◽

Vol 45 (01) ◽

pp. 060-064 ◽

Cited By ~ 10

Author(s):

M L Kavanagh ◽

C N Wood ◽

J F Davidson

Keyword(s):

Factor Viii ◽

Affinity Chromatography ◽

Gel Filtration ◽

Immunological Characterization ◽

Human Antibodies ◽

Heavy Chains ◽

Light Chain Type ◽

Antibody Preparations ◽

Chain Type

SummaryNine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.

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