scholarly journals Enhanced production of phenolic acids in cell suspension culture of Salvia leriifolia Benth. using growth regulators and sucrose

2018 ◽  
Vol 70 (2) ◽  
pp. 741-750 ◽  
Author(s):  
Masoomeh Modarres ◽  
Sedigheh Esmaeilzadeh Bahabadi ◽  
Mohammad Ehsan Taghavizadeh Yazdi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Enhanced Production

scholarly journals Optimization of cell suspension culture of transformed and untransformed lettuce for the enhanced production of secondary metabolites and their pharmaceutical evaluation

3 Biotech ◽  
2019 ◽  
Vol 9 (9) ◽  
Author(s):  
Hammad Ismail ◽  
Sania Sabahat Kayani ◽  
Sadaf Ilyas Kayani ◽  
Bushra Mirza ◽  
Mohammad Tahir Waheed
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Enhanced Production

Somatic Embryogenesis and Plant Regeneration of Wiregrass (Aristida stricta) and Creeping Bluestem (Schizachyrium scoparium var. stoloniferum)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 479a-479
Author(s):  
Wusi Chen ◽  
Jeffrey G. Norcini ◽  
Robert S. Kalmbacher ◽  
James H. Aldrich
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Embryogenic Callus ◽  
Basal Medium ◽  
Suspension Cultures ◽  
Embryoid Formation ◽  
Semi Solid

Initiation of callus and induction of embryogenesis were achieved from both wiregrass and creeping bluestem. MS basal medium containing coconut milk, sucrose, and 2,4-D were used to initiate callus from young inflorescence of wiregrass and creeping bluestem. The presence of 2,4-D was found to be essential for the induction and early development of embryoids, possibly up to the globular stage. In the case of bluestem, initiation of embryogenic callus required the presence of a low concentration of BA; using only 2,4-D resulted in more non-embryogenic callus. More globular embryos were formed when embryogenic cultures grew rapidly without subculturing, or after being transferred to a hormone-free or a reduced 2,4-D medium. Plant regeneration was carried on a hormone-free MS medium. Initiation of cell suspension and induction of embryoid formation of wiregrass were achieved. However, maintaining cell suspensions seems to have some problems. A majority of the cells were thick-walled, elongated, and non-dividing. No embryos were formed in suspension cultures planted onto solid media. Reinitiation of cell suspension culture of wiregrass is in progress. Initiation of creeping bluestem cell suspension culture was carried out in MS basal medium containing coconut milk, sucrose, and 2,4-D. The maintenance of the cell suspension cultures and induction of embryoid formation were tested under different combinations and concentrations of growth regulators. Suspension cultures were selected and planted onto semi-solid MS basal medium with or without growth regulators. Somatic embryoids formed from suspension culture 3 to 4 weeks after being planted on semi-solid medium. Germination and plant regeneration of somatic embryoid of creeping bluestem are in progress.

scholarly journals Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Sarieh TARIGHOLIZADEH ◽  
Rouhollah MOTAFAKKERAZAD ◽  
Morteza KOSARI-NASAB ◽  
Ali MOVAFEGHI ◽  
Sakineh MOHAMMADI ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Total Phenolics ◽  
Fresh Mass

<p class="042abstractstekst">The impact of combinations of plant growth regulators (PGRs) on callus culture of <em>Satureja sahendica </em>Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l<sup>-1</sup>. The treatment of 2 mg l<sup>-1</sup> 2,4-D + 2 mg l<sup>-1 </sup>Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g<sup>-1</sup>) and flavonoids (24.903 ± 7.016 mg QE g<sup>-1</sup>). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of <em>Satureja sahendica</em> was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.</p>

scholarly journals Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst.

2018 ◽  
Vol 28 (2) ◽  
pp. 191-199 ◽  
Author(s):  
AV Kharde ◽  
SV Kore ◽  
MB Khetmalas
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Bacopa Monnieri ◽  
Naphthalene Acetic Acid

Cell suspension culture is used for the optimization of plant growth regulators used for the elicitation of bacoside content of Bacopa monnieri (L.) Wettst. Callus induction was carried out on MS supplemented with 2, 4-D (0.5 mg/l) by using leaf explants. For suspension culture MS liquid medium was supplemented with constant α-naphthalene acetic acid (0.5 mg/l) and casein hydrolysate (1 g/l) as well as the varied concentrations of 6-benzyleadenine, kinetin, thidiazuaron and chitosan. It was observed that bacoside production was increased by 6- benzyleadenine (0.5 mg/l), Kn (0.5 mg/l) and thidiazuaron (0.25 mg/l) was 3.07, 3.79 and 3.97 mg/g, respectively. This indicates that lower concentration of growth regulator favor the bacoside enhancement under in vitro conditions, while chitosan affects the bacoside production in suspension culture. In this study thidiazuaron at 0.25 mg/l induces maximum elicitation of bacoside content i.e. 3.97 mg/g. Plant Tissue Cult. & Biotech. 28(2): 191-199, 2018 (December)

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