The Role of the Biochemical and Biophysical Environment in Chondrogenic Stem Cell Differentiation Assays and Cartilage Tissue Engineering

2008 ◽  
Vol 52 (2) ◽  
pp. 85-102 ◽  
Author(s):  
Kristin E. Wescoe ◽  
Rebecca C. Schugar ◽  
Constance R. Chu ◽  
Bridget M. Deasy
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Cartilage Tissue Engineering ◽  
Stem Cell Differentiation ◽  
Cartilage Tissue ◽  
Biophysical Environment ◽  
And Cartilage

Magnetic field assisted stem cell differentiation – role of substrate magnetization in osteogenesis

2015 ◽  
Vol 3 (16) ◽  
pp. 3150-3168 ◽  
Author(s):  
Sunil Kumar Boda ◽  
Greeshma Thrivikraman ◽  
Bikramjit Basu
Keyword(s):  
Magnetic Field ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Bone Tissue Engineering ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation

Substrate magnetization as a tool for modulating the osteogenesis of human mesenchymal stem cells for bone tissue engineering applications.

scholarly journals Regulation of Redox Homeostasis by Nonthermal Biocompatible Plasma Discharge in Stem Cell Differentiation

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Ying Li ◽  
Eun Ha Choi ◽  
Ihn Han
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Redox Homeostasis ◽  
Stem Cell Differentiation ◽  
Redox Biology ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Recently, a growing body of evidence has shown the role of reactive species as secondary messengers in cell proliferation and differentiation, as opposed to the harmful metabolism byproducts that they were previously solely recognized as. Thus, the balance of intracellular reduction-oxidation (redox) homeostasis plays a vital role in the regulation of stem cell self-renewal and differentiation. Nonthermal biocompatible plasma (NBP) has emerged as a novel tool in biomedical applications. Recently, NBP has also emerged as a powerful tool in the tissue engineering field for the surface modification of biomaterial and the promotion of stem cell differentiation by the regulation of intracellular redox biology. NBP can generate various kinds of reactive oxygen species (ROS) and reactive nitrogen species (RNS), which may play the role of the second passenger in the cell signaling network and active antioxidant system in cells. Herein, we review the current knowledge on mechanisms by which NBP regulates cell proliferation and differentiation through redox modification. Considering the importance of redox homeostasis in the regulation of stem cell differentiation, understanding the underlying molecular mechanisms involved will provide important new insights into NBP-induced stem cell differentiation for tissue engineering.

scholarly journals Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation

2018 ◽  
Author(s):  
Runa Begum ◽  
Adam W. Perriman ◽  
Bo Su ◽  
Fabrizio Scarpa ◽  
Wael Kafienah
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Growth Factor ◽  
Polymer Composite ◽  
Cartilage Tissue Engineering ◽  
Stem Cell Differentiation ◽  
Cartilage Tissue ◽  
Natural Polymer ◽  
Natural Polymers ◽  
Electrospinning Technique

AbstractSmart biomaterials with an inherent stimulating capacity that elicit specific behavioursin lieuof biological prompts would prove advantageous for regenerative medicine applications. Specific blends of the natural polymers cellulose and silk cast as films can drive the chondrogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) uponin vitroculture. However, the true potential of such biomaterials for cartilage tissue engineering can be realised upon its three-dimensional fabrication. In this work we employ an electrospinning technique to model thein vivonanofibrous extracellular matrix (ECM). Cellulose and silk polymers at a mass ratio of 75:25 were regenerated using a trifluoroacetic acid and acetic acid cosolvent system. This natural polymer composite was directly electrospun for the first time, into nanofibers without post-spun treatment. The presence and size of fibre beading was influenced by environmental humidity. The regenerated composite retained the key chemical functionalities of its respective components. Biocompatibility of the natural polymer composite with hMSCs was demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, was confirmed. This physical chondrogenic stimulation was countered biochemically using fibroblast growth factor-2 (FGF-2), a growth factor used to enhance the proliferation of hMSCs. The newly fabricated scaffold provides the foundation for designing a robust, self-inductive, and cost-effective biomimetic biomaterial for cartilage tissue engineering.

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