Glioma Cells Promote the Expression of Vascular Cell Adhesion Molecule-1 on Bone Marrow-Derived Mesenchymal Stem Cells: a Possible Mechanism for Their Tropism Toward Gliomas

2012 ◽  
Vol 48 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Yi Hu ◽  
Peng Cheng ◽  
Yi-Xue Xue ◽  
Yun-Hui Liu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Glioma Cells ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Cell Adhesion Molecule 1

scholarly journals Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 388-395 ◽  
Author(s):  
PJ Simmons ◽  
B Masinovsky ◽  
BM Longenecker ◽  
R Berenson ◽  
B Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Marrow Stromal Cells ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Cd34 Cells ◽  
Cell Adhesion Molecule 1

Human bone marrow-derived CD34+ cells were analyzed for the expression of the beta 1-family of integrin adhesion molecules. Integrin alpha 4 beta 1 was consistently expressed by greater than 90% of CD34+ cells, including essentially all assayable granulocyte-macrophage colony- forming cells (CFU-GM) and erythroid bursts (BFU-E) as shown by fluorescence-activated cell sorting studies. Adhesion of highly enriched CD34+ cells to cultured allogeneic marrow stromal cells was largely inhibited both by monoclonal antibody to alpha 4 beta 1 and to vascular cell adhesion molecule-1 (VCAM-1), a ligand for alpha 4 beta 1. VCAM-1 was found to be expressed by bone marrow stromal elements in vitro both constitutively at low level and at high levels after treatment with cytokines. Induction of VCAM-1 was cytokine- and time- dependent with maximum levels being obtained after 4 hours of exposure to a combination of interleukin-4 and tumor necrosis factor-alpha. Cytokine-induced stromal cells bound threefold higher numbers of CFU-GM and BFU-E, this increase being abrogated by anti-alpha 4 beta 1 and anti-VCAM-1 antibodies. In addition, the adhesion to stroma of more immature progenitors, the long-term culture initiating cells, also occurred through an alpha 4 beta 1/VCAM-1-dependent mechanism. These studies identify an adhesion mechanism of potential importance in the localization of primitive progenitors within the hematopoietic microenvironment.

scholarly journals Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 388-395 ◽  
Author(s):  
PJ Simmons ◽  
B Masinovsky ◽  
BM Longenecker ◽  
R Berenson ◽  
B Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Stromal Cells ◽  
Cell Adhesion Molecule ◽  
Marrow Stromal Cells ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Cd34 Cells ◽  
Cell Adhesion Molecule 1

Abstract Human bone marrow-derived CD34+ cells were analyzed for the expression of the beta 1-family of integrin adhesion molecules. Integrin alpha 4 beta 1 was consistently expressed by greater than 90% of CD34+ cells, including essentially all assayable granulocyte-macrophage colony- forming cells (CFU-GM) and erythroid bursts (BFU-E) as shown by fluorescence-activated cell sorting studies. Adhesion of highly enriched CD34+ cells to cultured allogeneic marrow stromal cells was largely inhibited both by monoclonal antibody to alpha 4 beta 1 and to vascular cell adhesion molecule-1 (VCAM-1), a ligand for alpha 4 beta 1. VCAM-1 was found to be expressed by bone marrow stromal elements in vitro both constitutively at low level and at high levels after treatment with cytokines. Induction of VCAM-1 was cytokine- and time- dependent with maximum levels being obtained after 4 hours of exposure to a combination of interleukin-4 and tumor necrosis factor-alpha. Cytokine-induced stromal cells bound threefold higher numbers of CFU-GM and BFU-E, this increase being abrogated by anti-alpha 4 beta 1 and anti-VCAM-1 antibodies. In addition, the adhesion to stroma of more immature progenitors, the long-term culture initiating cells, also occurred through an alpha 4 beta 1/VCAM-1-dependent mechanism. These studies identify an adhesion mechanism of potential importance in the localization of primitive progenitors within the hematopoietic microenvironment.

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