Rapid radioimmunoassay of total urinary estriol.

1976 ◽  
Vol 22 (5) ◽  
pp. 611-615 ◽  
Author(s):  
D W Anderson ◽  
U Goebelsmann
Keyword(s):  
Urinary Tract ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Room Temperature ◽  
Urine Specimen ◽  
Ammonium Sulfate Precipitation ◽  
In Pregnancy

Abstract We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.

scholarly journals THE NATURE OF ANTIGEN-ANTIBODY COMPLEXES FORMED IN RABBITS DURING AN IMMUNE RESPONSE TO BOVINE SERUM ALBUMIN

1958 ◽  
Vol 107 (5) ◽  
pp. 653-663 ◽  
Author(s):  
William O. Weigle
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Ammonium Sulfate Precipitation ◽  
Antibody Synthesis ◽  
Circulating Antigen ◽  
Antigen Antibody

The immune elimination of soluble BSA, following an intravenous injection, is accompanied by the appearance of circulating antigen-antibody complexes. The pattern of the appearance of circulating antigen-antibody complexes and the immune elimination of antigen probably depends on the amount of antigen injected, the rate of antibody synthesis, and perhaps, the quality of antibody produced. There is no relationship between the I* antigen-antibody complexes detected during the immune response in rabbits by ammonium sulfate precipitation and the material precipitated from immune sera as a result of treatment with alkali. Alkali-precipitable material present in the serum of rabbits at a time when I* antigen is also present contain at most only traces of the antigen.

Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

2013 ◽  
Vol 641-642 ◽  
pp. 858-861 ◽  
Author(s):  
Zhi Min Ren ◽  
Xi Nie ◽  
Sheng Shu Ai
Keyword(s):  
Bovine Serum Albumin ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Human Serum ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Room Temperature ◽  
Tween 20 ◽  
Blocking Agents

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

Facile radioimmunoassay of estradiol 3-sulfate in plasma during pregnancy.

1985 ◽  
Vol 31 (11) ◽  
pp. 1846-1848 ◽  
Author(s):  
T Tanaka ◽  
N Suguro ◽  
A Kubodera
Keyword(s):  
Solvent Extraction ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Pregnant Women ◽  
Bovine Serum ◽  
Globulin Fraction ◽  
Specific Antisera ◽  
Pre Treatment ◽  
Sepharose 4B ◽  
In Pregnancy

Abstract A facile, sensitive radioimmunoassay of estradiol 3-sulfate in pregnancy plasma was established. Highly specific antisera were raised in male rabbits against a conjugate of 6-oxoestradiol 3-sulfate-(O-carboxymethyl)oxime with bovine serum albumin. After purification of the antiserum, we coupled the crude globulin fraction to CNBr-activated Sepharose-4B and used this for direct radioimmunoassay of estradiol 3-sulfate in plasma without sample pre-treatment such as solvent-extraction or chromatography to remove endogenous interferences. Using this assay system, we determined concentrations of estradiol 3-sulfate in plasma from pregnant women. Concentrations of estradiol 3-sulfate in plasma increased in proportion to those of estradiol throughout gestation, but were about twice as high as those of unconjugated estradiol at each week of pregnancy.

Dynamic Susceptibility Investigation of Maghemite Nanoparticles Incorporated in Bovine Serum Albumin Template

2008 ◽  
Vol 8 (5) ◽  
pp. 2684-2687 ◽  
Author(s):  
P. C. Morais ◽  
L. B. Silveira ◽  
A. C. Oliveira ◽  
B. M. Lacava ◽  
A. C. Tedesco ◽  
...  
Keyword(s):  
Magnetic Susceptibility ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Energy Barrier ◽  
Room Temperature ◽  
Average Diameter ◽  
Dynamic Susceptibility ◽  
Surface Anisotropy ◽  
Maghemite Nanoparticles

Room-temperature measurements of the magnetic susceptibility of Bovine Serum Albumin-based nanocapsules (50 to 300 nm in size) loaded with different amounts of maghemite nanoparticles (7.6 nm average diameter) have been carried out in this study. The field (H) dependence of the imaginary peak susceptibility (fP) of the nanocomposite samples was investigated in the range of 0 to 4 kOe. From the analysis of the fP × H curves the concentration (N) dependence of the effective maghemite magnetocrystalline energy barrier (E) was obtained. Analysis of the E × N data was performed using a modified Mørup-Tronc [Phys. Rev. Lett. 72, 3278 (1994)] model, from which a huge contribution from the magnetocrystalline surface anisotropy was observed.

Bio-Inspired Synthesis of Bovine Serum Albumin-Conjugated Ag2Se/Se Core/Shell Heterostructure Nanoparticles at Room Temperature

2010 ◽  
Vol 6 (5) ◽  
pp. 446-451 ◽  
Author(s):  
Yi-fei Kong ◽  
Feng Gao ◽  
Rong He ◽  
Jun Chen ◽  
Xin Xu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Room Temperature ◽  
Core Shell

Facilitation of Cleaning of Alumina Surfaces Fouled with Heat-Treated Bovine Serum Albumin by Ozone Treatment

2001 ◽  
Vol 64 (1) ◽  
pp. 108-112 ◽  
Author(s):  
HIROMI URANO ◽  
SATOSHI FUKUZAKI
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Room Temperature ◽  
Treatment Time ◽  
Ozone Treatment ◽  
Gaseous Ozone ◽  
Heat Treated ◽  
Ozone Pretreatment ◽  
Increasing Temperature

Facilitation of cleaning of alumina (Al2O3) particles fouled with heat-treated bovine serum albumin (BSA), which contains sulfhydryl groups on the molecule, by gaseous ozone was studied. With increasing temperature of heat treatment, the amount of adsorbed BSA onto Al2O3 surfaces increased, whereas the rate of BSA desorption during alkali cleaning decreased markedly, resulting in the larger amounts of BSA remaining on Al2O3 surfaces. No significant amounts of BSA were removed from Al2O3 surfaces by alkali cleaning alone when treated at temperatures above 120°C. Before alkali cleaning, the heat-treated, BSA-fouled Al2O3 at 150°C were treated with 0.05 to 0.30% (vol/vol) gaseous ozone at room temperature. Ozone pretreatment markedly accelerated the rate of BSA desorption during subsequent alkali cleaning. The effect of ozone pretreatment on BSA removal depended on the concentration of ozone and treatment time and hence on the total amount of ozone supplied. The molecular weight (MW) of desorbed BSA during alkali cleaning without ozone pretreatment coincided with the MW of the native BSA, whereas the MW of desorbed BSA during the combined ozone-alkali cleaning was lower than the MW of the native BSA. This indicated that the heat-treated BSA molecules adsorbed on Al2O3 were partially decomposed into some fragments by ozone pretreatment, resulting in the facilitation of the removal of BSA during alkali cleaning.

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