scholarly journals Optimization of cell suspension culture of transformed and untransformed lettuce for the enhanced production of secondary metabolites and their pharmaceutical evaluation

3 Biotech ◽  
2019 ◽  
Vol 9 (9) ◽  
Author(s):  
Hammad Ismail ◽  
Sania Sabahat Kayani ◽  
Sadaf Ilyas Kayani ◽  
Bushra Mirza ◽  
Mohammad Tahir Waheed
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Enhanced Production

Effects of different factors on friable callus induction and establishment of cell suspension culture of Hovenia dulcis (Rhamnaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Ivan Gonçalves Ribeiro ◽  
Tatiana Carvalho de Castro ◽  
Marsen Garcia Pinto Coelho ◽  
Norma Albarello
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Friable Callus ◽  
Weight Analysis ◽  
Hovenia Dulcis

Abstract Medicinal plants are an important therapeutic option for a large share of the world’s population. To establish an in vitro culture system for the production of secondary metabolites from Hovenia dulcis, we studied the effect of auxins, cytokinins, absence of light, and silver nitrate on the development of friable callus. Callus cultures were established for the first time and used to obtain cell suspension cultures. Supplementation with KIN (Kinetin) produced calli with both compact and friable areas, while the addition of TDZ (Thidiazuron) only produced compact callus. The maintenance of cultures in the dark induced a slight enhancement on friability when the auxin PIC (Picloram) was present in the culture medium. The addition of silver nitrate promoted the formation of friable calli. Dry weight analysis showed no significant differences in biomass growth, and, therefore, 2.0 mg.L-1 was considered the most suitable treatment. The presence of silver nitrate was not required for the establishment of cell suspension cultures. Dry weight analysis of cell suspensions showed higher biomass production in the absence of silver nitrate. PIC promoted 100% of cell suspension culture formation in the absence of silver nitrate, and higher biomass production was observed with the lowest concentration (0.625 mg.L-1). No morphological differences were observed among the different concentrations of PIC. Phytochemical screening showed the presence of saponins, flavonoids, flavonols and catechins in the extracts obtained from H. dulcis calli. These results show that the cell cultures herein established are potential sources for the production of H. dulcis secondary metabolites of medicinal interest.

scholarly journals Enhanced production of phenolic acids in cell suspension culture of Salvia leriifolia Benth. using growth regulators and sucrose

2018 ◽  
Vol 70 (2) ◽  
pp. 741-750 ◽  
Author(s):  
Masoomeh Modarres ◽  
Sedigheh Esmaeilzadeh Bahabadi ◽  
Mohammad Ehsan Taghavizadeh Yazdi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Enhanced Production

scholarly journals Sink ? Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

2016 ◽  
Vol 26 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Vandita Billore ◽  
Lalit Khatediya ◽  
Monica Jain
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Cell Mass ◽  
Scale Up ◽  
Light Conditions ◽  
Yellow Light ◽  
Celastrus Paniculatus

Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high?value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids (91.69 ?g/1750 ml) and phenols (70.59 ?g/1750 ml) was obtained during 30 days of culture under yellow light conditions. The production was remarkably enhanced by 100?folds (5.29 to 48.21 ?g; Fig. 2) for alkaloids and 70?folds (1.73 to 46.33 ?g; Fig. 3) for phenols, from zero days to 30?day?old culture phase. Hence, strategic implementation of monochromatic lights holds great promises for controlled commercial production of myriads of valuable secondary metabolites from plant cell cultures of important medicinal plants. Present work contributes to the first reports where continuous, enhanced, multifold yield of important secondary metabolites were obtained from cell suspension culture of Celasrus paniculatus an endangered medicinal plant.Plant Tissue Cult. & Biotech. 26(2): 175-185, 2016 (December)

Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation

2013 ◽  
Vol 35 (5) ◽  
pp. 1675-1689 ◽  
Author(s):  
Muhammad Faizan A. Shukor ◽  
Ismanizan Ismail ◽  
Zamri Zainal ◽  
Normah Mohd Noor
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Polygonum Minus

scholarly journals TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro

2016 ◽  
Vol 5 (2) ◽  
pp. 84 ◽  
Author(s):  
Sri Hutami
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Mass Production ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Physiology

<p>Cell suspension culture could be defined as a<br />process that allows rapidly dividing homogenous suspension<br />of cells to grow in liquid nutrient media. There are two main<br />types of suspension cultures: (1) Batch cultures in which<br />cells are nurtured in a fixed volume of medium until growth<br />ceases and (2) Continuous cultures in which cell growth is<br />maintained by continuous replenishment of sterile nutrient<br />media. Plant cell suspension cultures are mostly used for the<br />biochemical investigation of cell physiology, growth, metabolism,<br />protoplast fusion, transformation and for large scale<br />production of seed by bioreactor and production of secondary<br />metabolites. Contamination is one of the largest problems<br />when dealing with cell cultures. Differences between<br />the products of cell suspension culture and whole plant are<br />frequently observed. These phenomena’s may be resulted<br />from lack of differentiation and organization and cell cultureinduced<br />variation. Utilization of cell suspension culture in<br />Indonesia is still limited, some of them for mass production<br />of plantation seed with bioreactor system and for production<br />of secondary metabolites. The success of this study give the<br />opportunity for mass production of seeds from other plants<br />and also production of secondary metabolites.</p>

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