Critical Role of Beclin1 in HIV Tat and Morphine-Induced Inflammation and Calcium Release in Glial Cells from Autophagy Deficient Mouse

Calcium release from cultured bone is pH dependent; net calcium flux (JCa) from bone increases with decreasing pH. At a similar decrement in pH there is greater JCa when acidosis is produced by a low medium bicarbonate concentration ([HCO3-]), a model of metabolic acidosis (Met), compared with an increased medium PCO2, a model of respiratory acidosis (Resp). To separate the role of [HCO3-] from that of pH in inducing JCa we cultured calvariae for 3 h under three different neutral (pH approximately 7.4) isohydric environments [control (Ctl), fully compensated Met (C-Met), or fully compensated Resp (C-Resp)] and two different acid (pH approximately 7.1) isohydric environments (Met or Resp). During neutral pH (Ctl, C-Met, and C-Resp) there was JCa from bone during C-Met (decreased [HCO3-]), no net flux during Ctl (normal [HCO3-]), and JCa into bone during C-Resp (increased [HCO3-]); and JCa was correlated inversely with [HCO3-] (r = -0.824, n = 36, P less than 0.001). During acid pH there was greater JCa from bone during Met (decreased [HCO3-]) than during Resp (normal [HCO3-]); and JCa was again correlated inversely with [HCO3-] (r = -0.848, n = 22, P less than 0.001). JCa from bone during Met and Resp was greater than C-Met and C-Resp, respectively. The addition of the osteoclastic inhibitor salmon calcitonin did not alter the relative JCa results. Thus at a constant pH the magnitude of JCa from cultured neonatal mouse calvariae appears dependent on the [HCO3-]; the lower the [HCO3-], the greater the calcium efflux.(ABSTRACT TRUNCATED AT 250 WORDS)

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Glutamate-triggered calcium signalling in mouse Bergmann glial cells in situ: role of inositol-1,4,5-trisphosphate-mediated intracellular calcium release

Neuroscience ◽

10.1016/s0306-4522(99)00067-6 ◽

1999 ◽

Vol 92 (3) ◽

pp. 1051-1059 ◽

Cited By ~ 54

Author(s):

S. Kirischuk ◽

F. Kirchhoff ◽

V. Matyash ◽

H. Kettenmann ◽

A. Verkhratsky

Keyword(s):

Intracellular Calcium ◽

Glial Cells ◽

Calcium Release ◽

Calcium Signalling ◽

Inositol 1,4,5 Trisphosphate ◽

Bergmann Glial Cells ◽

Intracellular Calcium Release

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The Role of Satellite Glial Cells, Astrocytes, and Microglia in Oxaliplatin-Induced Neuropathic Pain

Biomedicines ◽

10.3390/biomedicines8090324 ◽

2020 ◽

Vol 8 (9) ◽

pp. 324 ◽

Cited By ~ 1

Author(s):

Ji Hwan Lee ◽

Woojin Kim

Keyword(s):

Neuropathic Pain ◽

Glial Cells ◽

Therapeutic Option ◽

Critical Role ◽

Satellite Glial Cells ◽

In Vivo Experiments ◽

Effective Therapeutic Option

Oxaliplatin is a third-generation platinum-based chemotherapeutic drug. Although its efficacy against colorectal cancer is well known, peripheral neuropathy that develops during and after infusion of the agents could decrease the quality of life of the patients. Various pathways have been reported to be the cause of the oxaliplatin-induced paresthesia and dysesthesia; however, its mechanism of action has not been fully understood yet. In recent years, researchers have investigated the function of glia in pain, and demonstrated that glia in the peripheral and central nervous system could play a critical role in the development and maintenance of neuropathic pain. These results suggest that targeting the glia may be an effective therapeutic option. In the past ten years, 20 more papers focused on the role of glia in oxaliplatin-induced thermal and mechanical hypersensitivity. However, to date no review has been written to summarize and discuss their results. Thus, in this study, by reviewing 23 studies that conducted in vivo experiments in rodents, the change of satellite glial cells, astrocytes, and microglia activation in the dorsal root ganglia, spinal cord, and the brain of oxaliplatin-induced neuropathic pain animals is discussed.

