Evaluation of the Anticancer Properties of Geranyl Isovalerate, an Active Ingredient of Argyreia nervosa Extract in Colorectal Cancer Cells

Chemotherapy is a general treatment procedure for cancer. The diversity in cancer incidence and the failure of therapy due to chemoresistance lead to increased cancer-related deaths. Therefore, new drugs with fewer secondary complications targeting diverse pathways are the need of the hour. Geranyl isovalerate (GIV), one of the active ingredients of ethyl acetate fraction of Argyreia nervosa is routinely used as a food flavoring agent. In this study, we found that GIV also exhibits anticancer activity when tested against the HCT116 cell line. It influenced the viability of the cells in a dose- and time-dependent manner. We examined whether GIV could induce oxidative stress and affect the mitochondrial membrane potential, thereby leading to apoptosis induction. Moreover, GIV could suppress the expression of antiapoptotic genes, such as BCl2 and PARP, and induce the expression of proapoptotic genes, such as Caspase 3 and 9. This is the first study demonstrating the anticancer activity of GIV and providing evidence for its mechanism of action. In conclusion, this study proposes GIV as a potential lead or supplementary molecule in treating and preventing colorectal cancer (CRC). Based on our findings, we conclude that GIV may be a viable lead or supplementary molecule for treating and preventing CRC.

Phytochemical and Spectral analysis of Argoclavine; A potent Psychoactive substance present in the seeds of Argyreia nervosa plant

There are several medicinal plants having various medicinal properties is being widely used in Ayurveda which contain phytoconstituents. These phytoconstituents are beneficial to manage allied symptoms in psychosis andto treat abnormal condition of the mindthereby promoting mental well being. Argyreia nervosa (L.f) the ancient traditional medicinal plant belonging to the convolvulaceae family having prominent, psychedelic and neuro-pharmacological actions on our body. Argyreia nervosa seeds contain hallucinogens including ergot alkaloids such as ergine and Argoclavine and a naturally occurring lysergic acid amide. The current study describes pharmacognostic and phytochemical screening on Seeds of Argyreia nervosa. Argoclavine was extracted from the seeds and then characterised by different spectroscopic techniques like UV, IR, LC-MS. The results of the present study will create a way for the invention of novel herbal medicines for several ailments by using Argyreia nervosa seeds.

Identifikasi Senyawa Terpenoid dan Steroid pada Beberapa Tanaman Menggunakan Pelarut N-Heksan

Hexane is a hydrocarbon compound of alkanes with the chemical formula C6H14. The prefix hex refers to the six carbon atoms found in the hexane and the suffix ana is derived from the word alkane which refers to the single bond connecting the carbon atoms. Identification of terpenoid compounds and steroids has been identified in several plants, namely Red Shoots (Syzygium myrtifolium Walp.), Salam (Syzygium polyanthum), bilaran Tapah (Argyreia nervosa (Burm. F.), Blind (Excoecaria agallocha L.), as well as on the lotion preparations Citrus hystrix DC. This study was conducted to determine the content of terpenoid compounds and steroids found in some parts of plants using n-hexane solvents. Research used is qualitative research with Lieberman Burchard method. Review this article using a method of literature study by searching sources and literature with data form in the form of national journals to international journals about the last 10 years (2012-2021). Identification is carried out on extracts, fractions, isolates and lotion preparations using liquid vacuum chromatography, column chromatography, thin-layer chromatography, and Lieberman-Burchard reagents. The results showed that all of the samples were positive for terpenoid compounds and steroids using n-hexane solvents.

Argyreia nervosa (elephant creeper).

A datasheet on Argyreia nervosa covering, as an economically important tree, its taxonomy, importance, silviculture, distribution, biology and ecology, uses, products and pests.

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples

The consumption of new psychoactive substances (NPSs) has been increasing, and this problem affects several countries worldwide. There is a class of NPSs of natural origin, consisting of plants and fungi, which have a wide range of alkaloids, responsible for causing relaxing, stimulating or hallucinogenic effects. The consumption of some of these substances is prompted by religious beliefs and cultural reasons, making the legislation very variable or even ambiguous. However, the abusive consumption of these substances can present an enormous risk to the health of the individuals, since their metabolism and effects are not yet fully known. Additionally, NPSs are widely spread over the internet, and their appearance is very fast, which requires the development of sophisticated analytical methodologies, capable of detecting these compounds. Thus, the objective of this work is to review the toxicological aspects, traditional use/therapeutic potential and the analytical methods developed in biological matrices in twelve plant specimens (Areca catechu, Argyreia nervosa, Ayahuasca, Catha edulis, Datura stramonium, Lophophora williamsii, Mandragora officinarum, Mitragyna speciosa, Piper methysticum Forst, Psilocybe, Salvia divinorum and Tabernanthe iboga).

Bioinspired triangular ZnO nanoclusters synthesized by Argyreia nervosa nascent leaf extract for the efficient electrochemical determination of vitamin C

This study reports the synthesis of bioinspired triangular ZnO nanoclusters (bT-ZnO NCs) from Argyreia nervosa nascent leaf extract and their use in the agricultural domain for the label-free detection of vitamin C and its antibacterial efficiency.

MEDICINAL PLANT USED FORTHE TREATMENT OF MUSCULAR SPRAIN BY THE TANGKHUL TRIBE OF UKHRUL DISTRICT, MANIPUR, INDIA

Ukhrul district of Manipur is a hilly region predominantly inhabited by the Tangkhul tribe. The Tangkhul people of this hilly region are mainly dependent on the forest and are quiet familiar with local herbs found in the village surroundings and forest areas not only for their food but also provide a major part of the medicine for the treatment of various diseases and ailments especially for the poor people living in the district.Recent ethnomedicinal survey (2016 – 2017) among the Tangkhul community revealed the use of 35 species of angiosperms, covering 34 genera and 25 families which they use to treat muscular sprain.The present study was carried out through structured questionnaires in consultations with the elders and Tangkhul practitioners.Some significant medicinal plants which are used by the Tangkhul tribe for the treatment of muscular sprain are Argyreia nervosa, Cyperus rotundus, Equisetum ramosissinum,Homskioldia sanguine,Mikania cordata, Xylosma longifolia, etc. Plantation of medicinal plant species in home gardens and farm areas has shown its commercial potential and steps for conserving economically significant diverse plants of this hilly region.