scholarly journals Protective immunity against Rickettsia heilongjiangensis in a C3H/HeN mouse model mediated by outer membrane protein B-pulsed dendritic cells

2014 ◽  
Vol 58 (3) ◽  
pp. 287-296 ◽  
Author(s):  
YanFen Meng ◽  
XiaoLu Xiong ◽  
Yong Qi ◽  
ChangSong Duan ◽  
WenPing Gong ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Membrane Protein ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protective Immunity ◽  
Rickettsia Heilongjiangensis ◽  
Protein B

scholarly journals Outer membrane protein A renders dendritic cells and macrophages responsive to CCL21 and triggers dendritic cell migration to secondary lymphoid organs

2003 ◽  
Vol 33 (2) ◽  
pp. 326-333 ◽  
Author(s):  
Pascale Jeannin ◽  
Giovanni Magistrelli ◽  
Nathalie Herbault ◽  
Liliane Goetsch ◽  
Sylvie Godefroy ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cell Migration ◽  
Dendritic Cell ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein A ◽  
Dendritic Cell Migration ◽  
Outer Membrane Protein A ◽  
Secondary Lymphoid Organs

scholarly journals Yersinia outer-membrane protein B (YopB): a tool for identification of Yersinia pestis isolates

2006 ◽  
Vol 55 (4) ◽  
pp. 467-469 ◽  
Author(s):  
Rekha Khushiramani ◽  
Jyoti Shukla ◽  
Urmil Tuteja ◽  
Harsh Vardhan Batra
Keyword(s):  
Membrane Protein ◽  
Yersinia Pestis ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein B

scholarly journals Enhancement of Pulmonary Clearance ofMoraxella (Branhamella) catarrhalisfollowing Immunization with Outer Membrane Protein CD in a Mouse Model

1998 ◽  
Vol 178 (6) ◽  
pp. 1667-1675 ◽  
Author(s):  
Timothy F. Murphy ◽  
Jennelle M. Kyd ◽  
Anna John ◽  
Charmaine Kirkham ◽  
Allan W. Cripps
Keyword(s):  
Membrane Protein ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Pulmonary Clearance

scholarly journals Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22663 ◽  
Author(s):  
Debasis Pore ◽  
Nibedita Mahata ◽  
Amit Pal ◽  
Manoj K. Chakrabarti
Keyword(s):  
Immune Response ◽  
Membrane Protein ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Shigella Flexneri ◽  
Protein A ◽  
Protective Immune Response ◽  
Shigella Flexneri 2A ◽  
Outer Membrane Protein A

scholarly journals Serological Reactivity and Biochemical Characterization of Methylated and Unmethylated Forms of a Recombinant Protein Fragment Derived from Outer Membrane Protein B of Rickettsia typhi

2008 ◽  
Vol 15 (4) ◽  
pp. 684-690 ◽  
Author(s):  
Chien-Chung Chao ◽  
Zhiwen Zhang ◽  
Hui Wang ◽  
Abdulnaser Alkhalil ◽  
Wei-Mei Ching
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Mass Spectrometry Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Composition Analysis ◽  
Rickettsia Typhi ◽  
Serological Reactivity ◽  
Lysine Residues ◽  
Protein B

ABSTRACT Rickettsia typhi, an obligate intracellular bacterium that causes murine typhus, possesses a heavily methylated outer membrane protein B (OmpB) antigen. This immunodominant antigen is responsible for serological reactions and is capable of eliciting protective immune responses with a guinea pig model. Western blot analysis of partially digested OmpB with patient sera revealed that most of the reactive fragments are larger than 20 kDa. One of these fragments, which is located at the N terminus (amino acids 33 to 273), fragment A (At), has been expressed in Escherichia coli. The expressed protein (rAt) was purified by chromatography and properly refolded by sequential dialysis. The refolded rAt protein was recognized by at least 87% of the typhus group patient sera as determined by enzyme-linked immunosorbent assay (ELISA). However, the titers were lower than those obtained with OmpB of R. typhi. Since native OmpB is hypermethylated at lysine residues, we chemically methylated the lysine residues in rAt. The methylation was confirmed by amino acid composition analysis, and the methylation pattern of the methylated rAt (mrAt) protein was similar to that of native At from OmpB, as revealed by liquid chromatography-mass spectrometry analysis. Both rAt and mrAt were evaluated in an ELISA for their serological reactivity with patient sera. Among patient sera tested, 83% exhibited higher titers with mrAt than with rAt. These results suggest that rAt, with or without methylation, can potentially replace rickettsia-derived OmpB or whole-cell antigen for the diagnosis of R. typhi infection.

scholarly journals Polymorphism of Repeated Regions of Pertactin in Bordetella pertussis, Bordetella parapertussis, andBordetella bronchiseptica

2000 ◽  
Vol 68 (8) ◽  
pp. 4815-4817 ◽  
Author(s):  
Caroline Boursaux-Eude ◽  
Nicole Guiso
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Outer Membrane Protein ◽  
Protective Immunity ◽  
Bordetella Bronchiseptica ◽  
Bordetella Parapertussis ◽  
Region I ◽  
Region Ii

ABSTRACT Pertactin is an outer membrane protein expressed byBordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica that induces protective immunity to Bordetella infections. The immunodominant and immunoprotective epitopes of pertactin include two repeated regions, I and II. Comparison of these two repeated regions showed that B. parapertussis pertactin is invariant, whereas B. pertussis pertactin varies mostly in region I and B. bronchiseptica pertactin varies in both repeated regions I and II, but mostly in region II. These differences may result from specific characteristics of these Bordetella species.

Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

Vaccine ◽  
2001 ◽  
Vol 20 (5-6) ◽  
pp. 979-988 ◽  
Author(s):  
P.A Crocquet-Valdes ◽  
C.M Dı́az-Montero ◽  
H.M Feng ◽  
H Li ◽  
A.D.T Barrett ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protective Immunity ◽  
Spotted Fever ◽  
Protein A ◽  
Outer Membrane Protein A
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