Effect of Salicylic Acid on the Activity of PAL and PHB Geranyltransferase and Shikonin Derivatives Production in Cell Suspension Cultures of Arnebia euchroma (Royle) Johnst—a Medicinally Important Plant Species

2014 ◽  
Vol 173 (1) ◽  
pp. 248-258 ◽  
Author(s):  
Pawan Kumar ◽  
Mahak Saini ◽  
Shashi Bhushan ◽  
Ashish R. Warghat ◽  
Tarun Pal ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Cell Suspension ◽  
Plant Species ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Arnebia Euchroma ◽  
Shikonin Derivatives

scholarly journals Liquid Perfluorodecalin Application for In Situ Extraction and Enhanced Naphthoquinones Production in Arnebia euchroma Cell Suspension Cultures

2014 ◽  
Vol 172 (5) ◽  
pp. 2618-2627 ◽  
Author(s):  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek ◽  
Michał Cichosz ◽  
Agnieszka Pietrosiuk
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
In Situ Extraction ◽  
Arnebia Euchroma

Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon

1987 ◽  
Vol 6 (3) ◽  
pp. 197-199 ◽  
Author(s):  
Hiroshi Deno ◽  
Chuzo Suga ◽  
Teijiro Morimoto ◽  
Yasuhiro Fujita
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Lithospermum Erythrorhizon ◽  
Shikonin Derivatives

Über den Abbau von Flavonolen in pflanzlichen Zellsuspensionskulturen / Degradation of Flavonols in Plant Suspension Cultures

1972 ◽  
Vol 27 (8) ◽  
pp. 946-954 ◽  
Author(s):  
Wolfgang Hösel ◽  
Paul D. Shaw ◽  
Wolfgang Barz
Keyword(s):  
Salicylic Acid ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Enzyme Preparations ◽  
Cicer Arietinum L

The flavonols kaempferol, quercetin and isorhamnetin were labelled with 14C by keeping seven day old Cicer arietinum L. plants in an atmosphere of 14CO2 for five days. The purified (U-14C) flavonols were applied to cell suspension cultures of Cicer arietinum L., Phaseolus aureus Roxb., Glycine max and Petroselinum hortense. Based on the rates of 14CO2 formation and distribution of radioactivity after fractionation of the cells, the flavonols were shown to be catabolized to a very high extent.All four cell suspension cultures possess the enzymatic activity transforming flavonols to the recently discovered 2,3-dihydroxyflavanones. Upon incubation of the flavonols datiscetin and kaempferol with enzyme preparations from Cicer arietinum L. cell suspension cultures, it was demonstrated that the enzymatically formed 2,3-dihydroxyflavanones are further transformed in an enzyme catalyzed reaction. Salicylic acid was found as a degradation fragment of ring B of the 2,3,5,7,2′-pentahydroxyflavanone derived from datiscetin. Neither phloroglucinol nor phloroglucinol carboxylic acid were observed as metabolites of ring A. These in vitro findings were further substantiated by in vivo data because the flavonols kaempferol, quercetin and datiscetin when applied to cell suspension cultures of Cicer arietinum L. and Glycine max gave rise to para-hydroxybenzoic acid, protocatechuic acid and salicylic acid, respectively. It was thus concluded that flavonols are catabolized via 2,3-dihydroxyflavanones with the B-ring liberated as the respective benzoic acid. The data are discussed in connection with earlier findings on the catabolism of chalcones, cinnamic and benzoic acids.

scholarly journals Dicentrine Production in Callus and Cell Suspension Cultures of Stephania Venosa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tharita Kitisripanya ◽  
Jukrapun Komaikul ◽  
Nirachara Tawinkan ◽  
Chuennapha Atsawinkowit ◽  
Waraporn Putalun
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Alternative Source ◽  
Plant Materials ◽  
Stem Segments

The highest dicentrine content (19.5 ± 0.3 mg/g dry weight) from callus culture of Stephania venosa was achieved from stem segments cultured on MS medium supplemented with TDZ 0.5 mg/L and NAA 1.0 mg/L. Cell suspension cultures were established from callus cultured on MS liquid medium with the same plant growth regulators. Dicentrine production from S. venosa cell suspension cultures was obtained in the range of 15–26 mg/g dry weight. Elicitation in cell suspension cultures by chitosan (50 mg/L) and salicylic acid (2 mg/L) for 6 days significantly increased dicentrine content. Our findings indicate that callus and cell suspension cultures of S. venosa can produce high levels of dicentrine as an alternative source of plant materials.

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