scholarly journals Use of nuclear microsatellite loci for evaluating genetic diversity among selected populations of Abies alba Mill. in the Czech Republic

2016 ◽  
Vol 61 (No. 8) ◽  
pp. 345-351 ◽  
Author(s):  
H. Cvrčková ◽  
P. Máchová ◽  
J. Malá
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Microsatellite Loci ◽  
Abies Alba ◽  
The Czech Republic ◽  
Nuclear Microsatellite

scholarly journals Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Cold Resistance ◽  
The Czech Republic ◽  
Baking Quality ◽  
The Individual

 Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.

scholarly journals Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci

2019 ◽  
Vol 30 (1) ◽  
pp. 54
Author(s):  
T Hussain ◽  
M M. Musthafa ◽  
M E. Babar ◽  
M Shaheen ◽  
F M. Marikar
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Molecular Genetic ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Molecular Genetic Diversity ◽  
Nuclear Microsatellite ◽  
Relationship Of

<p>En Pakistán los recursos genéticos ovinos son abundantes, disponiéndose de varias diferentes castas extendidas en todo el país. No obstante, la diversidad molecular de las razas de ovejas es poco conocida en la región. Por ello, en el presente estudio se investigaron 16 marcadores microsatelitales de las razas indígenas Buchi y Hashtnagri, en las provincias de Pendjab y Balochistan respectivamente. Para indagar la diversidad genética se obtuvieron muestras de sangre de 25 animales de cada raza. El promedio del número de alelos en Buchi y Hashtnagri fue de 3,375±1,455 y 3,50±1,591 respectivamente. Los promedios de heterocigosis registradas fueron de 0,878±0,204 para la raza Buchi y 0,885±0,218 para Hashtnagri. Los índices de Shannon fueron 1,032±0,371 para Buchi y 1,070±0,412 para Hashtnagri, respectivamente. La estimación de los coeficientes de endogamia (FIS y FIT) mostró valores negativos, en tanto que el flujo genético (migración) fue de 10,09 y el promedio de la diferencia poblacional (FST) resultó de 2,4%. En ambas razas, el índice de información polimórfica fue 0,56, indicando el valor del marcador del tablero. Entre las ovejas Buchi y Hashtnagri, la distancia genética estándar de Nei (Ds) fue 0,0218. Según estos resultados, ambas razas mostraron considerable diversidad genética. Los datos obtenidos permiten vislumbrar una promisoria mejoría en el área de la conservación y en el diseño de las estrategias para la cría de ovejas en el futuro cercano.</p>

Microsatellite-based genetic diversity and differentiation of foreign common carp (Cyprinus carpio) strains farmed in the Czech Republic

Aquaculture ◽  
2010 ◽  
Vol 298 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Martin Hulak ◽  
Vojtech Kaspar ◽  
Klaus Kohlmann ◽  
Kevin Coward ◽  
Jakub Tešitel ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
The Czech Republic ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals Characterization of Czech hop (Humulus lupulus L.) genotypes by molecular methods

2011 ◽  
Vol 48 (No. 8) ◽  
pp. 343-350 ◽  
Author(s):  
J. Patzak
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Humulus Lupulus ◽  
Quality System ◽  
Molecular Methods ◽  
Germplasm Management ◽  
Genetic Studies ◽  
The Czech Republic ◽  
Humulus Lupulus L

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

2015 ◽  
Vol 114 (4) ◽  
pp. 1619-1624 ◽  
Author(s):  
Pavla Wagnerová ◽  
Bohumil Sak ◽  
John McEvoy ◽  
Michael Rost ◽  
Agniezska Perec Matysiak ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
The Czech Republic ◽  
Cryptosporidium Spp ◽  
Cryptosporidium Muris

Formalized classification of the vegetation of Abies alba-dominated forests in the Czech Republic

Biologia ◽  
2010 ◽  
Vol 65 (5) ◽  
Author(s):  
Karel Boublík
Keyword(s):  
Czech Republic ◽  
Abies Alba ◽  
Single Species ◽  
Soil Reaction ◽  
Silver Fir ◽  
Herb Layer ◽  
The Czech Republic ◽  
Logical Operators ◽  
Tree Layer

AbstractA phytosociological classification of silver fir (Abies alba) forests in the Czech Republic was carried out using the Braun-Blanquet approach. It is based on the formalized and supervised Cocktail classification method. The definitions of associations were created by combinations of sociological species groups using logical operators. Dominance of single species was included in the definitions of associations. All relevés with at least 50% cover of silver fir in tree layer available from the Czech Republic were used for analysis. Three associations within two classes were distinguished. Vaccinio vitis-idaeae-Abietetum albae (Vaccinio-Piceetea, Piceion excelsae) is an oligotrophic, species-poor spruce-pine-fir forest dominated by Vaccinium myrtillus in the herb layer occurring mostly on podzols. Luzulo-Abietetum albae (Querco-Fagetea, Luzulo-Fagion) is an oligo-mesotrophic spruce-fir forest characterized by the dominance of graminoids (Luzula luzuloides, Calamagrostis arundinacea) in the herb layer. It occurs especially on cambisols. Nutrient-demanding species are typical of the stands of herb-rich mesotrophic Galio rotundifolii-Abietetum albae (Querco-Fagetea, Fagion sylvaticae, Galio rotundifolii-Abietenion) that occurs also mostly on cambisols. Soil nutrients and soil reaction (represented by Ellenberg indicator values) were determined as the most important ecological gradients affecting the variation of the vegetation in these communities.

Identification and characterization of nuclear microsatellite loci in Abies alba Mill.

2006 ◽  
Vol 6 (2) ◽  
pp. 374-376 ◽  
Author(s):  
E. CREMER ◽  
S. LIEPELT ◽  
F. SEBASTIANI ◽  
A. BUONAMICI ◽  
I. M. MICHALCZYK ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Abies Alba ◽  
Nuclear Microsatellite ◽  
Identification And Characterization
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