scholarly journals Salicylic acid mediated up regulation of carvone biosynthesis during growth phase in cell suspension cultures of Anethum graveolens

3 Biotech ◽  
2020 ◽  
Vol 10 (11) ◽  
Author(s):  
Neha Bulchandani ◽  
Gyan S. Shekhawat
Keyword(s):  
Salicylic Acid ◽  
Cell Suspension ◽  
Growth Phase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Anethum Graveolens

Über den Abbau von Flavonolen in pflanzlichen Zellsuspensionskulturen / Degradation of Flavonols in Plant Suspension Cultures

1972 ◽  
Vol 27 (8) ◽  
pp. 946-954 ◽  
Author(s):  
Wolfgang Hösel ◽  
Paul D. Shaw ◽  
Wolfgang Barz
Keyword(s):  
Salicylic Acid ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Enzyme Preparations ◽  
Cicer Arietinum L

The flavonols kaempferol, quercetin and isorhamnetin were labelled with 14C by keeping seven day old Cicer arietinum L. plants in an atmosphere of 14CO2 for five days. The purified (U-14C) flavonols were applied to cell suspension cultures of Cicer arietinum L., Phaseolus aureus Roxb., Glycine max and Petroselinum hortense. Based on the rates of 14CO2 formation and distribution of radioactivity after fractionation of the cells, the flavonols were shown to be catabolized to a very high extent.All four cell suspension cultures possess the enzymatic activity transforming flavonols to the recently discovered 2,3-dihydroxyflavanones. Upon incubation of the flavonols datiscetin and kaempferol with enzyme preparations from Cicer arietinum L. cell suspension cultures, it was demonstrated that the enzymatically formed 2,3-dihydroxyflavanones are further transformed in an enzyme catalyzed reaction. Salicylic acid was found as a degradation fragment of ring B of the 2,3,5,7,2′-pentahydroxyflavanone derived from datiscetin. Neither phloroglucinol nor phloroglucinol carboxylic acid were observed as metabolites of ring A. These in vitro findings were further substantiated by in vivo data because the flavonols kaempferol, quercetin and datiscetin when applied to cell suspension cultures of Cicer arietinum L. and Glycine max gave rise to para-hydroxybenzoic acid, protocatechuic acid and salicylic acid, respectively. It was thus concluded that flavonols are catabolized via 2,3-dihydroxyflavanones with the B-ring liberated as the respective benzoic acid. The data are discussed in connection with earlier findings on the catabolism of chalcones, cinnamic and benzoic acids.

scholarly journals Dicentrine Production in Callus and Cell Suspension Cultures of Stephania Venosa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tharita Kitisripanya ◽  
Jukrapun Komaikul ◽  
Nirachara Tawinkan ◽  
Chuennapha Atsawinkowit ◽  
Waraporn Putalun
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Alternative Source ◽  
Plant Materials ◽  
Stem Segments

The highest dicentrine content (19.5 ± 0.3 mg/g dry weight) from callus culture of Stephania venosa was achieved from stem segments cultured on MS medium supplemented with TDZ 0.5 mg/L and NAA 1.0 mg/L. Cell suspension cultures were established from callus cultured on MS liquid medium with the same plant growth regulators. Dicentrine production from S. venosa cell suspension cultures was obtained in the range of 15–26 mg/g dry weight. Elicitation in cell suspension cultures by chitosan (50 mg/L) and salicylic acid (2 mg/L) for 6 days significantly increased dicentrine content. Our findings indicate that callus and cell suspension cultures of S. venosa can produce high levels of dicentrine as an alternative source of plant materials.

scholarly journals Metabolic changes of salicylic acid-elicited Catharanthus roseus cell suspension cultures monitored by NMR-based metabolomics

2009 ◽  
Vol 31 (12) ◽  
pp. 1967-1974 ◽  
Author(s):  
Natali Rianika Mustafa ◽  
Hye Kyong Kim ◽  
Young Hae Choi ◽  
Robert Verpoorte
Keyword(s):  
Salicylic Acid ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Metabolic Changes

scholarly journals A Study On The Effect of Different Elicitors On Capsaicin Accumulation in Cell Suspension Cultures of Capsicum Assamicum (Bhut Jolokia)

2021 ◽  
Author(s):  
Swet Nisha ◽  
Ajitabh Bora ◽  
HK Gogoi ◽  
SK Dwivedi ◽  
PJ Handique
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Sinapic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Exponential Phase ◽  
Bhut Jolokia

Abstract Elicitation of cell suspension cultures of Capsicum assamicum (Bhut Jolokia) for enhancement of capsaicin content was tried using different elicitors such as cellulase, vanillin, methyl jasmonate, salicylic acid and sinapic acid in different concentrations for 24, 48 and 72 hours. Cell suspension culture was established in B5 media supplemented with 3.5 mM 2,4-D (2,4-diphenoxyacetic acid) and 1.1 mM Kin and elicitors were introduced at the end of exponential phase. All the elicitors, except methyl jasmonate, led to significant increase in production of capsaicin. Sinapic acid, when added in 22 µM concentration and incubated for 24 hours, led to highest capsaicin accumulation of 0.5% (5068 µg/g) which was highest among all the treatments.

Glucosylation of salicylic acid by cell suspension cultures of Mallotus japonicus

1990 ◽  
Vol 9 (6) ◽  
Author(s):  
Y. Umetani ◽  
E. Kodakari ◽  
T. Yamamura ◽  
S. Tanaka ◽  
M. Tabata
Keyword(s):  
Salicylic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Mallotus Japonicus
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