In Vitro and In Vivo Evaluation of Lacticaseibacillus rhamnosus GG and Bifidobacterium lactis Bb12 Against Avian Pathogenic Escherichia coli and Identification of Novel Probiotic-Derived Bioactive Peptides

2021 ◽  
Author(s):  
Dipak Kathayat ◽  
Gary Closs ◽  
Yosra A. Helmy ◽  
Loic Deblais ◽  
Vishal Srivastava ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bioactive Peptides ◽  
Avian Pathogenic Escherichia Coli ◽  
In Vivo Evaluation ◽  
Bifidobacterium Lactis ◽  
Pathogenic Escherichia Coli

scholarly journals Sensitization of avian pathogenic Escherichia coli to amoxicillin in vitro and in vivo in the presence of surfactin

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222413
Author(s):  
Jiaxu Liu ◽  
Xu Wang ◽  
Weiqi Shi ◽  
Zhuoyu Qian ◽  
Yongqiang Wang
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

The YfcO fimbriae gene enhances adherence and colonization abilities of avian pathogenic Escherichia coli in vivo and in vitro

2016 ◽  
Vol 100 ◽  
pp. 56-61 ◽  
Author(s):  
Yaxin Li ◽  
Haojin Wang ◽  
Jianluan Ren ◽  
Ling Chen ◽  
Xiangkai Zhuge ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro

2018 ◽  
Vol 214 ◽  
pp. 28-36 ◽  
Author(s):  
Renu Verma ◽  
Thaís Cabrera Galvão Rojas ◽  
Renato Pariz Maluta ◽  
Janaína Luisa Leite ◽  
Gerson Nakazato ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hypothetical Protein ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals ClbG in Avian Pathogenic Escherichia coli Contributes to Meningitis Development in a Mouse Model

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 546
Author(s):  
Peili Wang ◽  
Jiaxiang Zhang ◽  
Yanfei Chen ◽  
Haoran Zhong ◽  
Heng Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Cycle ◽  
Clinical Symptoms ◽  
Bacterial Colonization ◽  
Eukaryotic Cell ◽  
Avian Pathogenic Escherichia Coli ◽  
Cycle Arrest ◽  
Pathogenic Escherichia Coli

Colibactin is a complex secondary metabolite that leads to genotoxicity that interferes with the eukaryotic cell cycle. It plays an important role in many diseases, including neonatal mouse sepsis and meningitis. Avian pathogenic Escherichia coli (APEC) is responsible for several diseases in the poultry industry and may threaten human health due to its potential zoonosis. In this study, we confirmed that clbG was necessary for the APEC XM strain to produce colibactin. The deletion of clbG on APEC XM contributed to lowered γH2AX expression, no megalocytosis, and no cell cycle arrest in vitro. None of the 4-week Institute of Cancer Research mice infected with the APEC XM ΔclbG contracted meningitis or displayed weakened clinical symptoms. Fewer histopathological lesions were observed in the APEC XM ΔclbG group. The bacterial colonization of tissues and the relative expression of cytokines (IL-1β, IL-6, and TNF-α) in the brains decreased significantly in the APEC XM ΔclbG group compared to those in the APEC XM group. The tight junction proteins (claudin-5, occludin, and ZO-1) were not significantly destroyed in APEC XM ΔclbG group in vivo and in vitro. In conclusion, clbG is necessary for the synthesis of the genotoxin colibactin and affects the development of APEC meningitis in mice.

Serological diversity, molecular characterisation and antimicrobial sensitivity of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in Kashmir, India

2019 ◽  
Vol 59 (2) ◽  
pp. 338
Author(s):  
S. N. Magray ◽  
S. A. Wani ◽  
Z. A. Kashoo ◽  
M. A. Bhat ◽  
S. Adil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Significant Difference ◽  
Virulence Gene Profile

The present study has determined the serological diversity, virulence-gene profile and in vitro antibiogram of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in India suspected to have died of colibacillosis. The virulence-gene profile of APEC was compared with that of the Escherichia coli isolates from faeces of apparently healthy chickens, called avian faecal E. coli (AFEC). In total, 90 representative isolates of APEC and 63 isolates of AFEC were investigated in the present study. The APEC were typed into 19 serogroups, while some isolates were rough and could not be typed. Most prevalent serogroup was O2 (24.44%). Among the eight virulence genes studied, the prevalence of seven genes (iss, iucD, tsh, cva/cvi, irp2, papC and vat) was significantly higher in APEC than in AFEC isolates. However, there was no significant difference between APEC and AFEC isolates for possession of astA gene. The most frequent gene detected among the two groups of organisms was iss, which was present in 98.88% and 44.44% of APEC and AFEC isolates respectively. The in vitro antibiogram showed that the majority (96.6%) of APEC isolates were resistant to tetracycline, while 82.2% were resistant to cephalexin, 78.8% to cotrimoxazole, 68.8% to streptomycin and 63.3% to ampicillin. However, most of them (84.45%) were sensitive to gentamicin. Thus, it is concluded that APEC from the broiler chickens carried putative virulence genes that attributed to their pathogenicity. Furthermore, the majority of APEC isolates were found to be multi-drug resistant, which, in addition to leading treatment failures in poultry, poses a public health threat.

