Effects of Epiphytic Load on the Photosynthetic Performance of a Seagrass, Zostera marina, Monitored In Vivo by Chlorophyll Fluorescence Imaging

2009 ◽  
Vol 52 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Min-Hyuk Oh ◽  
Dong Woo Kang ◽  
Tae Hoon Kim ◽  
Yong-Hwan Moon ◽  
Byoung Yong Moon ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Zostera Marina ◽  
Photosynthetic Performance ◽  
Chlorophyll Fluorescence Imaging

Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and resupply on photosynthetic performance of strawberry plants

2014 ◽  
Vol 165 ◽  
pp. 148-155 ◽  
Author(s):  
Júlio Osório ◽  
Maria Leonor Osório ◽  
Pedro José Correia ◽  
Amarilis de Varennes ◽  
Maribela Pestana
Keyword(s):  
Chlorophyll Fluorescence ◽  
Iron Deficiency ◽  
Fluorescence Imaging ◽  
Photosynthetic Performance ◽  
Chlorophyll Fluorescence Imaging ◽  
The Impact

Chlorophyll fluorescence imaging as tool for understanding the impact of fungal diseases on plant performance: a phenomics perspective

2009 ◽  
Vol 36 (11) ◽  
pp. 880 ◽  
Author(s):  
Julie D. Scholes ◽  
Stephen A. Rolfe
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
High Throughput ◽  
Fungal Pathogens ◽  
Photosynthetic Performance ◽  
Plant Performance ◽  
Chlorophyll Fluorescence Imaging ◽  
Infected Leaf ◽  
Experimental Conditions ◽  
The Impact

Chlorophyll fluorescence imaging is a non-invasive, non-destructive means with which to examine the impact of fungal pathogens on the photosynthetic metabolism of host plants. As such, it has great potential for screening purposes in high-throughput phenomics environments. However, there is great diversity in the responses of plants to different plant-fungal pathogens and the choice of suitable experimental conditions and protocols and interpretation of the results requires both preliminary laboratory experiments and an understanding of the biology of the specific plant-pathogen interaction. In this review, we examine the interaction between biotrophic, hemi-biotrophic and necrotrophic fungal pathogens and their hosts to illustrate the extent to which chlorophyll fluorescence imaging can be used to detect the presence of disease before the appearance of visible symptoms, distinguish between compatible and incompatible fungal interactions, identify heterogeneity in photosynthetic performance within the infected leaf and provide insights into the underlying mechanisms. The limitations and challenges of using chlorophyll fluorescence imaging in high throughput screens is discussed.

Screening of growth phases of Antarctic algae and cyanobacteria cultivated on agar plates by chlorophyll fluorescence imaging

2019 ◽  
Vol 9 (2) ◽  
pp. 170-181
Author(s):  
Kristýna Dufková ◽  
Miloš Barták ◽  
Jana Morkusová ◽  
Josef Elster ◽  
Josef Hájek
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Basal Medium ◽  
Photosynthetic Performance ◽  
Chlorophyll Fluorescence Imaging ◽  
Nostoc Commune ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Cultivation Time ◽  
Antarctic Algae

Recently, chlorophyll fluorescence imaging is frequently used non-invasive method to monitor the metabolic state and photosynthetic activities of vascular plants and other autotrophic organisms. In our study, we used the measurements of chlorophyll fluorescence kinetics to follow the development of culture of Antarctic algae (Macrochloris rubrioleum, Zygnema sp.) and cyanobacteria (Hassalia antarctica, Nostoc commune). On the cultures grown on agar plates, Bold´s Basal Medium (BBM), slow Kautsky kinetics supplemented with saturation pulses were measured repeatedly in a week interval. On the kinetics, typical points (OPSMT) were distinguished and species-specific and time of cultivation-dependent differences in shape of the OPSMT kinetics evaluated. We tested sensitivity of various chlorophyll fluorescence parameters to cultivation time on agar plates. In the algae, the most pronounced changes were the decrease in maximum quantum yield of photosystem II (FV/FM) and quenching of basal chlorophyll fluorescence qF0 (M. rubrioleum, Zygnema sp.). In cyanobacteria, chlorophyll fluorescence parameters did not show clear trends with the time of cultivation. F0 quenching (qF0) reached positive values in H. antarctica, while it was negative in N. commune. In both cases, however, qF0 showed an increase with cultivation time. The differences are discussed as well as the potential of the emerging area of the application of chlorophyll fluorescence imaging for evaluation of photosynthetic performance of algal/cyanobacterial cultures on agar plates.

