A novel high frequency SAWR based sensor combined with living cells for shellfish toxin quantitative determination

2021 ◽  
Author(s):  
Liu Wenjia ◽  
Chen Jiasheng ◽  
Pan Weicong ◽  
Dong Yixue ◽  
Ying Xiaoguo
Keyword(s):  
Quantitative Determination ◽  
High Frequency ◽  
Living Cells ◽  
Shellfish Toxin

scholarly journals polo, a mitotic mutant of Drosophila displaying abnormal spindle poles

1988 ◽  
Vol 89 (1) ◽  
pp. 25-38 ◽  
Author(s):  
C.E. Sunkel ◽  
D.M. Glover
Keyword(s):  
High Frequency ◽  
Sex Chromosome ◽  
Living Cells ◽  
Male Meiosis ◽  
Mitotic Spindles ◽  
Spindle Poles ◽  
Immunofluorescence Studies ◽  
Meiotic Spindles ◽  
Circular Arrangement ◽  
Chromosome Disjunction

Neuroblast cells in larvae homozygous for mutant alleles of the locus polo show a high frequency of metaphases in which the chromosomes have a circular arrangement, and anaphase figures in which chromosomes appear to be randomly oriented with respect to at least one of the spindle poles. These defects appear to lead to the production of polyploid cells. Sex chromosome disjunction is affected in male meiosis, primarily in the second division, and the meiotic spindles of living cells are abnormal. One allele is a larval lethal, whereas another is semi-lethal with about 7% of homozygotes surviving as adults. Embryos from homozygous polo females have aberrant mitotic spindles that are highly branched and have broad poles. Immunofluorescence studies with an antibody that recognizes an antigen associated with the centrosome indicate that the organization of this organelle is disrupted in the mutant embryos.

scholarly journals HIGH SPEED PHOTOMICROGRAPHY OF LIVING CELLS SUBJECTED TO SUPERSONIC VIBRATIONS

1931 ◽  
Vol 15 (2) ◽  
pp. 147-153 ◽  
Author(s):  
E. Newton Harvey ◽  
Alfred L. Loomis
Keyword(s):  
High Frequency ◽  
High Speed ◽  
Living Cells ◽  
Air Bubbles ◽  
Sound Waves ◽  
Camera System ◽  
Fluid Movement ◽  
High Frequency Sound ◽  
Frequency Sound ◽  
New Type

A new type of camera system is described capable of taking 1200 pictures a second through a microscope objective. Photographs showing the destruction of Arbacia eggs by high frequency sound waves indicate that the disintegration occurs in less than 1/1200 second. Eggs drawn out into spindle or tadpole shapes suggest that rapid movements of the fluid tearing the eggs may be responsible for the disintegration. Although no cavitated air bubbles show in the photographs, other experiments make it likely that the rapid fluid movement is the result of submicroscopic cavitation.

scholarly journals AN EXPERIMENTAL STUDY OF DIATHERMY

1928 ◽  
Vol 48 (2) ◽  
pp. 235-246 ◽  
Author(s):  
Ronald V. Christie
Keyword(s):  
Experimental Study ◽  
Heat Production ◽  
Small Proportion ◽  
High Frequency ◽  
Biological Material ◽  
Sine Wave ◽  
Living Cells ◽  
Wave Form ◽  
Electrical Measurements ◽  
Appreciable Increase

1. A method is described for measuring the relative impedance of living cells to diathermy currents. 2. The diathermy current penetrates the living cell, and heat production is intracellular as well as extracellular. 3. A small proportion of the impedance of living cells to the diathermy current seems not to lead to the production of heat. 4. Evidence is given that the addition of saponin produces an appreciable increase in the conductivity of an electrolyte. Its use is therefore contraindicated when electrical measurements are being made on biological material. 5. The currents used in diathermy behave as do high frequency currents of the pure sine wave form in respect to their passage through biological material.

scholarly journals High Frequency Microrheology of Living Cells

2016 ◽  
Vol 110 (3) ◽  
pp. 132a ◽  
Author(s):  
Felix Rico ◽  
Annafrancesca Rigato ◽  
Simon Scheuring
Keyword(s):  
High Frequency ◽  
Living Cells

scholarly journals Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution

2010 ◽  
Vol 24 (3-4) ◽  
pp. 303-307 ◽  
Author(s):  
Petr Praus ◽  
Eva Kocišová ◽  
Peter Mojzeš ◽  
Josef Štepánek ◽  
Franck Sureau ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Decay Time ◽  
Spectral Region ◽  
High Frequency ◽  
Antisense Oligonucleotide ◽  
Drug Distribution ◽  
Image Intensifier ◽  
Living Cells ◽  
Time Data ◽  
Time Resolved

Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.

scholarly journals High-frequency microrheology reveals cytoskeleton dynamics in living cells

2017 ◽  
Vol 13 (8) ◽  
pp. 771-775 ◽  
Author(s):  
Annafrancesca Rigato ◽  
Atsushi Miyagi ◽  
Simon Scheuring ◽  
Felix Rico
Keyword(s):  
High Frequency ◽  
Living Cells ◽  
Cytoskeleton Dynamics

Quantitative determination of optical trapping strength and viscoelastic moduli inside living cells

2013 ◽  
Vol 10 (4) ◽  
pp. 046006 ◽  
Author(s):  
Josep Mas ◽  
Andrew C Richardson ◽  
S Nader S Reihani ◽  
Lene B Oddershede ◽  
Kirstine Berg-Sørensen
Keyword(s):  
Quantitative Determination ◽  
Optical Trapping ◽  
Living Cells ◽  
Viscoelastic Moduli
Sign in / Sign up

Export Citation Format

Share Document