Biofilm Formation by Avian Pathogenic Escherichia coli is Not Related to In Vivo Pathogenicity

2018 ◽  
Vol 76 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Suelle V. Rodrigues ◽  
Vanessa Laviniki ◽  
Karen A. Borges ◽  
Thales Q. Furian ◽  
Hamilton L. S. Moraes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Fimbria-Encoding GeneyadCHas a Pleiotropic Effect on Several Biological Characteristics and Plays a Role in Avian Pathogenic Escherichia coli Pathogenicity

2015 ◽  
Vol 84 (1) ◽  
pp. 187-193 ◽  
Author(s):  
Renu Verma ◽  
Thaís Cabrera Galvão Rojas ◽  
Renato Pariz Maluta ◽  
Janaína Luisa Leite ◽  
Livia Pilatti Mendes da Silva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Soft Agar ◽  
Pleiotropic Effect ◽  
Biological Characteristics ◽  
Wild Type ◽  
Content Type ◽  
Pathogenic Escherichia Coli

The extraintestinal pathogen termed avian pathogenicEscherichia coli(APEC) is known to cause colibacillosis in chickens. The molecular basis of APEC pathogenesis is not fully elucidated yet. In this work, we deleted a component of the Yad gene cluster (yadC) in order to understand the role of Yad in the pathogenicity of the APEC strain SCI-07.In vitro, the transcription level ofyadCwas upregulated at 41°C and downregulated at 22°C. TheyadCexpressionin vivowas more pronounced in lungs than in spleen, suggesting a role in the early steps of the infection. Chicks infected with the wild-type and mutant strains presented, respectively, 80% and 50% mortality rates. The ΔyadCstrain presented a slightly decreased ability to adhere to HeLa cells with or without thed-mannose analog compared with the wild type. Real-time PCR (RT-PCR) assays showed thatfimHwas downregulated (P< 0.05) andcsgAandecpAwere slightly upregulated in the mutant strain, showing thatyadCmodulates expression of other fimbriae. Bacterial internalization studies showed that the ΔyadCstrain had a lower number of intracellular bacteria recovered from Hep-2 cells and HD11 cells than the wild-type strain (P< 0.05). Motility assays in soft agar demonstrated that the ΔyadCstrain was less motile than the wild type (P< 0.01). Curiously, flagellum-associated genes were not dramatically downregulated in the ΔyadCstrain. Taken together, the results show that the fimbrial adhesin Yad contributes to the pathogenicity and modulates different biological characteristics of the APEC strain SCI-07.

scholarly journals In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system

2020 ◽  
Vol 367 (14) ◽  
Author(s):  
Sofia Chioccioli ◽  
Patrizia Bogani ◽  
Sara Del Duca ◽  
Lara Mitia Castronovo ◽  
Alberto Vassallo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hybrid System ◽  
De Novo ◽  
Glycerol Phosphate ◽  
In Vivo Evaluation ◽  
Igp Synthase ◽  
Imidazole Glycerol Phosphate ◽  
Two Hybrid

ABSTRACT Histidine biosynthesis is one of the most characterized metabolic routes for its antiquity and its central role in cellular metabolism; indeed, it represents a cross-road between nitrogen metabolism and de novo synthesis of purines. This interconnection is due to the activity of imidazole glycerol phosphate synthase, a heterodimeric enzyme constituted by the products of two his genes, hisH and hisF, encoding a glutamine amidotransferase and a cyclase, respectively. Despite their interaction was suggested by several in vitro experiments, their in vivo complex formation has not been demonstrated. On the contrary, the analysis of the entire Escherichia coli interactome performed using the yeast two hybrid system did not suggest the in vivo interaction of the two IGP synthase subunits. The aim of this study was to demonstrate the interaction of the two proteins using the Bacterial Adenylate Cyclase Two-Hybrid (BACTH) system. Data obtained demonstrated the in vivo interaction occurring between the proteins encoded by the E. coli hisH and hisF genes; this finding might also open the way to pharmaceutical applications through the design of selective drugs toward this enzyme.

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