Recent advances in small molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live cells and in vivo

2021 ◽  
Author(s):  
Yongqing Zhou ◽  
Xin Wang ◽  
Wei Zhang ◽  
Bo Tang ◽  
Ping Li
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Bioactive Molecules ◽  
Live Cells ◽  
Simultaneous Imaging ◽  
Recent Advances

Simultaneous fluorescence visualization of mitochondrial hydrogen peroxide and zinc ions in live cells and in vivo

2016 ◽  
Vol 52 (86) ◽  
pp. 12741-12744 ◽  
Author(s):  
Haibin Xiao ◽  
Ping Li ◽  
Shan Zhang ◽  
Wei Zhang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Simultaneous Imaging

We have developed two new fluorescent probes termedM-H2O2andM-Znfor simultaneous imaging of hydrogen peroxide and zinc ions in mitochondria.

Recent advances in 1,8-naphthalimide-based small-molecule fluorescent probes for organelles imaging and tracking in living cells

2021 ◽  
Vol 444 ◽  
pp. 214019
Author(s):  
Haitao Yu ◽  
Yan Guo ◽  
Wencheng Zhu ◽  
Kaden Havener ◽  
Xujun Zheng
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Recent Advances

scholarly journals Small-molecule fluorescence-based probes for interrogating major organ diseases

2021 ◽  
Author(s):  
Hai-Hao Han ◽  
He Tian ◽  
Yi Zang ◽  
Adam C. Sedgwick ◽  
Jia Li ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Recent Advances ◽  
Major Organ

This tutorial review describes recent advances involving small molecule fluorescent probes designed to aid in the study of major organ diseases.

scholarly journals Fluorescent Probes with Unnatural Amino Acids to Monitor Proteasome Activity in Real-Time

2020 ◽  
Author(s):  
Breanna L. Zerfas ◽  
Rachel A. Coleman ◽  
Andres Salazar Chaparro ◽  
Nathaniel J. Macatangay ◽  
Darci Trader
Keyword(s):  
Amino Acids ◽  
Small Molecule ◽  
Fluorescent Probes ◽  
Unnatural Amino Acids ◽  
Cell Types ◽  
Proteasome Activity ◽  
Live Cells ◽  
Fluorescent Substrate ◽  
The Individual ◽  
Small Molecule Modulators

<div> <div> <div> <p>The proteasome is an essential protein complex that, when dysregulated, can result in various diseases in eukaryotic cells. As such, understanding the enzymatic activity of the proteasome and what can alter it is crucial to elucidating its roles in these diseases. This can be done effectively by using activity-based fluorescent substrate probes, of which there are many commercially available that target the individual protease-like subunits in the 20S CP of the proteasome. Unfortunately, these probes have not displayed appropriate characteristics for their use in live cell-based assays. In the work presented here, we have developed a set of probes which have shown improved fluorescence properties and selectivity towards the proteasome compared to other cellular proteases. By including unnatural amino acids, we have found probes which can be utilized in various applications, including monitoring the effects of small molecule stimulators of the proteasome in live cells and comparing the relative proteasome activity across different cancer cell types. In future studies, we expect the fluorescent probes presented here will serve as tools to support the discovery and characterization of small molecule modulators of proteasome activity. </p> </div> </div> </div>

scholarly journals Recent Advances in Understanding and Engineering Polyketide Synthesis

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 208 ◽  
Author(s):  
Wenjun Zhang ◽  
Joyce Liu
Keyword(s):  
Biochemical Analysis ◽  
Polyketide Synthase ◽  
Bioactive Molecules ◽  
New Developments ◽  
Recent Advances ◽  
Fundamental Understanding ◽  
Block Formation ◽  
Enzymatic Machinery

Polyketides are a diverse group of natural products that form the basis of many important drugs. The engineering of the polyketide synthase (PKS) enzymes responsible for the formation of these compounds has long been considered to have great potential for producing new bioactive molecules. Recent advances in this field have contributed to the understanding of this powerful and complex enzymatic machinery, particularly with regard to domain activity and engineering, unique building block formation and incorporation, and programming rules and limitations. New developments in tools for in vitro biochemical analysis, full-length megasynthase structural studies, and in vivo heterologous expression will continue to improve our fundamental understanding of polyketide synthesis as well as our ability to engineer the production of polyketides.

Recent advances in stimuli-responsive in situ self-assembly of small molecule probes for in vivo imaging of enzymatic activity

2021 ◽  
Author(s):  
Yuqi Wang ◽  
Jianhui Weng ◽  
Xidan Wen ◽  
Yuxuan Hu ◽  
Deju Ye
Keyword(s):  
Enzymatic Activity ◽  
Small Molecule ◽  
Self Assembly ◽  
In Vivo Imaging ◽  
Molecular Probes ◽  
Stimuli Responsive ◽  
Small Molecule Probes ◽  
Recent Advances

Stimuli-responsive in situ self-assembly of small molecule probes into nanostructures has been promising for the construction of molecular probes for in vivo imaging.

Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging

2018 ◽  
Vol 47 (18) ◽  
pp. 7140-7180 ◽  
Author(s):  
Hong-Wen Liu ◽  
Lanlan Chen ◽  
Chengyan Xu ◽  
Zhe Li ◽  
Haiyang Zhang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Cancer Imaging ◽  
Design Strategies ◽  
Recent Advances

An overview of recent advances in small-molecule enzymatic fluorescent probes for cancer imaging, including design strategies and cancer imaging applications.

Recent advances in mitochondria- and lysosomes-targeted small-molecule two-photon fluorescent probes

2017 ◽  
Vol 28 (10) ◽  
pp. 1943-1951 ◽  
Author(s):  
Peng Ning ◽  
Wenjuan Wang ◽  
Man Chen ◽  
Yan Feng ◽  
Xiangming Meng
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Two Photon ◽  
Recent Advances

Recent Advances in Fluorescence Imaging of Bioactive Molecules in Neurons and in Vivo

2019 ◽  
Vol 47 (10) ◽  
pp. 1537-1548 ◽  
Author(s):  
Xin WANG ◽  
Ping LI ◽  
Wen ZHANG ◽  
Bo TANG
Keyword(s):  
Fluorescence Imaging ◽  
Bioactive Molecules ◽  
Recent Advances
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