Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic

Virus Genes ◽  
2009 ◽  
Vol 38 (3) ◽  
pp. 468-474 ◽  
Author(s):  
J. K. Kundu ◽  
S. Gadiou ◽  
G. Červená
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
The Czech Republic

Wheat dwarf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Triticum Aestivum ◽  
Czech Republic ◽  
Hordeum Vulgare ◽  
Avena Sativa ◽  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
Distribution Map ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Wheat dwarf virus. Geminiviridae: Mastrevirus. Hosts: wheat (Triticum aestivum), barley (Hordeum vulgare), oat (Avena sativa). Information is given on the geographical distribution in Europe (Austria, Bulgaria, Czech Republic, Finland, France, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, Spain, Sweden, UK, Ukraine), Asia (China, Hebei, Qinghai, Shaanxi, Shanxi, Sichuan, Yunnan, Iran, Syria, Turkey), Africa (Tunisia Zambia).

scholarly journals Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Cold Resistance ◽  
The Czech Republic ◽  
Baking Quality ◽  
The Individual

 Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.

RASPBERRY BUSHY DWARF VIRUS IN THE CZECH REPUBLIC

1998 ◽  
pp. 75-78
Author(s):  
J. Å pak ◽  
D. Kubelková ◽  
M. Janeçková
Keyword(s):  
Czech Republic ◽  
Dwarf Virus ◽  
The Czech Republic ◽  
Raspberry Bushy Dwarf Virus

Microsatellite-based genetic diversity and differentiation of foreign common carp (Cyprinus carpio) strains farmed in the Czech Republic

Aquaculture ◽  
2010 ◽  
Vol 298 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Martin Hulak ◽  
Vojtech Kaspar ◽  
Klaus Kohlmann ◽  
Kevin Coward ◽  
Jakub Tešitel ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
The Czech Republic ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals Characterization of Czech hop (Humulus lupulus L.) genotypes by molecular methods

2011 ◽  
Vol 48 (No. 8) ◽  
pp. 343-350 ◽  
Author(s):  
J. Patzak
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Humulus Lupulus ◽  
Quality System ◽  
Molecular Methods ◽  
Germplasm Management ◽  
Genetic Studies ◽  
The Czech Republic ◽  
Humulus Lupulus L

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

2015 ◽  
Vol 114 (4) ◽  
pp. 1619-1624 ◽  
Author(s):  
Pavla Wagnerová ◽  
Bohumil Sak ◽  
John McEvoy ◽  
Michael Rost ◽  
Agniezska Perec Matysiak ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
The Czech Republic ◽  
Cryptosporidium Spp ◽  
Cryptosporidium Muris

scholarly journals First Report of Barley yellow dwarf virus-MAV in Oat, Wheat, and Barley Grown in the Czech Republic

Plant Disease ◽  
2009 ◽  
Vol 93 (9) ◽  
pp. 964-964 ◽  
Author(s):  
J. K. Kundu
Keyword(s):  
Czech Republic ◽  
Barley Yellow Dwarf Virus ◽  
Virus Disease ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Cereal Crops ◽  
Rt Pcr ◽  
The Czech Republic ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Barley yellow dwarf disease, a ubiquitous virus disease of cereal crops worldwide, is caused by a group of related, single-stranded RNA viruses assigned to Luteovirus (Barley yellow dwarf virus [BYDV] spp. PAV, PAS, MAV, and GAV) or Polerovirus (Cereal yellow dwarf virus-RPV) genera or unassigned to a genera (BYDV-SGV, BYDV-RMV, and BYDV-GPV) in the family Luteoviridae (1). Incidence of BYDV in cereal crops (e.g., barley, wheat, and oats) was high, and in recent years, reached epidemic levels in many regions of the Czech Republic. BYDV-PAV and BYDV-PAS have been identified in Czech cereal crops (2,4). Surveys of the incidence of BYDV were carried out using ELISA (SEDIAG SAS, Longvic, France) and one-step reverse transcription (RT)-PCR (Qiagen, Hilden, Germany) (2) during 2007 and 2008. Samples (125) were collected from different fields around the Czech Republic and 96 were BYDV positive. Three of the field isolates, CZ-6815, CZ-1561, and CZ-10844, from oat (Avena sativa; cv. Auron), winter wheat (Triticum aestivum; cv. Apache), and winter barley (Hordeum vulgare; cv. Merlot), respectively, were identified as BYDV-MAV by sequencing of the RT-PCR product (641-bp fragment) used to identify BYDV, which spanned 2839–3479 of the BYDV genome (GenBank Accession Nos. EF043235 and NC_002160) (2). The partial coat protein gene sequence of 483 nt was compared with the available sequences of 12 BYDV-PAV isolates (PAV-JP, PAV-NY, PAV-ILL, PAV-AUS, PAV-WG2, PAV-whG4y3, PAV-on21-4, Tahoe1, CA-PAV, HB3, FH3, and MA9501); nine BYDV-PAS isolates (PAS-129, PAS-64, WS6603, WG13, PAS-Tcb4-1, PASwaw5-9, FL2, PAS-Vd29, and PAS-MA9516); and six BYDV-MAV isolates (MAV-CA, MAV-PS1X1, MAV-Alameds268, LMB2a, SI-o4, and MAV-CN) by MEGA4 (3). Nucleotide and amino acid sequence identities for the three isolates ranged from 92.9 to 99.4% and 88.0 to 95.8%, respectively, for available BYDV-MAV isolates; 76.8 to 78.2% and 62.7 to 67.6%, respectively, for available BYDV-PAS isolates; and 77.6 to 79.3% and 65.5 to 70.4%, respectively, for available PAV isolates. The sequence data indicates that these isolates (CZ-6815, CZ-1561, and CZ10844; GenBank Accession Nos. FJ645747, FJ645758, and FJ645746, respectively) are BYDV-MAV. To my knowledge, this is the first record of BYDV-MAV in the Czech Republic. References: (1) C. J. D'Arcy and L. L. Domier. Page 891 in: Virus Taxonomy-8th Report of the ICTV. C. M. Fauquet et al., eds. Springer-Verlag, NY, 2005. (2) J. K. Kundu. Plant Dis. 92:1587, 2008. (3) K. Tamura et al. Mol. Biol. Evol. 24:1596, 2007. (4) J. Vacke. Page 100 in: Sbornik Referatu z Odborneho Seminare, Aktualni Problemy Ochrany Polnich Plodin, Praha, 1991.

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