Tactile Stimulation on Adulthood Modifies the HPA Axis, Neurotrophic Factors, and GFAP Signaling Reverting Depression-Like Behavior in Female Rats

2019 ◽  
Vol 56 (9) ◽  
pp. 6239-6250 ◽  
Author(s):  
Kr. Roversi ◽  
Caren Tatiane de David Antoniazzi ◽  
L. H. Milanesi ◽  
H. Z. Rosa ◽  
M. Kronbauer ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Neurotrophic Factors ◽  
Tactile Stimulation ◽  
Female Rats

LPS reduces BDNF and VEGF expression in the structures of the HPA axis of chronic social stressed female rats

Neuropeptides ◽  
2015 ◽  
Vol 54 ◽  
pp. 17-27 ◽  
Author(s):  
Marta M. Nowacka ◽  
Monika Paul-Samojedny ◽  
Anna M. Bielecka ◽  
Danuta Plewka ◽  
Piotr Czekaj ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Female Rats ◽  
Vegf Expression ◽  
Stressed Female

scholarly journals Overexpression of Corticotropin Releasing Factor in the Central Nucleus of the Amygdala Advances Puberty and Disrupts Reproductive Cycles in Female Rats

Endocrinology ◽  
2014 ◽  
Vol 155 (10) ◽  
pp. 3934-3944 ◽  
Author(s):  
X. F. Li ◽  
M. H. Hu ◽  
S. Y. Li ◽  
C. Geach ◽  
A. Hikima ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Hpa Axis ◽  
Pulse Generator ◽  
Female Rats ◽  
Central Nucleus ◽  
Ovariectomized Rats ◽  
Human Society ◽  
Central Administration ◽  
Corticosterone Secretion ◽  
Stress Changes

Abstract Prolonged exposure to environmental stress activates the hypothalamic-pituitary-adrenal (HPA) axis and generally disrupts the hypothalamic-pituitary-gonadal axis. Because CRF expression in the central nucleus of the amygdala (CeA) is a key modulator in adaptation to chronic stress, and central administration of CRF inhibits the hypothalamic GnRH pulse generator, we tested the hypothesis that overexpression of CRF in the CeA of female rats alters anxiety behavior, dysregulates the HPA axis response to stress, changes pubertal timing, and disrupts reproduction. We used a lentiviral vector to increase CRF expression site specifically in the CeA of preweaning (postnatal day 12) female rats. Overexpression of CRF in the CeA increased anxiety-like behavior in peripubertal rats shown by a reduction in time spent in the open arms of the elevated plus maze and a decrease in social interaction. Paradoxically, puberty onset was advanced but followed by irregular estrous cyclicity and an absence of spontaneous preovulatory LH surges associated with proestrous vaginal cytology in rats overexpressing CRF. Despite the absence of change in basal corticosterone secretion or induced by stress (lipopolysaccharide or restraint), overexpression of CRF in the CeA significantly decreased lipopolysaccharide, but not restraint, stress-induced suppression of pulsatile LH secretion in postpubertal ovariectomized rats, indicating a differential stress responsivity of the GnRH pulse generator to immunological stress and a potential adaptation of the HPA axis to chronic activation of amygdaloid CRF. These data suggest that the expression profile of this key limbic brain CRF system might contribute to the complex neural mechanisms underlying the increasing incidence of early onset of puberty on the one hand and infertility on the other attributed to chronic stress in modern human society.

Neurotrophic factors in the posterodorsal medial amygdala of male and cycling female rats

2020 ◽  
Vol 155 ◽  
pp. 92-101
Author(s):  
Mariana Zancan ◽  
Dinara J. Moura ◽  
Ana Moira Morás ◽  
Luiza Steffens ◽  
Ana Carolina de Moura ◽  
...  
Keyword(s):  
Neurotrophic Factors ◽  
Female Rats ◽  
Medial Amygdala

scholarly journals N-3 Polyunsaturated Fatty Acid Improved Depression via Neuroendocrine and Serotonergic System in Post-Menopausal Rats with Maternal Separation and Chronic Mild Stress

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1199-1199
Author(s):  
Jeong-Eun Choi ◽  
Yongsoon Park
Keyword(s):  
Hpa Axis ◽  
Maternal Separation ◽  
Chronic Mild Stress ◽  
Female Rats ◽  
Serotonin Level ◽  
Mild Stress ◽  
Total N ◽  
Serotonergic Pathway ◽  
Post Menopausal ◽  
Hpa Axis Activity

Abstract Objectives The purpose of the present study was to investigate the hypothesis that lifetime n-3 polyunsaturated fatty acids (PUFA) intake improved depression through serotonergic pathway in post-menopausal rats with chronic mild stress (CMS) and maternal separation (MS). Methods Female rats were fed diets with 0% or 1 energy % n-3 PUFA during lifetime from embryonic day (ED) 0 to postnatal day (PND) 112, or 1% n-3 PUFA before weaning (ED 0-PND 20), or after weaning (PND 20–112). The rats in four diet group were allocated to brief separation from dam (non-MS group) or long-term separation (MS group) on PND 2–14, and then underwent CMS on PND 91–105 after ovariectomy. Thus, there were eight groups in total (n = 8/group). Results MS + CMS increased depressive behaviors, and modified hypothalamic-pituitary-adrenal (HPA) axis activity, inflammation, serotonergic and glutamatergic neurotransmission, and related miRNAs as compared to CMS alone. N-3 PUFA decreased depressive behaviors by decreasing immobility while increasing swimming during forced swim test, and increasing sucrose preference in rats with MS + CMS and with CMS. N-3 PUFA decreased HPA axis activity by modifying expressions of corticotrophin releasing factor and glucocorticoid receptor, and levels of adrenocorticotropic hormone and corticosterone. N-3 PUFA also reduced levels of TNF-α, IL-1β, IL-6, PGE2, and miRNA-218, and increased serotonergic neurotransmission, including expressions of cAMP response element binding protein, brain-derived neurotrophic factor and serotonin 1A receptor, and serotonin level, and expression of miRNA-155. In addition, lifetime supplementation of n-3 PUFA had greater effect than pre- or post-supplementation. N-3 PUFA had no effect on glutamatergic pathway including α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor and N-methyl-D-aspartate receptor. Conclusions The present study suggested that lifetime n-3 PUFA improved depression in post-menopausal rats with MS + CMS through modulation of serotonergic pathway by decreasing HPA axis activity but not glutamatergic pathway. Funding Sources This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1A2B6002486).

