Development of microsatellite markers for the critically endangered frog Telmatobufo bullocki and cross-species amplification in two related species

2014 ◽  
Vol 6 (4) ◽  
pp. 883-884
Author(s):  
Virginia Moreno-Puig ◽  
Yeşerin Yildirim ◽  
Dianne H. Brunton
Keyword(s):  
Microsatellite Markers ◽  
Related Species ◽  
Critically Endangered

scholarly journals Development of microsatellite markers for the critically endangered conifer Glyptostrobus pensilis (Cupressaceae) using transcriptome data

2019 ◽  
Vol 68 (1) ◽  
pp. 41-44 ◽  
Author(s):  
Xin-Yu Li ◽  
Xue-Ying Lin ◽  
Markus Ruhsam ◽  
Lu Chen ◽  
Xing-Tong Wu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Related Species ◽  
Population Genetic ◽  
Critically Endangered ◽  
Transcriptome Data ◽  
Genetic Studies ◽  
Chinese Populations ◽  
Expected Heterozygosity ◽  
Ssr Loci ◽  
Hardy Weinberg Equilibrium

Abstract Glyptostrobus pensilis (Cupressaceae) is a critically endangered conifer which occurs in China, Laos, and Vietnam where it is only known from a few populations. Here we aim to develop microsatellite markers which can be used to study the genetic variation within this species. Using transcriptome data we tested 170 SSR loci for polymorphism in 16 samples. Twenty-three loci were polymorphic and selected for the genetic analysis of 83 individuals from three Chinese populations. The number of alleles per locus and population ranged from one to eight, the observed and expected heterozygosity from Ho = 0.00-1.00 and He = 0.00-0.83, respectively. Fifteen loci deviated from Hardy-Weinberg equilibrium in at least one of the three populations. The majority of loci could also be successfully amplified in four related species, namely Cryptomeria fortunei, Taxodium distichum, Taxodium ascendens and Cunninghamia lanceolata. These developed microsatellites are suitable for population genetic studies of Glyptostrobus pensilis and related species.

Microsatellite Marker: Importance and Implications of Cross-genome Analysis for Finger Millet (Eleusine coracana (L.) Gaertn)

2020 ◽  
Vol 9 (3) ◽  
pp. 160-170
Author(s):  
Thumadath P.A. Krishna ◽  
Maharajan Theivanayagam ◽  
Gurusunathan V. Roch ◽  
Veeramuthu Duraipandiyan ◽  
Savarimuthu Ignacimuthu
Keyword(s):  
Molecular Marker ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Genome Analysis ◽  
Related Species ◽  
Finger Millet ◽  
Crop Improvement ◽  
Human Beings ◽  
Closely Related Species ◽  
Genome Amplification

Finger millet is a superior staple food for human beings. Microsatellite or Simple Sequence Repeat (SSR) marker is a powerful tool for genetic mapping, diversity analysis and plant breeding. In finger millet, microsatellites show a higher level of polymorphism than other molecular marker systems. The identification and development of microsatellite markers are extremely expensive and time-consuming. Only less than 50% of SSR markers have been developed from microsatellite sequences for finger millet. Therefore, it is important to transfer SSR markers developed for related species/genus to finger millet. Cross-genome transferability is the easiest and cheapest method to develop SSR markers. Many comparative mapping studies using microsatellite markers clearly revealed the presence of synteny within the genomes of closely related species/ genus. Sufficient homology exists among several crop plant genomes in the sequences flanking the SSR loci. Thus, the SSR markers are beneficial to amplify the target regions in the finger millet genome. Many SSR markers were used for the analysis of cross-genome amplification in various plants such as Setaria italica, Pennisetum glaucum, Oryza sativa, Triticum aestivum, Zea mays and Hordeum vulgare. However, there is very little information available about cross-genome amplification of these markers in finger millet. The only limited report is available for the utilization of cross-genome amplified microsatellite markers in genetic analysis, gene mapping and other applications in finger millet. This review highlights the importance and implication of microsatellite markers such as genomic SSR (gSSR) and Expressed Sequence Tag (EST)-SSR in cross-genome analysis in finger millet. Nowadays, crop improvement has been one of the major priority areas of research in agriculture. The genome assisted breeding and genetic engineering plays a very crucial role in enhancing crop productivity. The rapid advance in molecular marker technology is helpful for crop improvement. Therefore, this review will be very helpful to the researchers for understanding the importance and implication of SSR markers in closely related species.

Microsatellite markers for the endangered shrub Myrceugenia rufa (Myrtaceae) and three closely related species

2014 ◽  
Vol 6 (3) ◽  
pp. 773-775 ◽  
Author(s):  
Fernanda Pérez ◽  
C. Carla Irarrázabal ◽  
Michel Cossio ◽  
Gioconda Peralta ◽  
Ricardo Segovia ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Related Species ◽  
Closely Related Species

Fritillaria arsusiana (Lilieae, Liliaceae), a new species from southern Anatolia

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 149-159
Author(s):  
HASAN YILDIRIM ◽  
MEHTAP TEKŞEN
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Related Species ◽  
Conservation Status ◽  
Flower Colour ◽  
Identification Key ◽  
Critically Endangered ◽  
Diagnostic Characters ◽  
A New Species ◽  
Shape And Size

In this study, Fritillaria arsusiana (Liliaceae) is described and illustrated as a new species from Hatay province in southern Anatolia, Turkey. It is related to F. amana and F. hermonis by habitus features and broadly campanulate flowers, but differs mainly by its bulb shape and size, smaller leaf and flower features, and flower colour. It has also been morphologically compared with F. wendelboi, F. pinardii, and F. latakiensis. The detailed description, diagnostic characters, original photographs, geographical distribution, habitat and phenology, etymology, conservation status, and identification key of the new and the related species are presented in this study. IUCN conservation status of F. arsusiana is suggested as Critically Endangered (CR).

Microsatellite markers help to assess genetic diversity among Opuntia ficus indica cultivated genotypes and their relation with related species

2010 ◽  
Vol 290 (1-4) ◽  
pp. 85-97 ◽  
Author(s):  
Marco Caruso ◽  
Sergio Currò ◽  
Giuseppina Las Casas ◽  
Stefano La Malfa ◽  
Alessandra Gentile
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Related Species ◽  
Opuntia Ficus Indica

Kielmeyera ferruginosa (Calophyllaceae), a new species from the Atlantic Forest, southern Bahia, Brazil

Phytotaxa ◽  
2015 ◽  
Vol 221 (3) ◽  
pp. 288 ◽  
Author(s):  
Amanda Pricilla Batista Santos ◽  
Rafaela Jorge Trad ◽  
Fabio Da Silva Do Espírito Santo ◽  
Maria do Carmo Estanislau Do Amaral ◽  
Alessandro Rapini
Keyword(s):  
New Species ◽  
Atlantic Forest ◽  
Related Species ◽  
The State ◽  
The Other ◽  
Critically Endangered ◽  
Abaxial Surface ◽  
Southern Bahia ◽  
A New Species ◽  
Flooded Areas

Kielmeyera ferruginosa, a new species of Calophyllaceae from the Atlantic Forest of southern Bahia, is here described and illustrated. The species is critically endangered and can be easily distinguished from other Kielmeyera species by the rusty coloration of the trunk and branches, orange-colored latex, and the occurrence in flooded areas. It differs from the other species of the section Prolifera by the leaves with secondary veins relatively more distant from each other and prominent on the abaxial surface. Diagnostic characters and the state of conservation of the new species are discussed, and an identification key for K. ferruginosa and related species from series Rupestres and Neglectae is provided.

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