Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Olive and Refined Pomace Olive Oils with Modified Low Temperature and Ultrasound-Assisted Liquid–Liquid Extraction Method Followed by the HPLC/FLD

2015 ◽  
Vol 9 (5) ◽  
pp. 1220-1227 ◽  
Author(s):  
Zohreh Taghvaee ◽  
Zahra Piravivanak ◽  
Karamatollah Rezaei ◽  
Mohammad Faraji
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Low Temperature ◽  
Aromatic Hydrocarbons ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Olive Oils ◽  
Liquid Liquid Extraction ◽  
Polycyclic Aromatic ◽  
Ultrasound Assisted

scholarly journals Sample Preparation Improvement in Polycyclic Aromatic Hydrocarbons Determination in Olive Oils by Gel Permeation Chromatography and Liquid Chromatography with Fluorescence Detection

2005 ◽  
Vol 88 (4) ◽  
pp. 1247-1254 ◽  
Author(s):  
Sandra Martinez-López ◽  
Asuncion Morales-Noé ◽  
Agustin Pastor-Garcia ◽  
Angel Morales-Rubio ◽  
Miguel de la Guardia
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Limit Of Detection ◽  
Gel Permeation Chromatography ◽  
Liquid Extraction ◽  
Fluorometric Detection ◽  
Olive Oils ◽  
Liquid Liquid Extraction ◽  
Gel Permeation ◽  
Polycyclic Aromatic

Abstract The determination of 15 polycyclic aromatic hydrocarbons (PAHs) in olive oil samples has been improved in order to obtain a fast methodology with a low limit of detection through the combination of liquid–liquid extraction with acetonitrile and preparative gel permeation chromatography (GPC) prior to the injection of purified extracts into a C18 column. Acetonitrile–water was used as the mobile phase with a gradient from 50 to 95%, w/w, acetonitrile in 30 min. The oven temperature was maintained at 15°C, and fluorometric detection was made at a fixed excitation wavelength of 264 nm and variable, optimal emission wavelength for each analyte ranging from 352 nm for 11-H-benzo(b)fluorene to 500 nm for indeno(1,2,3-cd)pyrene. Recovery for all the compounds studied varied from 75 to 111%, and limit of detection values from 0.05 ng/g for benzo(k)fluoranthene to 0.48 ng/g for indeno(1,2,3-cd)pyrene, corresponding to 0.09 ng/g benzo(a)pyrene. Results were compared with those obtained by liquid–liquid extraction followed by a cleanup on silica and a direct GPC treatment of oil samples diluted in dichloromethane, 2 other methodologies that are appropriate for quantifying PAHs in olive oils. However, the proposed method improves the determination limits, reduces the time of analysis, and provides a highly stable baseline for sample chromatograms.

scholarly journals Qualitative TLC determination of some polycyclic aromatic hydrocarbons in sugar-beet

2005 ◽  
Vol 70 (10) ◽  
pp. 1237-1242 ◽  
Author(s):  
Eva Loncar ◽  
Ljiljana Kolarov ◽  
Radomir Malbasa ◽  
Biljana Skrbic
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Sugar Beet ◽  
Silica Gel ◽  
Aromatic Hydrocarbons ◽  
Liquid Extraction ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Liquid Liquid Extraction ◽  
Polycyclic Aromatic ◽  
Layer Chromatography

The presence of polycyclic or polynuclear aromatic hydrocarbons (PAHs) were investigated in sugar-beet from a local sugar factory in the district of Vojvodina. The sugar-beet was cultivated on areas near roads with intensive traffic. The procedure for the preparation and determination of these compounds included saponification of the sample, several liquid?liquid extraction systems and a silica gel column clean-up. The purified sample solution was analysed by thin layer chromatography (TLC) on silica gel with cyclohexane as the developing solvent. Benzo(b)fluoranthene and benzo(a)anthracene and/or benzo(a)pyrene were detected at concentrations greater than the allowed limits in food.

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