scholarly journals Role of ascorbic acid in human seminal plasma against the oxidative damage to the sperms

2009 ◽  
Vol 24 (3) ◽  
pp. 312-315 ◽  
Author(s):  
Piyali Das ◽  
A. R. Choudhari ◽  
A. Dhawan ◽  
Ramji Singh
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Seminal Plasma ◽  
Human Seminal Plasma

Investigations on the Functional Role of Angiotensin Converting Enzyme (ACE) in Human Seminal Plasma

1986 ◽  
pp. 477-485 ◽  
Author(s):  
Franz Krassnigg ◽  
Harald Niederhauser ◽  
Rainer Placzek ◽  
Julian Frick ◽  
Wolf-Bernhard Schill
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Seminal Plasma ◽  
Functional Role ◽  
Converting Enzyme ◽  
Human Seminal Plasma

The role of galectin-3 in spermatozoa-zona pellucida binding and its association with fertilization in vitro

2019 ◽  
Vol 25 (8) ◽  
pp. 458-470 ◽  
Author(s):  
Si Mei ◽  
Panyu Chen ◽  
Cheuk-Lun Lee ◽  
Weie Zhao ◽  
Ying Wang ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Zona Pellucida ◽  
Binding Capacity ◽  
Fertilization Rate ◽  
Clinical Samples ◽  
Fertilization In Vitro ◽  
Human Seminal Plasma ◽  
Sperm Surface ◽  
Galectin 3

AbstractHuman spermatozoa can fertilize an oocyte only after post-testicular maturation and capacitation. These processes involve dynamic modification and reorganization of the sperm plasma membrane, which allow them to bind to the zona pellucida (ZP) of the oocyte. Defective sperm-ZP binding is one of the major causes of male subfertility. Galectin-3 is a secretory lectin in human seminal plasma well known for its action on cell adhesion. The aim of this study was to determine the role of galectin-3 in spermatozoa-ZP interaction and its association with fertilization rate in clinical assisted reproduction. Our studies revealed that the acrosomal region of ejaculated and capacitated spermatozoa possess strong galectin-3 immunoreactivity, which is much stronger than that of epididymal spermatozoa. Expression of galectin-3 can also be detected on seminal plasma-derived extracellular vesicles (EVs) and can be transferred to the sperm surface. Blocking of sperm surface galectin-3 function by antibody or carbohydrate substrate reduced the ZP-binding capacity of spermatozoa. Purified galectin-3 is capable of binding to ZP, indicating that galectin-3 may serve as a cross-linking bridge between ZP glycans and sperm surface glycoproteins. Galectin-3 levels in seminal plasma-derived EVs were positively associated with fertilization rates. These results suggest that galectin-3 in EVs is transferred to the sperm surface during post-testicular maturation and plays a crucial role in spermatozoa-ZP binding after capacitation. Reduced galectin-3 expression in seminal plasma-derived EVs may be a cause behind a low fertilization rate. Further studies with more clinical samples are required to confirm the relationship between galectin-3 levels and IVF outcomes.

scholarly journals Water- and Fat-Soluble Antioxidants in Human Seminal Plasma and Serum of Fertile Males

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 96 ◽  
Author(s):  
Giacomo Lazzarino ◽  
Ilaria Listorti ◽  
Gabriele Bilotta ◽  
Talia Capozzolo ◽  
Angela Amorini ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Vitamin E ◽  
Vitamin C ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Venous Blood ◽  
Water Soluble ◽  
Human Seminal Plasma ◽  
Serum Ratio

Reactive oxygen species (ROS) are physiologically involved in functions like sperm maturation, capacitation and acrosome reaction, but their excess is involved in male infertility. Antioxidants in seminal plasma (SP) are an important factor balancing physiologic and harmful ROS activities. In this study, we determined and compared the full profiles of the water- and fat-soluble antioxidants in SP and serum of 15 healthy fertile subjects (ranging between the ages of 35 and 42 years). Ejaculates were obtained after 2–5 days of sexual abstinence. After liquefaction and withdrawal of an aliquot for the sperm count, samples were centrifuged to obtain SP. Thirty min after semen donation, a venous blood sample was collected from each subject. Donors with lower SP concentrations of ascorbic acid (n = 5) or α-tocopherol (n = 5) received a 4 week oral administration of either vitamin C (100 mg/day) or vitamin E (30 mg/day). They were then re-assayed to determine the SP and serum levels of ascorbic acid and α-tocopherol. SP and serum samples were properly processed and analyzed by HPLC methods suitable to determine water (ascorbic acid, glutathione (GSH) and uric acid) and fat-soluble (all-trans-retinoic acid, all-trans-retinol, α-tocopherol, carotenoids and coenzyme Q10) antioxidants. Data demonstrate that only ascorbic acid is higher in SP than in serum (SP/serum ratio = 4.97 ± 0.88). The other water-soluble antioxidants are equally distributed in the two fluids (GSH SP/serum ratio = 1.14 ± 0.34; uric acid SP/serum ratio = 0.82 ± 0.12). All fat-soluble antioxidants are about 10 times less concentrated in SP than in serum. In donors treated with vitamin C or vitamin E, ascorbic acid and α-tocopherol significantly increased in both fluids. However, the SP/serum ratio of ascorbic acid was 4.15 ± 0.45 before and 3.27 ± 0.39 after treatment, whilst those of α-tocopherol were 0.11 ± 0.03 before and 0.10 ± 0.02 after treatment. The results of this study, by showing the peculiar composition in water- and fat-soluble antioxidants SP, indicate that it is likely that still-unknown mechanisms allow ascorbic acid accumulation in SP against a concentration gradient. SP mainly relies its defenses on water- rather than fat-soluble antioxidants and on the mechanisms ensuring their transfer from serum.

The determination of ascorbic acid and uric acid in human seminal plasma using an HPLC with UV detection

2011 ◽  
Vol 879 (26) ◽  
pp. 2834-2839 ◽  
Author(s):  
Roman Kanďár ◽  
Petra Drábková ◽  
Radek Hampl
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Seminal Plasma ◽  
Uv Detection ◽  
Human Seminal Plasma

Possible Role of Ascorbic Acid in the Oxidative Damage Induced by Inhaled Crystalline Silica Particles

2000 ◽  
Vol 13 (10) ◽  
pp. 971-975 ◽  
Author(s):  
Ivana Fenoglio ◽  
Gianmario Martra ◽  
Salvatore Coluccia ◽  
Bice Fubini
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Silica Particles ◽  
Crystalline Silica

PCB (Aroclor 1254) enhances oxidative damage in rat brain regions: Protective role of ascorbic acid

2007 ◽  
Vol 28 (3) ◽  
pp. 490-498 ◽  
Author(s):  
Prabhu Venkataraman ◽  
Raju Muthuvel ◽  
Gunasekaran Krishnamoorthy ◽  
Arumugam Arunkumar ◽  
Muthusami Sridhar ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Rat Brain ◽  
Brain Regions ◽  
Protective Role ◽  
Aroclor 1254 ◽  
Rat Brain Regions

Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen

1995 ◽  
Vol 10 (1) ◽  
pp. 110-115 ◽  
Author(s):  
J.J. Thiele ◽  
H.J. Freisleben ◽  
J. Fuchs ◽  
F.R. Ochsendorf
Keyword(s):  
Ascorbic Acid ◽  
Seminal Plasma ◽  
Human Seminal Plasma
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