scholarly journals Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma

2013 ◽  
Vol 56 (8) ◽  
pp. 720-730 ◽  
Author(s):  
Qi Zhang ◽  
Helen Vuong ◽  
Xin Huang ◽  
YanLing Wang ◽  
Nicholas C. Brecha ◽  
...  
Keyword(s):  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Loss ◽  
Behavioral Analysis ◽  
Retinal Ganglion

scholarly journals Quantification and image-derived phenotyping of retinal ganglion cell nuclei in the nee mouse model of congenital glaucoma

2021 ◽  
Author(s):  
Carly J. van der Heide ◽  
Kacie J. Meyer ◽  
Hannah E. Mercer ◽  
Adam Hedberg-Buenz ◽  
Michael G. Anderson
Keyword(s):  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Time Course ◽  
Cell Loss ◽  
Average Density ◽  
Congenital Glaucoma ◽  
Retinal Ganglion ◽  
Cell Nuclei ◽  
Significant Difference

ABSTRACTThe nee mouse model exhibits characteristic features of congenital glaucoma, a common cause of childhood blindness. The current study of nee mice had two components. First, the time course of neurodegeneration in nee retinal flat-mounts was studied over time using a retinal ganglion cell (RGC)-marker, BRN3A; a pan-nuclear marker, TO-PRO-3; and H&E staining. Based on segmentation of nuclei using ImageJ and RetFM-J, this analysis identified a rapid loss of BRN3A+ nuclei from 4–15 weeks of age, with the first statistically significant difference in average density compared to age-matched controls detected in 8-week-old cohorts (49% reduction in nee). Consistent with a model of glaucoma, no reductions in BRN3A− nuclei were detected, but the combined analysis indicated that some RGCs lost BRN3A marker expression prior to actual cell loss. These results have a practical application in the design of experiments using nee mice to study mechanisms or potential therapies for congenital glaucoma. The second component of the study pertains to a discovery-based analysis of the large amount of image data with 748,782 segmented retinal nuclei. Using the automatedly collected region of interest feature data captured by ImageJ, we tested whether RGC density of glaucomatous mice was significantly correlated to average nuclear area, perimeter, Feret diameter, or MinFeret diameter. These results pointed to two events influencing nuclear size. For variations in RGC density above approximately 3,000 nuclei/mm2 apparent spreading was observed, in which BRN3A− nuclei—regardless of genotype—became slightly larger as RGC density decreased. This same spreading occurred in BRN3A+ nuclei of wild-type mice. For variation in RGC density below 3,000 nuclei/mm2, which only occurred in glaucomatous nee mutants, BRN3A+ nuclei became smaller as disease was progressively severe. These observations have relevance to defining RGCs of relatively higher sensitivity to glaucomatous cell death and the nuclear dynamics occurring during their demise.

scholarly journals Tumor Necrosis Factor-  Mediates Oligodendrocyte Death and Delayed Retinal Ganglion Cell Loss in a Mouse Model of Glaucoma

2006 ◽  
Vol 26 (49) ◽  
pp. 12633-12641 ◽  
Author(s):  
T. Nakazawa ◽  
C. Nakazawa ◽  
A. Matsubara ◽  
K. Noda ◽  
T. Hisatomi ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Tumor Necrosis ◽  
Cell Loss ◽  
Retinal Ganglion ◽  
Necrosis Factor

scholarly journals OPA1 gene therapy prevents retinal ganglion cell loss in a Dominant Optic Atrophy mouse model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Emmanuelle Sarzi ◽  
Marie Seveno ◽  
Camille Piro-Mégy ◽  
Lucie Elzière ◽  
Mélanie Quilès ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Optic Atrophy ◽  
Cell Loss ◽  
Retinal Ganglion ◽  
Dominant Optic Atrophy ◽  
Opa1 Gene

XIAP gene therapy effects on retinal ganglion cell structure and function in a mouse model of glaucoma

Gene Therapy ◽  
2021 ◽  
Author(s):  
Shagana Visuvanathan ◽  
Adam N. Baker ◽  
Pamela S. Lagali ◽  
Stuart G. Coupland ◽  
Garfield Miller ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Structure ◽  
Structure And Function ◽  
Retinal Ganglion ◽  
And Function

scholarly journals Estimation of Retinal Ganglion Cell Loss in Glaucomatous Eyes With a Relative Afferent Pupillary Defect

2014 ◽  
Vol 55 (1) ◽  
pp. 513 ◽  
Author(s):  
Andrew J. Tatham ◽  
Daniel Meira-Freitas ◽  
Robert N. Weinreb ◽  
Amir H. Marvasti ◽  
Linda M. Zangwill ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Loss ◽  
Retinal Ganglion ◽  
Relative Afferent Pupillary Defect

scholarly journals Retinal ganglion cell loss in kinesin-1 cargo Alcadein α deficient mice

2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Yuki Nakano ◽  
Kazuyuki Hirooka ◽  
Yoichi Chiba ◽  
Masaki Ueno ◽  
Daiki Ojima ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Loss ◽  
Retinal Ganglion ◽  
Deficient Mice

scholarly journals Melanopsin retinal ganglion cell loss in A lzheimer disease

2015 ◽  
Vol 79 (1) ◽  
pp. 90-109 ◽  
Author(s):  
Chiara La Morgia ◽  
Fred N. Ross‐Cisneros ◽  
Yosef Koronyo ◽  
Jens Hannibal ◽  
Roberto Gallassi ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Loss ◽  
Retinal Ganglion

Rapamycin mediates mTOR signaling in reactive astrocytes and reduces retinal ganglion cell loss

2018 ◽  
Vol 176 ◽  
pp. 10-19 ◽  
Author(s):  
Ningfeng Li ◽  
Feifei Wang ◽  
Qinglin Zhang ◽  
Ming Jin ◽  
Ye Lu ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Loss ◽  
Reactive Astrocytes ◽  
Mtor Signaling ◽  
Retinal Ganglion
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