The HepaRG cell line: a valuable in vitro tool for hepatitis virus infection studies

2013 ◽  
Vol 7 (2) ◽  
pp. 394-399
Author(s):  
Liesbeth Ceelen ◽  
Marusya Lieveld ◽  
Ramses Forsyth ◽  
Mathieu Vinken
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
Hepatitis Virus ◽  
Heparg Cell ◽  
Heparg Cell Line ◽  
Hepatitis Virus Infection

scholarly journals In-vitro evaluation of DDI with cobicistat and ritonavir using heparg cell line

2015 ◽  
Vol 37 (8) ◽  
pp. e98
Author(s):  
F. Storelli ◽  
C. Bruggmann ◽  
F. Doffey-Lazeyras ◽  
C. Samer ◽  
J. Desmeules ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vitro Evaluation ◽  
Heparg Cell ◽  
Heparg Cell Line

The HepaRG cell line, a superior in vitro model to L-02, HepG2 and hiHeps cell lines for assessing drug-induced liver injury

2016 ◽  
Vol 32 (1) ◽  
pp. 37-59 ◽  
Author(s):  
Yu Wu ◽  
Xing-chao Geng ◽  
Ju-feng Wang ◽  
Yu-fa Miao ◽  
Yan-li Lu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Cell Line ◽  
Cell Lines ◽  
In Vitro Model ◽  
Drug Induced ◽  
Heparg Cell ◽  
Drug Induced Liver Injury ◽  
Heparg Cell Line ◽  
Vitro Model

HepaRG cell line as an in vitro model for screening drug–drug interactions mediated by metabolic induction: Amiodarone used as a model substance

2014 ◽  
Vol 28 (8) ◽  
pp. 1531-1535 ◽  
Author(s):  
Ana Ferreira ◽  
Márcio Rodrigues ◽  
Samuel Silvestre ◽  
Amílcar Falcão ◽  
Gilberto Alves
Keyword(s):  
Drug Interactions ◽  
Cell Line ◽  
In Vitro Model ◽  
Heparg Cell ◽  
Heparg Cell Line ◽  
Model Substance ◽  
Vitro Model ◽  
Metabolic Induction

Mouse Hepatitis Virus Infection Suppresses Modulation of Mouse Spleen T- Cell Activation

1988 ◽  
Author(s):  
Joan M. Cook-Mills ◽  
Hidayatulla G. Munshi ◽  
Robert L. Perlman ◽  
Donald A. Chambers
Keyword(s):  
T Cell ◽  
Virus Infection ◽  
T Cell Activation ◽  
Cell Activation ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Mouse Spleen ◽  
Hepatitis Virus Infection

scholarly journals Antiviral Activities of Oral 1-O-Hexadecylpropanediol-3-Phosphoacyclovir and Acyclovir in Woodchucks with Chronic Woodchuck Hepatitis Virus Infection

2000 ◽  
Vol 44 (7) ◽  
pp. 1964-1969 ◽  
Author(s):  
Karl Y. Hostetler ◽  
James R. Beadle ◽  
William E. Hornbuckle ◽  
Christine A. Bellezza ◽  
Ilia A. Tochkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hepatitis Virus ◽  
Response To Therapy ◽  
Hydroxyl Group ◽  
Woodchuck Hepatitis Virus ◽  
Acyclovir Treatment ◽  
B Virus ◽  
Hepatitis Virus Infection

ABSTRACT Acyclovir triphosphate is a potent inhibitor of hepatitis B virus DNA polymerase, but acyclovir treatment provides no benefit in patients with hepatitis B virus infection. This is due in part to the fact that hepatitis B virus, unlike herpes simplex virus, does not code for a viral thymidine kinase which catalyzes the initial phosphorylation of acyclovir. We synthesized 1-O-octadecyl-sn-glycero-3-phospho (3-P)-acyclovir and found that it was highly active in reducing hepatitis B virus replication in 2.2.15 cells, while acyclovir was inactive. The greater antiviral activity of 1-O-octadecyl-sn-glycero-3-P-acyclovir appeared to be due to liver cell metabolism of the compound to acyclovir monophosphate (K. Y. Hostetler et al., Biochem. Pharmacol. 53:1815–1822, 1997). However, a closely related compound without a hydroxyl group at the sn-2 position of glycerol, 1-O-hexadecylpropanediol-3-P-acyclovir, was more active and selective in 2.2.15 cells in vitro. In this study, we treated woodchucks chronically infected with woodchuck hepatitis virus with increasing oral doses of 1-O-hexadecylpropanediol-3-P-acyclovir and assessed the response to therapy versus acyclovir or a placebo. At a dosage of 10 mg/kg of body weight twice a day, the test compound significantly inhibited viral replication in vivo, as indicated by a 95% reduction in serum woodchuck hepatitis virus DNA levels and by a 54% reduction in levels of woodchuck hepatitis virus replicative intermediates in the liver. Higher doses were somewhat less effective. In contrast, 20 mg of acyclovir/kg twice daily, a 5.3-fold-higher molar dosage, had no demonstrable activity against woodchuck hepatitis virus. Oral 1-O-hexadecylpropanediol-3-P-acyclovir appeared to be safe and effective in chronic woodchuck hepatitis virus infection.

scholarly journals Effect of persistent mouse hepatitis virus infection on MHC Class I expression in murine astrocytes

1995 ◽  
Vol 40 (1) ◽  
pp. 10-21 ◽  
Author(s):  
J. Correale ◽  
S. Li ◽  
L. P. Weiner ◽  
Wendy Gilmore
Keyword(s):  
Virus Infection ◽  
Mhc Class I ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Class I ◽  
Hepatitis Virus Infection
Sign in / Sign up

Export Citation Format

Share Document