The Influence of Submerged Fermentation of Inonotus obliquus with Control Atmosphere Treatment on Enhancing Bioactive Ingredient Contents

2020 ◽  
Vol 191 (1) ◽  
pp. 412-425
Author(s):  
Hsin-Jung Chen ◽  
Yuh-Shuen Chen ◽  
Shih-Lun Liu ◽  
Bo-Kang Liou ◽  
Chin-Shuh Chen
Keyword(s):  
Submerged Fermentation ◽  
Control Atmosphere ◽  
Inonotus Obliquus

scholarly journals Effect of Alkali-Treated Birch Sawdust on the Lignocellulase Secretion and Exo-Polysaccharide Production by Inonotus Obliquus Under Submerged Fermentation and its Lignocellulose Degradation Patterns

2021 ◽  
Author(s):  
Jiaming Zhang ◽  
Xiaohong Lu ◽  
Zhezhen Zhao ◽  
Mengya Wang ◽  
Ping Liu
Keyword(s):  
Submerged Fermentation ◽  
Degradation Rate ◽  
Lignocellulose Degradation ◽  
Lignocellulolytic Enzymes ◽  
Inhibition Rate ◽  
Alkali Pretreatment ◽  
Inonotus Obliquus ◽  
Polysaccharide Production ◽  
Degradation Analysis ◽  
Lignocellulase Secretion

Abstract The objectives of this study were to investigate the medicinal mushroom Inonotus obliquus on the production of polysaccharides and changes of extracellular lignocellulolytic enzymes during submerged fermentation using alkali-treated birch sawdust as substrate. Meanwhile, in order to explore the degradation mode of lignocellulose in alkali-treated birch sawdust, degradation analysis of three components of lignocellulose was carried out. The fungus process in alkali-treated birch sawdust medium resulted in a higher degradation rate of cellulose, hemicellulose, and lignin of 39.24%, 51.00% and 31.3% after 11 days of submerged fermentation by the mycelium of I. obliquus, respectively. Maximal polysaccharide production and α-glucosidase inhibition rate determined in the alkali-treated birch sawdust medium were 6.93 mg/mL and 55.80%,while they were 4.98 mg/mL and 27.89% in the control. Moreover, high activities of Laccase (51.95 IU/mL) , CMCase (1.35 IU/mL) , FPA (0.50 IU/mL) and β-glucosidase (0.55 IU/mL) were observed in alkali-treated birch sawdust medium, respectively. The results demonstrated that the addition of alkali-treated birch sawdust could promote the yield of active polysaccharides and induce the production of cellulase and xylanase, indicating that alkali pretreatment was conducive to utilization of birch sawdust by I. obliquus.

scholarly journals Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4400 ◽  
Author(s):  
Mengya Wang ◽  
Zhezhen Zhao ◽  
Xia Zhou ◽  
Jinrong Hu ◽  
Jiao Xue ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Sugar Content ◽  
Monosaccharide Composition ◽  
Reducing Sugar ◽  
Inhibition Rate ◽  
Mycelial Biomass ◽  
Inonotus Obliquus ◽  
Hypoglycaemic Activity ◽  
Ic50 Value ◽  
Simultaneous Accumulation

This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition. Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus. The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition. The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL. Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively. In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1. Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.

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