Expression of green fluorescent protein under the regulation of human locus control region elements HS2 and HS3 in transgenic mice

2008 ◽  
Vol 88 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Jingbin Yan ◽  
Yanping Xiao ◽  
Shu Wang ◽  
Zhijuan Gong ◽  
Shuzheng Huang ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Control Region ◽  
Fluorescent Protein ◽  
Locus Control Region ◽  
Green Fluorescent

High level Purkinje cell specific expression of green fluorescent protein in transgenic mice

2001 ◽  
Vol 115 (6) ◽  
pp. 455-464 ◽  
Author(s):  
Xulun Zhang ◽  
Stephan L. Baader ◽  
Feng Bian ◽  
Wolfgang Müller ◽  
John Oberdick
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Purkinje Cell ◽  
Fluorescent Protein ◽  
Specific Expression ◽  
Green Fluorescent ◽  
High Level

BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons

2006 ◽  
Vol 27 (3) ◽  
pp. 391-397 ◽  
Author(s):  
Yvonne N. Tallini ◽  
Bo Shui ◽  
Kai Su Greene ◽  
Ke-Yu Deng ◽  
Robert Doran ◽  
...  
Keyword(s):  
Nervous System ◽  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Peripheral Nervous System ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Cholinergic Neurons ◽  
Autonomic Nerves ◽  
Organ Systems ◽  
Green Fluorescent

The peripheral nervous system has complex and intricate ramifications throughout many target organ systems. To date this system has not been effectively labeled by genetic markers, due largely to inadequate transcriptional specification by minimum promoter constructs. Here we describe transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements, by knock-in of eGFP within a bacterial artificial chromosome (BAC) spanning the ChAT locus and expression of this construct as a transgene. eGFP is expressed in ChATBAC-eGFP mice in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems. Individual epithelial cells and a subset of lymphocytes within the gastrointestinal and airway mucosa are also labeled, indicating genetic evidence of acetylcholine biosynthesis. Central and peripheral neurons were observed as early as 10.5 days postcoitus in the developing mouse embryo. ChATBAC-eGFP mice allow excellent visualization of all cholinergic elements of the peripheral nervous system, including the submucosal enteric plexus, preganglionic autonomic nerves, and skeletal, cardiac, and smooth muscle neuromuscular junctions. These mice should be useful for in vivo studies of cholinergic neurotransmission and neuromuscular coupling. Moreover, this genetic strategy allows the selective expression and conditional inactivation of genes of interest in cholinergic nerves of the central nervous system and peripheral nervous system.

Green fluorescent protein-transgenic mice: immune functions and their application to studies of lymphocyte development

2000 ◽  
Vol 70 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Naoto Kawakami ◽  
Naoki Sakane ◽  
Fumiko Nishizawa ◽  
Mutsumi Iwao ◽  
So-ichiro Fukada ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Lymphocyte Development ◽  
Immune Functions ◽  
Green Fluorescent

scholarly journals Novel Hippocampal Interneuronal Subtypes Identified Using Transgenic Mice That Express Green Fluorescent Protein in GABAergic Interneurons

2000 ◽  
Vol 20 (9) ◽  
pp. 3354-3368 ◽  
Author(s):  
Anthony A. Oliva ◽  
Minghui Jiang ◽  
Trang Lam ◽  
Karen L. Smith ◽  
John W. Swann
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Gabaergic Interneurons ◽  
Green Fluorescent

Visualization of axonal guidance and patterning in transgenic mice expressing green fluorescent protein

1998 ◽  
Vol 31 ◽  
pp. S297
Author(s):  
Yuki Hattori ◽  
Michiko Saito ◽  
Natsuki Matsushita ◽  
Kazuto Kobayashi
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Axonal Guidance ◽  
Green Fluorescent

scholarly journals Green fluorescent protein as a marker in transgenic mice

1995 ◽  
Vol 37 (4) ◽  
pp. 455-459 ◽  
Author(s):  
Masahito Ikawa ◽  
Katsuya Kominami ◽  
Yasuhide Yoshimura ◽  
Keiichi Tanaka ◽  
Yoshitake Nishimune ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Green Fluorescent
Sign in / Sign up

Export Citation Format

Share Document