Morpho-physiological investigations in transgenic tomato (Solanum lycopersicum L.) over expressing OsRGLP1 gene

2021 ◽  
Author(s):  
Sumera Perveen ◽  
Tehmina Ahsan Khan ◽  
Humaira Shaheen ◽  
Rabia Naz ◽  
Muhammad Zeeshan Hyder ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Transgenic Tomato ◽  
Solanum Lycopersicum L

scholarly journals Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L.) fruit

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 26-31 ◽  
Author(s):  
N.H. Loc ◽  
D.T. Long ◽  
T.-G. Kim ◽  
M.-S. Yang
Keyword(s):  
Escherichia Coli ◽  
Solanum Lycopersicum ◽  
Blot Hybridization ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potential Candidate ◽  
B Subunit ◽  
Solanum Lycopersicum L ◽  
Heat Labile ◽  
Enterotoxin B

We report a feasibility study for expressing the LTB protein (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.). We produced five regenerated plants obtained on the selection medium supplemented with an antibiotic. Stable integrations of the LTB&nbsp;gene into the genome of these plants were confirmed by Southern blot hybridization. Western blot analysis showed that only two of the five T<sub>0 </sub>transgenic tomato plants expressed the pentameric LTB protein in the fruits. An enzyme-linked immunosorbent assay indicated that these two plants synthesized the LTB protein bound specifically to GM1 ganglioside, suggesting that the LTB subunits formed active pentamers. The LTB protein produced in tomatoes can be a potential candidate for inexpensive, safe, and effective plant-based vaccines.

scholarly journals Changes in the structure of the rhizosphere complexes of actinomycetes of transgenic tomato (Solanum lycopersicum L., Solanaceae, Solanales) with the gene Fe-SOD 1.

2016 ◽  
Vol 15 (3) ◽  
pp. 341-351
Author(s):  
И. Г. Широких ◽  
◽  
Я. И. Назарова ◽  
С. Ю. Огородникова ◽  
Е. Н. Баранова ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Transgenic Tomato ◽  
Solanum Lycopersicum L
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