Characterization of Multi-Sourced Diclofenac Sodium Extended-Release Tablet Dissolution Profiles: A New Approach to Establish an In vitro-In vivo Correlation Based on Multiple Integral Response Surface

2015 ◽  
Vol 10 (4) ◽  
pp. 302-312 ◽  
Author(s):  
Baoming Ning ◽  
Xi Liu ◽  
Hansen Luan ◽  
Jiasheng Tu ◽  
Huiyi Li ◽  
...  
Keyword(s):  
Extended Release ◽  
Diclofenac Sodium ◽  
Multiple Integral ◽  
New Approach ◽  
Tablet Dissolution ◽  
Integral Response ◽  
Extended Release Tablet

scholarly journals In vivo measurement of intrauterine pressure by telemetry: a new approach for studying parturition in mouse models

2010 ◽  
Vol 42 (2) ◽  
pp. 310-316 ◽  
Author(s):  
Stephanie L. Pierce ◽  
William Kutschke ◽  
Rafael Cabeza ◽  
Sarah K. England
Keyword(s):  
Mouse Models ◽  
Accurate Method ◽  
Isometric Tension ◽  
Mouse Uterus ◽  
New Approach ◽  
In Vivo Measurement ◽  
Intrauterine Pressure

Transgenic and knockout mouse models have proven useful in the study of genes necessary for parturition—including genes that affect the timing and/or progression of labor contractions. However, taking full advantage of these models will require a detailed characterization of the contractile patterns in the mouse uterus. Currently the best methodology for this has been measurement of isometric tension in isolated muscle strips in vitro. However, this methodology does not provide a real-time measure of changes in uterine pressure over the course of pregnancy. Recent advances have opened the possibility of using radiotelemetric devices to more accurately and comprehensively study intrauterine pressure in vivo. We tested the effectiveness of this technology in the mouse, in both wild-type (WT) mice and a mouse model of defective parturition (SK3 channel-overexpressing mice), after surgical implant of telemetry transmitters into the uterine horn. Continuous recordings from day 18 of pregnancy through delivery revealed that WT mice typically deliver during the 12-h dark cycle after 19.5 days postcoitum. In these mice, intrauterine pressure gradually increases during this cycle, to threefold greater than that measured during the 12-h cycle before delivery. SK3-overexpressing mice, by contrast, exhibited lower intrauterine pressure over the same period. These results are consistent with the outcome of previous in vitro studies, and they indicate that telemetry is an accurate method for measuring uterine contraction, and hence parturition, in mice. The use of this technology will lead to important novel insights into changes in intrauterine pressure during the course of pregnancy.

Development of a Level A in Vitro-in Vivo Correlation for Veliparib (ABT-888) Extended Release Tablet Formulation

2017 ◽  
Vol 34 (6) ◽  
pp. 1187-1192 ◽  
Author(s):  
Rajendar K. Mittapalli ◽  
Silpa Nuthalapati ◽  
Alyssa E. Delke DeBord ◽  
Hao Xiong
Keyword(s):  
Extended Release ◽  
Tablet Formulation ◽  
Extended Release Tablet ◽  
Release Tablet

Development of In Vitro-In Vivo Correlation for Potassium Chloride Extended Release Tablet Formulation Using Urinary Pharmacokinetic Data

2017 ◽  
Vol 34 (7) ◽  
pp. 1527-1533 ◽  
Author(s):  
Rajendar K. Mittapalli ◽  
Patrick Marroum ◽  
Yihong Qiu ◽  
Kathleen Apfelbaum ◽  
Hao Xiong
Keyword(s):  
Potassium Chloride ◽  
Extended Release ◽  
Tablet Formulation ◽  
Pharmacokinetic Data ◽  
Extended Release Tablet ◽  
Release Tablet

Once-daily propranolol extended-release tablet dosage form: formulation design and in vitro/in vivo investigation

2004 ◽  
Vol 58 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Yaw-Bin Huang ◽  
Yi-Hung Tsai ◽  
Wan-Chiech Yang ◽  
Jui-Sheng Chang ◽  
Pao-Chu Wu ◽  
...  
Keyword(s):  
Dosage Form ◽  
Extended Release ◽  
Formulation Design ◽  
Once Daily ◽  
Tablet Dosage ◽  
Extended Release Tablet ◽  
Release Tablet

Dosage form design and in vitro/in vivo evaluation of cevimeline extended-release tablet formulations

2010 ◽  
Vol 383 (1-2) ◽  
pp. 99-105 ◽  
Author(s):  
Shinichiro Tajiri ◽  
Taro Kanamaru ◽  
Kamada Makoto ◽  
Tsutomu Konno ◽  
Hiroaki Nakagami
Keyword(s):  
Dosage Form ◽  
Extended Release ◽  
In Vivo Evaluation ◽  
Dosage Form Design ◽  
Form Design ◽  
Tablet Formulations ◽  
Extended Release Tablet ◽  
Release Tablet

scholarly journals Prototyping of Bacillus megaterium genetic elements through automated cell-free characterization and Bayesian modelling

2016 ◽  
Author(s):  
Simon J. Moore ◽  
James T. MacDonald ◽  
Sarah Weinecke ◽  
Nicolas Kylilis ◽  
Karen M. Polizzi ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Statistical Approach ◽  
Model Parameters ◽  
Experimental Conditions ◽  
New Approach ◽  
Liquid Handling ◽  
Translation Systems

AbstractAutomation and factorial experimental design together with cell-free in vitro transcription-translation systems offers a new route to the precise characterization of regulatory components. This now presents a new opportunity to illuminate the genetic circuitry from arcane microbial chassis, which are difficult to assess in vivo. One such host, Bacillus megaterium, is a giant microbe with industrial potential as a producer of recombinant proteins at gram per litre scale. Herein, we establish a B. megaterium cell-free platform and characterize a refactored xylose-repressor circuit using acoustic liquid handling robotics to simultaneously monitor 324 reactions in vitro. To accurately describe the system, we have applied a Bayesian statistical approach to infer model parameters by simultaneously using information from multiple experimental conditions. These developments now open up a new approach for the rapid and accurate characterization of genetic circuitry using cell-free reactions from unusual microbial cell chasses for bespoke applications.

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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