The transcription factor PjERF1 enhances the biosynthesis of triterpenoid saponins in Panax japonicus

2021 ◽  
Author(s):  
Qin Chen ◽  
Yilin Yu ◽  
Xiang Zhang ◽  
Ren Zhao ◽  
Jinyu Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Triterpenoid Saponins ◽  
Panax Japonicus

scholarly journals New Triterpenoid Saponins from Leaves of Panax Japonicus (3). Saponins of the Specimens Collected in Miyazaki Prefecture

2012 ◽  
Vol 7 (4) ◽  
pp. 1934578X1200700
Author(s):  
Kouichi Yoshizaki ◽  
Shoji Yahara
Keyword(s):  
Ginsenoside Re ◽  
Triterpenoid Saponins ◽  
Panax Japonicus

Two new dammarane-type triterpenoid saponins, chikusetsusaponin LM1 (1), chikusetsusaponin LM2 (2), and three known triterpenoid saponins, ginsenoside Re (3), ginsenoside Rg1 (4), ginsenoside F3 (5), were isolated from the leaves of P. japonicus C. A. Meyer collected in Miyazaki prefecture, Japan. The structures of new chikusetsusaponins were elucidated on the basis of spectroscopic and physicochemical evidences.

Triterpenoid Saponins from Two Panax japonicus Varietals Used in Tujia Ethnomedicine

2015 ◽  
Vol 1 (2) ◽  
pp. 122-135 ◽  
Author(s):  
Yang Liu ◽  
Michael Chan ◽  
Jianhua Huang ◽  
Bin Li ◽  
Wen Ouyang ◽  
...  
Keyword(s):  
Triterpenoid Saponins ◽  
Panax Japonicus

scholarly journals New Triterpenoid Saponins from Fruit Specimens of Panax japonicus Collected in Toyama Prefecture and Hokkaido (2)

2012 ◽  
Vol 60 (6) ◽  
pp. 728-735 ◽  
Author(s):  
Kouichi Yoshizaki ◽  
Morikazu Murakami ◽  
Hiroharu Fujino ◽  
Naotoshi Yoshida ◽  
Shoji Yahara
Keyword(s):  
Triterpenoid Saponins ◽  
Panax Japonicus

scholarly journals Four New Triterpenoid Saponins from the Leaves of Panax japonicus Grown in Southern Miyazaki Prefecture (4)

2013 ◽  
Vol 61 (3) ◽  
pp. 273-278 ◽  
Author(s):  
Kouichi Yoshizaki ◽  
Hari Prasad Devkota ◽  
Shoji Yahara
Keyword(s):  
Triterpenoid Saponins ◽  
Panax Japonicus

scholarly journals Transcriptomic Analysis Reveals the Mechanism of Glycyrrhizic acid Biosynthesis in Glycyrrhiza Uralensis Fisch.

2021 ◽  
Author(s):  
Junping He ◽  
Lu Yao ◽  
Juan Wang ◽  
Wenyuan Gao
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Glycyrrhizic Acid ◽  
Average Length ◽  
Glycyrrhetinic Acid ◽  
Glycyrrhiza Uralensis ◽  
Amino Acid Sequence Similarity ◽  
Acid Biosynthesis ◽  
Triterpenoid Saponins ◽  
High Amino Acid

Abstract In order to better understand the mechanism of glycyrrhizic acid biosynthesis and explore important enzyme gene resources in Glycyrrhiza uralensis Fisch., we sequenced the transcriptome of the adventitious roots of G. uralensis treated by methyl jasmonate (MJ) and assembled the de novo sequence. 256503 unique transcripts with an average length of 898bp were produced. Transcriptome sequencing and data analysis showed that the key genes of glycyrrhizic acid biosynthesis changed significantly after MJ treatment. 2720 up-regulated genes and 3493 down regulated genes were found. In the process of oxidation and glycosylation of glycyrrhizic acid biosynthesis. A putative CYP450 gene (Cluster-30944.70498) is positively correlated with glycyrrhetinic acid. The glycosyltransferase gene (Cluster-30944.25725) is positively correlated with glycyrrhizic acid and glycyrrhetinic acid. In addition, we found an AP2-EREBP family transcription factor (Cluster-30944.55070). It had high amino acid sequence similarity with PgERF1. In Panax ginseng, PgERF1 was identified as promoting the biosynthesis of triterpenoid saponins. According to the correlation analysis of transcription factors, functional gene expression and component accumulation, we speculated that this transcription factor can positively regulate the expression of farnesyl diphosphate, squalene epoxide and glycosyltransferase (Cluster-30944.25725) genes and ultimately increase the content of glycyrrhizic acid.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression
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