scholarly journals Exploration of miRNA-mediated fertility regulation network of cytoplasmic male sterility during flower bud development in soybean

3 Biotech ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xianlong Ding ◽  
Hao Zhang ◽  
Hui Ruan ◽  
Yanwei Li ◽  
Linfeng Chen ◽  
...  
2018 ◽  
Author(s):  
Ding Xianlong ◽  
Zhang Hao ◽  
Ruan Hui ◽  
Li Yanwei ◽  
Chen Linfeng ◽  
...  

ABSTRACTCytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. Here, two small RNA libraries and two degradome libraries were constructed from the flower buds of the soybean CMS line NJCMS1A and its restorer (Rf) line NJCMS1C. Following high-throughput sequencing, 558 known miRNAs, 103 novel miRNAs on the other arm of known pre-miRNAs, 10 novel miRNAs, and a number of base-edited miRNAs were identified. Among the identified miRNAs, 76 differentially expressed miRNAs were discovered with greater than two-fold changes between NJCMS1A and NJCMS1C. By degradome analysis, a total of 466 distinct transcripts targeted by 200 miRNAs and 122 distinct transcripts targeted by 307 base-edited miRNAs were detected. Further integrated analysis of transcriptome and small RNA found some miRNAs and their targets’ expression patterns showing a negative correlation, such as miR156b-GmSPL and miR4413b-GmPPR. Previous reports showed that these targets might be related to flower bud development, suggesting that miRNAs might act as regulators of soybean CMS fertility. These findings may provide a better understanding of the miRNA-mediated regulatory networks in CMS mechanisms of soybean.


2018 ◽  
Vol 70 (3) ◽  
pp. 937-948 ◽  
Author(s):  
Faline D M Plantenga ◽  
Sara Bergonzi ◽  
José A Abelenda ◽  
Christian W B Bachem ◽  
Richard G F Visser ◽  
...  

1998 ◽  
Vol 123 (4) ◽  
pp. 586-591 ◽  
Author(s):  
Kiyoshi Ohkawa ◽  
Hyeon-Hye Kim ◽  
Emiko Nitta ◽  
Yukinori Fukazawa

Leucocoryne, a native to Chile, has violet, blue, or white flowers and is increasing in popularity as a cut flower. The effects of storage temperature and duration on flower bud development, shoot emergence, and anthesis were investigated. Bulbs stored at 20 to 30 °C for 22 weeks produced 3.4 flower stems per bulb between March and April. Bulbs stored at 20 °C flowered earliest, followed by those stored at 25 °C. Bulbs stored at 30 °C flowered last. After 16 weeks of storage at 20 °C, a further 2 weeks dry storage at 15 °C before planting resulted in 1 month earlier flowering with no reduction of the number of flowering stems. As dry storage at 20 °C increased to 11 months, the time to emergence and flowering decreased. After dry storage at 20 °C for 12 months, the primary flower stems aborted and secondary stems then developed. Secondary and tertiary flower stems tend to commence flower bud development after the flower bud on the primary flower stem has reached the gynoecium or anther and ovule stage of initiation.


1999 ◽  
Vol 77 (2) ◽  
pp. 262-268
Author(s):  
Michael J Sumner ◽  
William R Remphrey ◽  
Richard Martin

A relationship was developed between phenological stages of inflorescence expansion and the internal development of pollen within the anther of Amelanchier alnifolia Nutt. flowers. The major microscopic events associated with microsporogenesis and microgametogenesis were correlated with seven stages of external inflorescence development in both natural buds and those forced from dormancy in different concentrations of gibberellin at various times of the year. In fall and early spring, it was found that gibberellin at a concentration of 2.5 mg/L forced buds to produce inflorescences that most resembled those from natural field populations. However, it was not possible to force flower buds to develop all the way to anthesis. Flower bud development stopped when the pollen was at the binucleate stage. Despite this limitation, the ability to force buds increases the time frame for the study of many aspects of the reproductive biology of A. alnifolia.Key words: microsporogenesis, microgametogenesis, gibberellin, GA, flowering.


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