scholarly journals Copper Selenide Nanosnakes: Bovine Serum Albumin-Assisted Room Temperature Controllable Synthesis and Characterization

2010 ◽  
Vol 5 (6) ◽  
pp. 949-956 ◽  
Author(s):  
Peng Huang ◽  
Yifei Kong ◽  
Zhiming Li ◽  
Feng Gao ◽  
Daxiang Cui
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Room Temperature ◽  
Copper Selenide ◽  
Synthesis And Characterization ◽  
Controllable Synthesis

Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

2013 ◽  
Vol 641-642 ◽  
pp. 858-861 ◽  
Author(s):  
Zhi Min Ren ◽  
Xi Nie ◽  
Sheng Shu Ai
Keyword(s):  
Bovine Serum Albumin ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Human Serum ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Room Temperature ◽  
Tween 20 ◽  
Blocking Agents

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

Rapid radioimmunoassay of total urinary estriol.

1976 ◽  
Vol 22 (5) ◽  
pp. 611-615 ◽  
Author(s):  
D W Anderson ◽  
U Goebelsmann
Keyword(s):  
Urinary Tract ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Room Temperature ◽  
Urine Specimen ◽  
Ammonium Sulfate Precipitation ◽  
In Pregnancy

Abstract We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.

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