Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme

2019 ◽  
Vol 15 (4) ◽  
pp. 543-557 ◽  
Author(s):  
Irma Virant-Klun ◽  
S. Omejec ◽  
M. Stimpfel ◽  
P. Skerl ◽  
S. Novakovic ◽  
...  
Keyword(s):  
Stem Cell ◽  
In Vitro Fertilization ◽  
Mesenchymal Stem Cell ◽  
Follicular Cells ◽  
Female Age ◽  
Vitro Fertilization

scholarly journals Investigation of Stem Cell Applications on In Vitro Fertilization in Rats

2021 ◽  
Vol 72 (3) ◽  
pp. 3067
Author(s):  
A GUMURDU ◽  
S OZTURK ◽  
I AYDEMIR ◽  
MI TUGLU
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
In Vitro Fertilization ◽  
Mesenchymal Stem Cell ◽  
Conditioned Media ◽  
Female Rats ◽  
Favourable Effect ◽  
Vitro Fertilization ◽  
Male And Female Rats

We aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryos.

scholarly journals Stem Cells as a Resource for Treatment of Infertility-related Diseases

2019 ◽  
Vol 19 (8) ◽  
pp. 539-546
Author(s):  
Jing Wang ◽  
Chi Liu ◽  
Masayuki Fujino ◽  
Guoqing Tong ◽  
Qinxiu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
In Vitro Fertilization ◽  
Cell Therapy ◽  
Reproductive Medicine ◽  
Intrauterine Insemination ◽  
Reproductive Age ◽  
Vitro Fertilization ◽  
Undifferentiated Cells

Worldwide, infertility affects 8-12% of couples of reproductive age and has become a common problem. There are many ways to treat infertility, including medication, intrauterine insemination, and in vitro fertilization. In recent years, stem-cell therapy has raised new hope in the field of reproductive disability management. Stem cells are self-renewing, self-replicating undifferentiated cells that are capable of producing specialized cells under appropriate conditions. They exist throughout a human’s embryo, fetal, and adult stages and can proliferate into different cells. While many issues remain to be addressed concerning stem cells, stem cells have undeniably opened up new ways to treat infertility. In this review, we describe past, present, and future strategies for the use of stem cells in reproductive medicine.

scholarly journals In vitro Fertilization of Tubal Rabbit Ova Partially or Totally Denuded of Follicular Cells

1971 ◽  
Vol 4 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Lynn R. Fraser ◽  
Pramila V. Dandekar ◽  
Rama A. Vaidya
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Cells ◽  
Vitro Fertilization

Why Scientific Details are Important When Novel Technologies Encounter Law, Politics, and Ethics

2010 ◽  
Vol 38 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Lawrence Goldstein
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
In Vitro Fertilization ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Advanced Technology ◽  
Human Embryos ◽  
Vitro Fertilization ◽  
Novel Technologies

Lost at times in the heat of debate about stem cell research, or any controversial advanced technology, is the need for precision in debate and discussion. The details matter a great deal, ranging from the need to use words that have precise definitions, to accurately quote colleagues and adversaries, and to cite scientific and medical results in a way that reflects the quality, rigor, and reliability of the work at issue. Regrettably, considerable inaccuracy has found its way into the debates about stem cells, on all sides, with consequent fogging of the issues.A key detail that is often overlooked in the debates about the uses of human embryonic stem cells in research comes from the nature of in vitro fertilization (IVF) treatment for infertility. Specifically, there are many frozen human embryos (more precisely called blastocysts) that are in excess of reproductive needs of the couple who generated them, and that must be either frozen indefinitely, donated to another couple, or destroyed.

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