Preparation and characterization of PEGDE crosslinked silk fibroin film

2014 ◽  
Vol 29 (5) ◽  
pp. 1083-1089 ◽  
Author(s):  
Yali Wei ◽  
Dan Sun ◽  
Honggen Yi ◽  
Huanrong Zhao ◽  
Jiannan Wang
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroin Film

Preparation and characterization of multilayered hydroxyapatite/silk fibroin film

2007 ◽  
Vol 103 (6) ◽  
pp. 514-520 ◽  
Author(s):  
Rikako Kino ◽  
Toshiyuki Ikoma ◽  
Shunji Yunoki ◽  
Nobuhiro Nagai ◽  
Junzo Tanaka ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroin Film

Characterization of Angiogenesis during Skin Wound Repair by Porous Silk Fibroin Film

2011 ◽  
Vol 175-176 ◽  
pp. 181-185 ◽  
Author(s):  
Kui Hua Zhan ◽  
Lun Bai ◽  
Guo Ping Guan ◽  
Hong Qin Dai
Keyword(s):  
Silk Fibroin ◽  
Rapid Growth ◽  
Wound Repair ◽  
Growth Period ◽  
Interface Layer ◽  
Skin Wound ◽  
Material Layer ◽  
Silk Fibroin Film ◽  
Rehabilitation Period

Based on our experimental data of neovascularization of porous silk fibroin films (PSFF)，in this study we characterized the formation of new blood vessel system at different periods. Firstly, we regarded a wound as two sections, namely, an interface layer and a material layer. Angiogenesis of the interface layer underwent three periods that were rehabilitation period of primary vascular plexus (postoperative 1st day-3rd day), rapid growth period (postoperative 3rd day - 7th day) and remodeling period (after the 7th day). In addition, angiogenesis of the material layer underwent initiation period (postoperative 5th day - 7th day), rapid growth period (postoperative 7th day - 13th day) and remodeling period (after the 13th day). According to the analysis results for angiogenesis, we realized that the efficacy of PSFF on repairing wound was reflected in coordination between infiltration of cells in the pores and angiogenesis, and in timeliness of the newly formed blood vessels’ extention from the interface layer to the material layer.

Preparation and Characterization of Silk Fibroin/Hydroxyapatite Bilayer Scaffolds

2011 ◽  
Vol 415-417 ◽  
pp. 1810-1815 ◽  
Author(s):  
Jian Bing Liu ◽  
Qiang Tang ◽  
Shen Zhou Lu ◽  
Ceng Zhang ◽  
Ming Zhong Li
Keyword(s):  
Silk Fibroin ◽  
Electron Excitation ◽  
Mouse Bone Marrow ◽  
High Porosity ◽  
X Ray Diffraction ◽  
Tissue Engineering Scaffolds ◽  
Molding Method ◽  
Isostatic Compaction ◽  
Fibroin Solution

When the articular cartilage defect accompanies with the subchondral bone defect, using bilayer scaffolds which can integrate with surrounding host cartilage and bone tissue respectively as the tissue engineering scaffolds will be conducive to the repair of tissue defects. This paper reports a new method for preparing bilayer scaffolds. Firstly, hydroxyapatite (HA)/silk fibroin(SF) composite porous materials which have high porosity were prepared by a isostatic compaction molding method, then it was fully immersed in silk fibroin solution, and finally SF/HA bilayer scaffolds were obtained by freeze-drying. The structure of the bilayer scaffolds were investigated through scanning electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, electron excitation spectroscopy and electron microprobe. The results indicated that the upper layer of SF/HA bilayer scaffolds is porous SF component, the under layer is the porous HA/SF composite component and the interface of the two layer is closely connected. Furthermore, mesenchymal stem cells from mouse bone marrow were seeded into the bilayer scaffolds and the results showed that the cells had a well adhesion and growth after culturing for 3 days.

Preparation and characterization of silk fibroin/strontium carbonate film through rapid formation

2017 ◽  
Vol 189 ◽  
pp. 46-49 ◽  
Author(s):  
Min Liu ◽  
Feng Zhang ◽  
Peng Wang ◽  
Jinfa Ming ◽  
Baoqi Zuo
Keyword(s):  
Silk Fibroin ◽  
Strontium Carbonate ◽  
Rapid Formation ◽  
Carbonate Film

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

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