15N, 13C, and 1H resonance assignments of Jarastatin: a disintegrin of Bothrops jararaca

2021 ◽  
Author(s):  
Ariana Azevedo Vasconcelos ◽  
Barbara Barbosa Succar ◽  
Leonardo Bartkevihi di Piero ◽  
Eleonora Kurtenbach ◽  
Russolina Benedeta Zingali ◽  
...  
Keyword(s):  
Resonance Assignments ◽  
Bothrops Jararaca

Properties of Fibrinogen Cleaved by Jararhagin, a Metalloproteinase from the Venom of Bothrops jararaca

1994 ◽  
Vol 72 (02) ◽  
pp. 244-249 ◽  
Author(s):  
Aura S Kamiguti ◽  
Joseph R Slupsky ◽  
Mirko Zuzel ◽  
Charles R M Hay
Keyword(s):  
Platelet Aggregation ◽  
Platelet Function ◽  
Fibrin Clot ◽  
Clot Formation ◽  
Human Fibrinogen ◽  
Activated Platelets ◽  
Bothrops Jararaca ◽  
Platelet Binding ◽  
Fibrin Monomers ◽  
Fibrinogen Molecule

SummaryHaemorrhagic metalloproteinases from Bothrops jararaca and other venoms degrade vessel-wall and plasma proteins involved in platelet plug and fibrin clot formation. These enzymes also cause proteolytic digestion of fibrinogen which has been suggested to cause defective platelet function. Fibrinogen degradation by jararhagin, a metalloproteinase from B. jararaca, and the effect of jararhagin fibrinogenolysis on both platelet aggregation and fibrin clot formation were investigated. Jararhagin was found to cleave human fibrinogen in the C-terminal region of the Aα-chain giving rise to a 285-290 kDa fibrinogen molecule lacking the Aα-chain RGD 572-574 platelet-binding site. Platelet binding and aggregation of ADP-activated platelets is unaffected by this modification. This indicates that the lost site is not essential for platelet aggregation, and that the remaining platelet binding sites located in the N-terminal portion of Aα chains (RGD 95-97) and the C-terminal of γ chains (dodecapeptide 400-411) are unaffected by jararhagin-digestion of fibrinogen. Fibrin clot formation with thrombin of this remnant fibrinogen molecule was defective, with poor polymerization of fibrin monomers but normal release of FPA. The abnormal polymerization could be explained by the loss of one of the two complementary polymerization sites required for side-by-side association of fibrin protofibrils. Jararhagin-induced inhibition of platelet function, an important cause of haemorrhage in envenomed patients, is not caused by proteolysis of fibrinogen, as had been thought, and the mechanism remains to be elucidated.

1H, 15N, 13C resonance assignments for pyrazinoic acid binding domain of ribosomal protein S1 from Mycobacterium tuberculosis

2016 ◽  
Vol 10 (2) ◽  
pp. 321-324 ◽  
Author(s):  
Biling Huang ◽  
Jinglin Fu ◽  
Chenyun Guo ◽  
Xueji Wu ◽  
Donghai Lin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Ribosomal Protein ◽  
Resonance Assignments ◽  
Binding Domain ◽  
Ribosomal Protein S1 ◽  
Pyrazinoic Acid

scholarly journals The 1H, 15N, and 13C resonance assignments of the N-terminal domain of the nucleocapsid protein from the Middle East respiratory syndrome coronavirus

2021 ◽  
Author(s):  
Talita Stelling de Araujo ◽  
Glauce Moreno Barbosa ◽  
Karoline Sanches ◽  
Jéssica M. Azevedo ◽  
Katia Maria dos Santos Cabral ◽  
...  
Keyword(s):  
Middle East ◽  
Nucleocapsid Protein ◽  
Resonance Assignments ◽  
Middle East Respiratory Syndrome ◽  
Terminal Domain

Aliphatic 1H and 13C resonance assignments for the 26-10 antibody VL domain derived from heteronuclear multidimensional NMR spectroscopy

1993 ◽  
Vol 3 (1) ◽  
Author(s):  
KeithL. Constantine ◽  
Valentina Goldfarb ◽  
Michael Wittekind ◽  
MarkS. Friedrichs ◽  
James Anthony ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Resonance Assignments ◽  
Multidimensional Nmr ◽  
Multidimensional Nmr Spectroscopy ◽  
Heteronuclear Multidimensional Nmr

1H, 15N and 13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca type 2 secretion system

2017 ◽  
Vol 11 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Aracelys López-Castilla ◽  
Bruno Vitorge ◽  
Léa Khoury ◽  
Nelly Morellet ◽  
Olivera Francetic ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Klebsiella Oxytoca ◽  
Resonance Assignments ◽  
Secretion System ◽  
Type 2 Secretion
Sign in / Sign up

Export Citation Format

Share Document