Expression and purification of human papillomavirus 18 L1 virus-like particle from Saccharomyces cerevisiae

2008 ◽  
Vol 31 (2) ◽  
pp. 205-209 ◽  
Author(s):  
Mi-Kyung Woo ◽  
Jung-Mo An ◽  
Jun-Dong Kim ◽  
Sue-Nie Park ◽  
Hong-Jin Kim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Human Papillomavirus ◽  
Expression And Purification ◽  
Virus Like Particle ◽  
Human Papillomavirus 18

scholarly journals A Chimeric 18L1-45RG1 Virus-Like Particle Vaccine Cross-Protects against Oncogenic Alpha-7 Human Papillomavirus Types

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120152 ◽  
Author(s):  
Bettina Huber ◽  
Christina Schellenbacher ◽  
Christoph Jindra ◽  
Dieter Fink ◽  
Saeed Shafti-Keramat ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Virus Like Particle ◽  
Alpha 7

scholarly journals Head-to-Head Comparison of Modular Vaccines Developed Using Different Capsid Virus-Like Particle Backbones and Antigen Conjugation Systems

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 539
Author(s):  
Laurits Fredsgaard ◽  
Louise Goksøyr ◽  
Susan Thrane ◽  
Kara-Lee Aves ◽  
Thor G. Theander ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Papillomavirus ◽  
Immune Responses ◽  
Capsid Protein ◽  
Major Capsid Protein ◽  
Molecular Scaffolds ◽  
Conjugation System ◽  
Virus Like Particles ◽  
Virus Like Particle ◽  
Specific Igg

Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains unknown to what extent the employed cVLP backbone and conjugation system may influence the immune response elicited against the displayed antigen. Here, we performed a head-to-head comparison of antigen-specific IgG responses elicited by modular cVLP-vaccines differing by their employed cVLP backbone or conjugation system, respectively. Covalent antigen conjugation (i.e., employing the SpyTag/SpyCatcher system) resulted in significantly higher antigen-specific IgG titers compared to when using affinity-based conjugation (i.e., using biotin/streptavidin). The cVLP backbone also influenced the antigen-specific IgG response. Specifically, vaccines based on the bacteriophage AP205 cVLP elicited significantly higher antigen-specific IgG compared to corresponding vaccines using the human papillomavirus major capsid protein (HPV L1) cVLP. In addition, the AP205 cVLP platform mediated induction of antigen-specific IgG with a different subclass profile (i.e., higher IgG2a and IgG2b) compared to HPV L1 cVLP. These results demonstrate that the cVLP backbone and conjugation system can individually affect the IgG response elicited against a displayed antigen. These data will aid the understanding and process of tailoring modular cVLP vaccines to achieve improved immune responses.

scholarly journals Vaccine synergy with virus-like particle and immune complex platforms for delivery of human papillomavirus L2 antigen

Vaccine ◽  
2019 ◽  
Vol 37 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Andrew G. Diamos ◽  
Dalia Larios ◽  
Lauren Brown ◽  
Jacquelyn Kilbourne ◽  
Hyun Soon Kim ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune Complex ◽  
Virus Like Particle

scholarly journals CD4+ T cells against human papillomavirus-18 E7 in patients with high-grade cervical lesions associate with the absence of the virus in the cervix

Immunology ◽  
2010 ◽  
pp. no-no ◽  
Author(s):  
Samantha Seresini ◽  
Massimo Origoni ◽  
Luigi Caputo ◽  
Flavia Lillo ◽  
Renato Longhi ◽  
...  
Keyword(s):  
T Cells ◽  
Human Papillomavirus ◽  
Cd4 T Cells ◽  
High Grade ◽  
Cervical Lesions ◽  
Human Papillomavirus 18

scholarly journals The Impact of Quadrivalent Human Papillomavirus (HPV; Types 6, 11, 16, and 18) L1 Virus‐Like Particle Vaccine on Infection and Disease Due to Oncogenic Nonvaccine HPV Types in Generally HPV‐Naive Women Aged 16–26 Years

2009 ◽  
Vol 199 (7) ◽  
pp. 926-935 ◽  
Author(s):  
Darron R. Brown ◽  
Susanne K. Kjaer ◽  
Kristján Sigurdsson ◽  
Ole‐Erik Iversen ◽  
Mauricio Hernandez‐Avila ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Virus Like Particle ◽  
Hpv Types ◽  
The Impact

scholarly journals Cloning, expression and purification of recombinant Slx4 from Saccharomyces cerevisiae (539.3)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Angel Diaz Martinez ◽  
Jose Renato Cussiol ◽  
Marcus Smolka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Expression And Purification

scholarly journals In SilicoDocking to Explicate Interface between Plant-Originated Inhibitors and E6 Oncogenic Protein of Highly Threatening Human Papillomavirus 18

2015 ◽  
Vol 13 (2) ◽  
pp. 60 ◽  
Author(s):  
Satish Kumar ◽  
Lingaraja Jena ◽  
Maheswata Sahoo ◽  
Mrunmayi Kakde ◽  
Sangeeta Daf ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Human Papillomavirus 18 ◽  
Oncogenic Protein
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