Recombinant Lactococcus Lactis Displaying Omp31 Antigen of Brucella melitensis Can Induce an Immunogenic Response in BALB/c Mice

2020 ◽  
Author(s):  
Hoda Shirdast ◽  
Fatemeh Ebrahimzadeh ◽  
Amir Hossein Taromchi ◽  
Yousef Mortazavi ◽  
Abdolreza Esmaeilzadeh ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Brucella Melitensis ◽  
Recombinant Lactococcus Lactis ◽  
Immunogenic Response

scholarly journals Induction of Immunogenic Response in BALB/c Mice by Live and Killed Form of Recombinant Lactococcus lactis Displaying EG95 of Echinococcus granulosus

2021 ◽  
Vol 25 (4) ◽  
pp. 284-296
Author(s):  
Fatemeh Ebrahimzadeh ◽  
Hoda Shirdast ◽  
Amirhossein Taromchi ◽  
Yeganeh Talebkhan ◽  
Ali Haniloo ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Echinococcus Granulosus ◽  
Recombinant Lactococcus Lactis ◽  
Immunogenic Response

scholarly journals Oral immunization of a non-recombinant Lactococcus lactis surface displaying influenza hemagglutinin 1 (HA1) induces mucosal immunity in mice

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187718 ◽  
Author(s):  
Pui-Fong Jee ◽  
Vunjia Tiong ◽  
Meng-Hooi Shu ◽  
Jing-Jing Khoo ◽  
Won Fen Wong ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Mucosal Immunity ◽  
Oral Immunization ◽  
Influenza Hemagglutinin ◽  
Recombinant Lactococcus Lactis

scholarly journals Oral vaccination of mice against tetanus with recombinant Lactococcus lactis

1997 ◽  
Vol 15 (7) ◽  
pp. 653-657 ◽  
Author(s):  
Karen Robinson ◽  
Lisa M. Chamberlain ◽  
Karin M. Schofield ◽  
Jeremy M. Wells ◽  
Richard W.F. Le Page
Keyword(s):  
Lactococcus Lactis ◽  
Oral Vaccination ◽  
Recombinant Lactococcus Lactis

scholarly journals The recombinant Lactococcus lactis oral vaccine induces protection against C. difficile spore challenge in a mouse model

Vaccine ◽  
2015 ◽  
Vol 33 (13) ◽  
pp. 1586-1595 ◽  
Author(s):  
Shanguang Guo ◽  
Weiwei Yan ◽  
Sean P. McDonough ◽  
Nengfeng Lin ◽  
Katherine J. Wu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Lactococcus Lactis ◽  
Oral Vaccine ◽  
Recombinant Lactococcus Lactis

Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env

Blood ◽  
2003 ◽  
Vol 102 (1) ◽  
pp. 223-228 ◽  
Author(s):  
Ke-Qin Xin ◽  
Yuka Hoshino ◽  
Yoshihiko Toda ◽  
Shizunobu Igimi ◽  
Yoshitsugu Kojima ◽  
...  
Keyword(s):  
Immune Responses ◽  
Lactococcus Lactis ◽  
Potential Role ◽  
Protective Efficacy ◽  
Oral Immunization ◽  
Vaccine Strategy ◽  
Regional Lymph Nodes ◽  
Viral Loads ◽  
Recombinant Lactococcus Lactis ◽  
Further Development

Abstract This study investigates whether genetically modified orally administered Lactococcus lactis (L lactis) could be used as an HIV vaccine. L lactis is immunogenic and extremely safe when delivered orally. We created a recombinant L lactis vector expressing the envelope protein of HIV on its cell surface. Oral immunization with this vector induced high levels of HIV-specific serum IgG and fecal IgA antibodies. Cell-mediated immune responses also were generated in both the regional lymph nodes and the spleen. Dendritic cells are readily infected by L lactis and appear to play a potential role in mediating the development of these immune responses. The protective efficacy of this vaccine strategy was demonstrated by challenging mice intraperitoneally with an HIV Env–expressing vaccinia virus. Their viral loads were 350-fold lower than those of control mice. These findings support the further development of L lactis–based HIV vaccines. (Blood. 2003; 102:223-228)

Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine against brucellosis

2020 ◽  
Vol 66 (1) ◽  
pp. 39-45
Author(s):  
Marzieh Rezaei ◽  
Mohammad Rabbani Khorasgani ◽  
Sayyed Hamid Zarkesh Esfahani ◽  
Rahman Emamzadeh ◽  
Hamid Abtahi
Keyword(s):  
Fusion Protein ◽  
Lactococcus Lactis ◽  
Fusion Gene ◽  
Brucella Melitensis ◽  
Expression System ◽  
Interleukin 2 ◽  
Cell Factory ◽  
Promising Alternative ◽  
Alternative Expression ◽  
Restriction Sites

The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with appropriate restriction sites and subcloned in pAMJ2008 expression vector digested with the same enzymes. The purified recombinant constructed pAMJ-rOmp-IL2 was introduced into L. lactis subsp. cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2 fusion protein was investigated. This study reports the construction of a recombinant L. lactis expressing the Omp16-IL2 fusion protein as an oral Lactococcus-based vaccine, as compared with commonly used live attenuated vaccines, for future studies against brucellosis.

Surface Display of Heterologous β-Galactosidase in Food-Grade Recombinant Lactococcus lactis

2018 ◽  
Vol 75 (10) ◽  
pp. 1362-1371 ◽  
Author(s):  
Supeng Yin ◽  
Hongbin Zhu ◽  
Mengyu Shen ◽  
Gang Li ◽  
Shuguang Lu ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Surface Display ◽  
Food Grade ◽  
Recombinant Lactococcus Lactis
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