Photoinhibitory printing on leaves, visualised by chlorophyll fluorescence imaging and confocal microscopy, is due to diminished fluorescence from grana

1999 ◽  
Vol 26 (7) ◽  
pp. 717 ◽  
Author(s):  
Barry Osmond ◽  
Owen Schwartz ◽  
Brian Gunning
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Imaging ◽  
Imaging System ◽  
Confocal Microscope ◽  
Chlorophyll Fluorescence Imaging ◽  
Grid Pattern ◽  
Chlorophyll Autofluorescence ◽  
The Stability ◽  
Living Tissues

By analogy with the starch printing technique, it was hypothesised that photoinhibition could be used to print images on leaves that would be invisible to the eye, but easily revealed by chlorophyll fluorescence imaging. We first illustrate the process of chlorophyll fluorescence printing on leaves of the shade plant, Cissus rhombifolia, using photographs of artefacts from starch printing experiments in the laboratory of Molisch. We then use portraits of current leaders in chlorophyll fluorescence research to demonstrate the stability of these images in living tissues. Text printing from microfilm of Ewart’s pioneering studies in photoinhibition shows the resolution of the method with the fixed-focus, portable, imaging system used here. The stability of images, as well as quenching analysis of images and of leaves, suggests that localised photoinactivation, rather than sustained photoprotection, is responsible for the detail displayed by fluorescence printing. Electron micrograph positives of stained thylakoids can be printed to create an illusion of what is imagined to be the source of chlorophyll fluorescence at the membrane level. Individual chloroplasts in adjacent cells under the grid pattern of granal stacks printed on leaves were also examined using a confocal microscope. Compared with chloroplasts in the shaded parts of the grid, those in the photoinactivated parts of the grid show greatly reduced chlorophyll autofluorescence. Moreover, these chloroplasts have lost the localised bright fluorescence from grana. Comparisons of fluorescence yields show that relative chlorophyll autofluorescence from grana observed in the confocal microscope parallels that determined in leaves. Our experiments provide direct visual evidence that fluorescence from grana is lost following photoinactivation of photosystem II in vivo.

In vivo assessing flavonols in white grape berries (Vitis vinifera L. cv. Pinot Blanc) of different degrees of ripeness using chlorophyll fluorescence imaging

2007 ◽  
Vol 34 (12) ◽  
pp. 1092 ◽  
Author(s):  
Sándor Lenk ◽  
Claus Buschmann ◽  
Erhard E. Pfündel
Keyword(s):  
Chlorophyll Fluorescence ◽  
Vitis Vinifera ◽  
Radiation Exposure ◽  
Fluorescence Imaging ◽  
Sugar Content ◽  
Chlorophyll Degradation ◽  
Chlorophyll Fluorescence Imaging ◽  
Vitis Vinifera L ◽  
Grape Berries

Exposed and non-exposed halves of field-grown berries of the white grapevine Vitis vinifera L. cv. Pinot Blanc at various stages of ripeness were analysed using chlorophyll fluorescence imaging. The stage of ripeness was classified by the total sugar concentration which ranged between 120 and 300 g L–1 for the different berries but was similar in the exposed and the non-exposed half of individual berries. Fluorescence was excited in the UV-A and the blue spectral region and detected at red as well as far-red wavelengths. At both emission ranges, UV-excited fluorescence was weak and required correction for the contribution of small false signals. After correction, in vivo UV screening by berry skins was derived from the ratio of UV-A to blue-excited fluorescence intensities, and a relationship between in vivo UV screening and flavonol quantity was established: the quantity of flavonols was determined by spectral analysis of extracted phenolics. Significantly high flavonol concentrations, and effective in vivo UV screening, were detected in most exposed half-berries at sugar concentrations higher than 200 g L–1 but not in non-exposed samples. This suggests that radiation-exposure conditions determine flavonol synthesis. Based on the absence of flavonol accumulation in exposed half-berries with sugar concentrations smaller than 200 g L–1, however, it is suggested that berries need to arrive at an advanced stage of ripeness before responding to radiation-exposure by synthesising large amounts of UV-protecting flavonols. Chlorophyll degradation, which was followed by blue-excited intensities of far-red fluorescence, progressed in parallel with increasing sugar content suggesting that chlorophyll degradation is associated with berry ripening. In addition, exposure to sunlight appeared to slightly stimulate chlorophyll decay.

Detecting crop population growth using chlorophyll fluorescence imaging

2017 ◽  
Vol 56 (35) ◽  
pp. 9762 ◽  
Author(s):  
Heng Wang ◽  
Xiangjie Qian ◽  
Lan Zhang ◽  
Sailong Xu ◽  
Haifeng Li ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Population Growth ◽  
Fluorescence Imaging ◽  
Chlorophyll Fluorescence Imaging
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