An electrochemical biosensor for rapid detection of bovine serum albumin damage induced by hydroxyl radicals in room temperature ionic liquid

2013 ◽  
Vol 188 ◽  
pp. 741-746 ◽  
Author(s):  
Yumin Wang ◽  
Huayu Xiong ◽  
Xiuhua Zhang ◽  
Haoshuang Gu ◽  
Shengfu Wang
Keyword(s):  
Ionic Liquid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Hydroxyl Radicals ◽  
Bovine Serum ◽  
Rapid Detection ◽  
Room Temperature ◽  
Electrochemical Biosensor ◽  
Room Temperature Ionic Liquid

scholarly journals Non-covalent loading of ionic liquid-functionalized nanoparticles for bovine serum albumin: experiments and theoretical analysis

RSC Advances ◽  
2019 ◽  
Vol 9 (33) ◽  
pp. 19114-19120 ◽  
Author(s):  
Xingang Jia ◽  
Xiaoling Hu ◽  
Wenzhen Wang ◽  
Chunbao Du
Keyword(s):  
Ionic Liquid ◽  
Bovine Serum Albumin ◽  
Theoretical Analysis ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Theoretical Calculations ◽  
Covalent Binding ◽  
Functionalized Nanoparticles

Non-covalent binding between nanosilica and bovine serum albumin has been illustrated by experiments and theoretical calculations.

Spectroscopic and docking studies on the interaction between pyrrolidinium based ionic liquid and bovine serum albumin

2014 ◽  
Vol 124 ◽  
pp. 349-356 ◽  
Author(s):  
Meena Kumari ◽  
Jitendra Kumar Maurya ◽  
Upendra Kumar Singh ◽  
Abbul Bashar Khan ◽  
Maroof Ali ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Docking Studies

Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

2013 ◽  
Vol 641-642 ◽  
pp. 858-861 ◽  
Author(s):  
Zhi Min Ren ◽  
Xi Nie ◽  
Sheng Shu Ai
Keyword(s):  
Bovine Serum Albumin ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Human Serum ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Room Temperature ◽  
Tween 20 ◽  
Blocking Agents

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

Rapid radioimmunoassay of total urinary estriol.

1976 ◽  
Vol 22 (5) ◽  
pp. 611-615 ◽  
Author(s):  
D W Anderson ◽  
U Goebelsmann
Keyword(s):  
Urinary Tract ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ammonium Sulfate ◽  
Bovine Serum ◽  
Room Temperature ◽  
Urine Specimen ◽  
Ammonium Sulfate Precipitation ◽  
In Pregnancy

Abstract We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.

Sign in / Sign up

Export Citation Format

Share Document