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The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision

eLife ◽

10.7554/elife.24550 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 12

Author(s):

Frans Vinberg ◽

Tian Wang ◽

Alicia De Maria ◽

Haiqing Zhao ◽

Steven Bassnett ◽

...

Keyword(s):

Functional Analysis ◽

Temporal Resolution ◽

Light Adaptation ◽

Critical Role ◽

High Temporal Resolution ◽

Deficient Mouse ◽

Operating Range ◽

Response Recovery ◽

Wide Operating

Calcium (Ca2+) plays an important role in the function and health of neurons. In vertebrate cone photoreceptors, Ca2+ controls photoresponse sensitivity, kinetics, and light adaptation. Despite the critical role of Ca2+ in supporting the function and survival of cones, the mechanism for its extrusion from cone outer segments is not well understood. Here, we show that the Na+/Ca2+, K+ exchanger NCKX4 is expressed in zebrafish, mouse, and primate cones. Functional analysis of NCKX4-deficient mouse cones revealed that this exchanger is essential for the wide operating range and high temporal resolution of cone-mediated vision. We show that NCKX4 shapes the cone photoresponse together with the cone-specific NCKX2: NCKX4 acts early to limit response amplitude, while NCKX2 acts late to further accelerate response recovery. The regulation of Ca2+ by NCKX4 in cones is a novel mechanism that supports their ability to function as daytime photoreceptors and promotes their survival.

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Critical Role of the Automodification of Poly(ADP-ribose) Polymerase-1 in Nuclear Factor-κB-dependent Gene Expression in Primary Cultured Mouse Glial Cells

Journal of Biological Chemistry ◽

10.1074/jbc.m407923200 ◽

2004 ◽

Vol 279 (41) ◽

pp. 42774-42786 ◽

Cited By ~ 71

Author(s):

Hidemitsu Nakajima ◽

Hiroshi Nagaso ◽

Nobukazu Kakui ◽

Midori Ishikawa ◽

Toyokazu Hiranuma ◽

...

Keyword(s):

Gene Expression ◽

Glial Cells ◽

Critical Role ◽

Nuclear Factor Κb ◽

Nuclear Factor

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A Glra3 phospho-deficient mouse mutant establishes the critical role of PKA-dependent phosphorylation and inhibition of glycine receptors in spinal inflammatory hyperalgesia

Pain ◽

10.1097/j.pain.0000000000002236 ◽

2021 ◽

Vol Publish Ahead of Print ◽

Author(s):

Karolina Werynska ◽

Jacinthe Gingras ◽

Dietmar Benke ◽

Louis Scheurer ◽

Elena Neumann ◽

...

Keyword(s):

Critical Role ◽

Deficient Mouse ◽

Mouse Mutant ◽

Glycine Receptors ◽

Inflammatory Hyperalgesia

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The Role of the SLP in Autism Spectrum Disorder Screening and Assessment

Perspectives on Language Learning and Education ◽

10.1044/lle15.2.50 ◽

2008 ◽

Vol 15 (2) ◽

pp. 50-59 ◽

Cited By ~ 1

Author(s):

Amy Philofsky

Keyword(s):

Language Development ◽

Critical Role ◽

Children With Autism ◽

Autism Spectrum ◽

Children With Asd ◽

Prevalence Estimates ◽

Notable Increase ◽

Screening Assessment ◽

Critical Components

AbstractRecent prevalence estimates for autism have been alarming as a function of the notable increase. Speech-language pathologists play a critical role in screening, assessment and intervention for children with autism. This article reviews signs that may be indicative of autism at different stages of language development, and discusses the importance of several psychometric properties—sensitivity and specificity—in utilizing screening measures for children with autism. Critical components of assessment for children with autism are reviewed. This article concludes with examples of intervention targets for children with ASD at various levels of language development.

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