Galantamine Effects on Memory, Spatial Cue Utilization, and Neurotrophic Factors in Aged Female Rats

2007 ◽  
Vol 16 (3) ◽  
pp. 197-205 ◽  
Author(s):  
K. L. French ◽  
H. A. Bimonte-Nelson ◽  
A.-Ch. Granholm
Keyword(s):  
Neurotrophic Factors ◽  
Female Rats ◽  
Cue Utilization

scholarly journals Effect of oestrogen-dependent vasopressin on HPA axis in the median eminence of female rats

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kazuaki Nishimura ◽  
Kiyoshi Yoshino ◽  
Kenya Sanada ◽  
Hiroki Beppu ◽  
Yasuki Akiyama ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Median Eminence ◽  
Female Rats

scholarly journals Rats Lacking Dopamine Transporter Display Increased Vulnerability and Aberrant Autonomic Response to Acute Stress

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 842
Author(s):  
Placido Illiano ◽  
Gregory E. Bigford ◽  
Raul R. Gainetdinov ◽  
Marta Pardo
Keyword(s):  
Dopamine Transporter ◽  
Hpa Axis ◽  
Acute Stress ◽  
Female Rats ◽  
Autonomic Response ◽  
Before And After ◽  
Response To Stress ◽  
Vulnerability To Stress ◽  
Body Temperature Increase ◽  
Reduced Temperature

The activity of the hypothalamus–pituitary–adrenal (HPA) axis is pivotal in homeostasis and presides the adaptative response to stress. Dopamine Transporter (DAT) plays a key role in the regulation of the HPA axis. We used young adult female DAT Knockout (KO) rats to assess the effects of DAT ablation (partial, heterozygous DAT+/-, or total, homozygous DAT-/-) on vulnerability to stress. DAT-/- rats show profound dysregulation of pituitary homeostasis, in the presence of elevated peripheral corticosterone, before and after acute restraint stress. During stress, DAT-/- rats show abnormal autonomic response at either respiratory and cardiovascular level, and delayed body temperature increase. DAT+/- rats display minor changes of hypophyseal homeostatic mechanisms. These rats display a similar pituitary activation to that of the control animals, albeit in the presence of higher release of peripheral corticosterone than DAT-/- after stress, and reduced temperature during stress. Our data indicate that DAT regulates the HPA axis at both the central and peripheral level, including autonomic function during stress. In particular, the partial deletion of DAT results in increased vulnerability to stress in female rats, which display central and peripheral alterations that are reminiscent of PTSD, and they might provide new insights in the pathophysiology of this disorder.

scholarly journals Regulation of the Hypothalamic-Pituitary-Adrenal Axis Circadian Rhythm by Endocannabinoids Is Sexually Diergic

Endocrinology ◽  
2010 ◽  
Vol 151 (8) ◽  
pp. 3720-3727 ◽  
Author(s):  
Helen C. Atkinson ◽  
James D. Leggett ◽  
Susan A. Wood ◽  
Emma S. Castrique ◽  
Yvonne M. Kershaw ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Cannabinoid Receptor ◽  
Time Of Day ◽  
Female Rats ◽  
Male Rats ◽  
Acute Administration ◽  
Sampling System ◽  
Hypothalamic Pituitary Adrenal ◽  
Male And Female Rats ◽  
Cb1 Antagonist

We have examined the effects of acute administration of the cannabinoid receptor type 1 (CB1) antagonist AM251 on the rat hypothalamic-pituitary-adrenal (HPA) axis with respect to both gender and time of day. Blood samples were collected from conscious male and female rats every 5 min using an automated blood sampling system, and corticosterone concentrations were determined. In male rats, there was a distinct diurnal effect of AM251 with a greater activation of the HPA axis in the morning (diurnal trough) compared with the evening (diurnal peak). At both times of the day, circulating corticosterone concentrations were elevated for approximately 4 h after AM251 administration. In female rats, there was also diurnal variation in the activation of the HPA axis; however, these effects were not as profound as those in males. Corticosterone concentrations were only slightly elevated at the diurnal trough and for a shorter time period than in males (2 compared with 4 h). Moreover, there was no effect of AM251 on corticosterone concentrations when administered at the diurnal peak. Subsequent studies, only in males, in which both ACTH and corticosterone were measured, confirmed that the effects of AM251 on corticosterone were mediated by ACTH. Moreover, the elevation of both ACTH and corticosterone could be replicated using another CB1 antagonist, AM281. These data demonstrate that the extent and duration of HPA axis activation after CB1 blockade are clearly dependent on both gender and time of day.

Sign in / Sign up

Export Citation Format

Share Document