scholarly journals In vitro antimicrobial activity of Cannabis sativa (Hemp) extracts against avian pathogenic Escherichia coli (APEC) isolated from Broilers Chicken

2020 ◽  
Vol 5 (5) ◽  
pp. 164-171
Author(s):  
Yunusa Umar Abubakar ◽  
Dalha Wada Taura ◽  
Aminu Binta Muhammad ◽  
Tikur Shehu Ibrahim ◽  
Mohammad Umar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Cannabis Sativa ◽  
Avian Pathogenic Escherichia Coli ◽  
In Vitro Antimicrobial Activity ◽  
Pathogenic Escherichia Coli

Serological diversity, molecular characterisation and antimicrobial sensitivity of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in Kashmir, India

2019 ◽  
Vol 59 (2) ◽  
pp. 338
Author(s):  
S. N. Magray ◽  
S. A. Wani ◽  
Z. A. Kashoo ◽  
M. A. Bhat ◽  
S. Adil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Significant Difference ◽  
Virulence Gene Profile

The present study has determined the serological diversity, virulence-gene profile and in vitro antibiogram of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in India suspected to have died of colibacillosis. The virulence-gene profile of APEC was compared with that of the Escherichia coli isolates from faeces of apparently healthy chickens, called avian faecal E. coli (AFEC). In total, 90 representative isolates of APEC and 63 isolates of AFEC were investigated in the present study. The APEC were typed into 19 serogroups, while some isolates were rough and could not be typed. Most prevalent serogroup was O2 (24.44%). Among the eight virulence genes studied, the prevalence of seven genes (iss, iucD, tsh, cva/cvi, irp2, papC and vat) was significantly higher in APEC than in AFEC isolates. However, there was no significant difference between APEC and AFEC isolates for possession of astA gene. The most frequent gene detected among the two groups of organisms was iss, which was present in 98.88% and 44.44% of APEC and AFEC isolates respectively. The in vitro antibiogram showed that the majority (96.6%) of APEC isolates were resistant to tetracycline, while 82.2% were resistant to cephalexin, 78.8% to cotrimoxazole, 68.8% to streptomycin and 63.3% to ampicillin. However, most of them (84.45%) were sensitive to gentamicin. Thus, it is concluded that APEC from the broiler chickens carried putative virulence genes that attributed to their pathogenicity. Furthermore, the majority of APEC isolates were found to be multi-drug resistant, which, in addition to leading treatment failures in poultry, poses a public health threat.

scholarly journals Sensitization of avian pathogenic Escherichia coli to amoxicillin in vitro and in vivo in the presence of surfactin

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222413
Author(s):  
Jiaxu Liu ◽  
Xu Wang ◽  
Weiqi Shi ◽  
Zhuoyu Qian ◽  
Yongqiang Wang
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

2020 ◽  
Vol 8 (8) ◽  
pp. 1135
Author(s):  
Otun Saha ◽  
M. Nazmul Hoque ◽  
Ovinu Kibria Islam ◽  
Md. Mizanur Rahaman ◽  
Munawar Sultana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Avian Pathogenic Escherichia Coli ◽  
E Coli ◽  
Molecular Approaches ◽  
Clade B ◽  
Pathogenic Escherichia Coli ◽  
Resistance Patterns

The avian pathogenic Escherichia coli (APEC) strains are the chief etiology of colibacillosis worldwide. The present study investigated the circulating phylotypes, existence of virulence genes (VGs), and antimicrobial resistance (AMR) in 392 APEC isolates, obtained from 130 samples belonged to six farms using both phenotypic and PCR-based molecular approaches. Congo red binding (CRB) assay confirmed 174 APEC isolates which were segregated into ten, nine, and eight distinct genotypes by RAPD assay (discriminatory index, DI = 0.8707), BOX-PCR (DI = 0.8591) and ERIC-PCR (DI = 0.8371), respectively. The combination of three phylogenetic markers (chuA, yjaA and DNA fragment TspE4.C2) classified APEC isolates into B23 (37.36%), A1 (33.91%), D2 (11.49%), B22 (9.20%), and B1 (8.05%) phylotypes. Majority of the APEC isolates (75–100%) harbored VGs (ial, fimH, crl, papC, and cjrC). These VGs (papC and cjrC) and phylotypes (D2 and B2) of APEC had significant (p = 0.004) association with colibacillosis. Phylogenetic analysis showed two distinct clades (clade A and clade B) of APEC, where clade A had 98–100% similarity with E. coli APEC O78 and E. coli EHEC strains, and clade B had closest relationship with E. coli O169:H41 strain. Interestingly, phylogroups B2 and D2 were found in the APEC strains of both clades, while the strains from phylogroups A1 and B1 were found in clade A only. In this study, 81.71% of the isolates were biofilm formers, and possessed plasmids of varying ranges (1.0 to 54 kb). In vitro antibiogram profiling revealed that 100% isolates were resistant to ≥3 antibiotics, of which 61.96%, 55.24%, 53.85%, 51.16% and 45.58% isolates in phylotypes B1, D2, B22, B23, and A1, respectively, were resistant to these antimicrobials. The resistance patterns varied among different phylotypes, notably in phylotype B22, showing the highest resistance to ampicillin (90.91%), nalidixic acid (90.11%), tetracycline (83.72%), and nitrofurantoin (65.12%). Correspondence analysis also showed significant correlation among phylotypes with CRB (p = 0.008), biofilm formation (p = 0.02), drug resistance (p = 0.03), and VGs (p = 0.06). This report demonstrated that B2 and A1 phylotypes are dominantly circulating APEC phylotypes in Bangladesh; however, B2 and D2 are strongly associated with the pathogenicity. A high prevalence of antibiotic-resistant APEC strains from different phylotypes suggest the use of organic antimicrobial compounds, and/or metals, and the rotational use of antibiotics in poultry farms in Bangladesh.

In Vitro Antimicrobial Activity Study of Some Organotin(IV) Chlorobenzoates against Staphylococcus aureus and Escherichia coli ‎

2021 ◽  
Vol 11 (2) ◽  
pp. 17-22
Author(s):  
Samsuar Samsuar ◽  
Wasinton Simanjuntak ◽  
Hardoko Insan Qudus ◽  
Yandri Yandri ◽  
Dian Herasari ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
In Vitro Antimicrobial Activity

scholarly journals Antimicrobial in vitro activities of condensed tannin extracts on avian pathogenic Escherichia coli

2020 ◽  
Vol 70 (3) ◽  
pp. 165-172 ◽  
Author(s):  
M.M. Dakheel ◽  
F.A.H. Alkandari ◽  
I. Mueller‐Harvey ◽  
M.J. Woodward ◽  
C. Rymer
Keyword(s):  
Escherichia Coli ◽  
Condensed Tannin ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

The YfcO fimbriae gene enhances adherence and colonization abilities of avian pathogenic Escherichia coli in vivo and in vitro

2016 ◽  
Vol 100 ◽  
pp. 56-61 ◽  
Author(s):  
Yaxin Li ◽  
Haojin Wang ◽  
Jianluan Ren ◽  
Ling Chen ◽  
Xiangkai Zhuge ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

In vitro antimicrobial activity of six Ethiopian medicinal plants against Staphylococcus aureus, Escherichia coli and Candida albicans

2020 ◽  
Vol 36 ◽  
pp. 101121
Author(s):  
Nigus Zegeye Gishen ◽  
Solomon Taddese ◽  
Tizazu Zenebe ◽  
Kassahun Dires ◽  
Abebe Tedla ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Medicinal Plants ◽  
In Vitro Antimicrobial Activity
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