Characterization of Angiogenesis during Skin Wound Repair by Porous Silk Fibroin Film

Based on our experimental data of neovascularization of porous silk fibroin films (PSFF)，in this study we characterized the formation of new blood vessel system at different periods. Firstly, we regarded a wound as two sections, namely, an interface layer and a material layer. Angiogenesis of the interface layer underwent three periods that were rehabilitation period of primary vascular plexus (postoperative 1st day-3rd day), rapid growth period (postoperative 3rd day - 7th day) and remodeling period (after the 7th day). In addition, angiogenesis of the material layer underwent initiation period (postoperative 5th day - 7th day), rapid growth period (postoperative 7th day - 13th day) and remodeling period (after the 13th day). According to the analysis results for angiogenesis, we realized that the efficacy of PSFF on repairing wound was reflected in coordination between infiltration of cells in the pores and angiogenesis, and in timeliness of the newly formed blood vessels’ extention from the interface layer to the material layer.