Use of Crude Glycerol as Sole Carbon Source for Microbial Lipid Production by Oleaginous Yeasts

2016 ◽  
Vol 182 (2) ◽  
pp. 495-510 ◽  
Author(s):  
Li-ping Liu ◽  
Yang Hu ◽  
Wen-yong Lou ◽  
Ning Li ◽  
Hong Wu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Crude Glycerol ◽  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Microbial lipid production from pectin-derived carbohydrates by oleaginous yeasts

2015 ◽  
Vol 50 (7) ◽  
pp. 1097-1102 ◽  
Author(s):  
Yandan Wang ◽  
Zhiwei Gong ◽  
Xiaobing Yang ◽  
Hongwei Shen ◽  
Qian Wang ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Recycling microbial lipid production wastes to cultivate oleaginous yeasts

2015 ◽  
Vol 175 ◽  
pp. 91-96 ◽  
Author(s):  
Xiaobing Yang ◽  
Guojie Jin ◽  
Zhiwei Gong ◽  
Hongwei Shen ◽  
Fengwu Bai ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Production Wastes ◽  
Microbial Lipid Production

scholarly journals Volatile Fatty Acids from Lipid-Extracted Yeast Provide Additional Feedstock for Microbial Lipid Production

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1009
Author(s):  
Gwon Woo Park ◽  
Seongsoo Son ◽  
Myounghoon Moon ◽  
Subin Sin ◽  
Kyoungseon Min ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Response Surface Methodology ◽  
Carbon Source ◽  
Response Surface ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Initial Ph ◽  
Microbial Lipid Production

Microbial lipid production from oleaginous yeasts is a promising process for the sustainable development of the microbial biodiesel industry. However, the feedstock cost poses an economic problem for the production of microbial biodiesel. After lipid extraction, yeast biomass can be used as an organic source for microbial biodiesel production. In this study, volatile fatty acids (VFAs), produced via anaerobic digestion of a lipid-extracted yeast (LEY) residue, were utilized as a carbon source for the yeast Cryptococcus curvatus. The response surface methodology was used to determine the initial pH and inoculum volume for the optimal VFA production. The experimental result for VFA concentration was 4.51 g/L at an initial pH of 9 and an inoculation 25%. The optimization results from the response surface methodology showed that the maximal VFA concentration was 4.58 g/L at an initial pH of 8.40 and an inoculation of 39.49%. This study indicates that VFAs from LEY can be used as a carbon source for microbial biodiesel production, with the potential to significantly reduce feedstock costs.

Microbial lipid production by oleaginous yeasts on Laminaria residue hydrolysates

RSC Advances ◽  
2016 ◽  
Vol 6 (32) ◽  
pp. 26752-26756 ◽  
Author(s):  
Xibin Zhang ◽  
Hongwei Shen ◽  
Xiaobing Yang ◽  
Qian Wang ◽  
Xue Yu ◽  
...  
Keyword(s):  
Lipid Production ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Microbial Lipids ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Laminaria residues, major wastes from the kelp industry, can be effectively converted by oleaginous yeasts into microbial lipids as potential feedstock for biodiesel production.

scholarly journals Bio-lipid production from crude glycerol and lignocellulosic hydrolysate with oleaginous yeasts

2020 ◽  
Author(s):  
Mikolaj Chmielarz ◽  
Johanna Blomqvist ◽  
Sabine Sampels ◽  
Mats Sandgren ◽  
Volkmar Passoth
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Paper Industry ◽  
Lipid Production ◽  
Strain Differences ◽  
Hemicellulose Hydrolysate ◽  
Lipid Concentration ◽  
Oleaginous Yeasts ◽  
Glycerol Conversion ◽  
Fermentation Time

Abstract Background: Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low- value side-products of biodiesel transesterification and pulp- and paper industry, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results: Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi , was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi . Two strains - Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 40% HH medium mixed with 60 g/L CG - 19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.22 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions: There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low- value compounds to biolipids.

scholarly journals Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Mikolaj Chmielarz ◽  
Johanna Blomqvist ◽  
Sabine Sampels ◽  
Mats Sandgren ◽  
Volkmar Passoth
Keyword(s):  
Carbon Source ◽  
Crude Glycerol ◽  
Paper Industry ◽  
Lipid Production ◽  
Microbial Lipids ◽  
Hemicellulose Hydrolysate ◽  
Lipid Concentration ◽  
Oleaginous Yeasts ◽  
Glycerol Conversion ◽  
Fermentation Time

Abstract Background Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low—value side-products of biodiesel transesterification and pulp—and paper industry or lignocellulosic ethanol production, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi, was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi. Two strains—Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 10% HH medium mixed with 55 g/L CG—19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.25 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation in R. toruloides and R. glutinis resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low-value compounds to microbial lipids.

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