Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

2019 ◽  
Vol 136 (3) ◽  
pp. 489-498 ◽  
Author(s):  
Marzieh Taghizadeh ◽  
Fatemeh Nasibi ◽  
Khosrow Manouchehri Kalantari ◽  
Faezeh Ghanati
Keyword(s):  
Magnetic Field ◽  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Static Magnetic Field ◽  
Cell Suspension Culture ◽  
Magnetite Nanoparticles

scholarly journals Lupeol acetate production and antioxidant activity of a cell suspension culture from Cnidoscolus chayamansa leaves

2019 ◽  
Vol 125 ◽  
pp. 30-38 ◽  
Author(s):  
M.Z. Pérez-González ◽  
A. Nieto-Trujillo ◽  
G.A. Gutiérrez-Rebolledo ◽  
I. García-Martínez ◽  
M.E. Estrada-Zúñiga ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Acetate Production ◽  
A Cell ◽  
Lupeol Acetate ◽  
Cnidoscolus Chayamansa

Effects of different factors on friable callus induction and establishment of cell suspension culture of Hovenia dulcis (Rhamnaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Ivan Gonçalves Ribeiro ◽  
Tatiana Carvalho de Castro ◽  
Marsen Garcia Pinto Coelho ◽  
Norma Albarello
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Friable Callus ◽  
Weight Analysis ◽  
Hovenia Dulcis

Abstract Medicinal plants are an important therapeutic option for a large share of the world’s population. To establish an in vitro culture system for the production of secondary metabolites from Hovenia dulcis, we studied the effect of auxins, cytokinins, absence of light, and silver nitrate on the development of friable callus. Callus cultures were established for the first time and used to obtain cell suspension cultures. Supplementation with KIN (Kinetin) produced calli with both compact and friable areas, while the addition of TDZ (Thidiazuron) only produced compact callus. The maintenance of cultures in the dark induced a slight enhancement on friability when the auxin PIC (Picloram) was present in the culture medium. The addition of silver nitrate promoted the formation of friable calli. Dry weight analysis showed no significant differences in biomass growth, and, therefore, 2.0 mg.L-1 was considered the most suitable treatment. The presence of silver nitrate was not required for the establishment of cell suspension cultures. Dry weight analysis of cell suspensions showed higher biomass production in the absence of silver nitrate. PIC promoted 100% of cell suspension culture formation in the absence of silver nitrate, and higher biomass production was observed with the lowest concentration (0.625 mg.L-1). No morphological differences were observed among the different concentrations of PIC. Phytochemical screening showed the presence of saponins, flavonoids, flavonols and catechins in the extracts obtained from H. dulcis calli. These results show that the cell cultures herein established are potential sources for the production of H. dulcis secondary metabolites of medicinal interest.

scholarly journals Sink ? Source System of In vitro Suspension Culture of Celastrus paniculatus under Regulation of Monochromatic Lights

2016 ◽  
Vol 26 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Vandita Billore ◽  
Lalit Khatediya ◽  
Monica Jain
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Cell Mass ◽  
Scale Up ◽  
Light Conditions ◽  
Yellow Light ◽  
Celastrus Paniculatus

Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high?value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids (91.69 ?g/1750 ml) and phenols (70.59 ?g/1750 ml) was obtained during 30 days of culture under yellow light conditions. The production was remarkably enhanced by 100?folds (5.29 to 48.21 ?g; Fig. 2) for alkaloids and 70?folds (1.73 to 46.33 ?g; Fig. 3) for phenols, from zero days to 30?day?old culture phase. Hence, strategic implementation of monochromatic lights holds great promises for controlled commercial production of myriads of valuable secondary metabolites from plant cell cultures of important medicinal plants. Present work contributes to the first reports where continuous, enhanced, multifold yield of important secondary metabolites were obtained from cell suspension culture of Celasrus paniculatus an endangered medicinal plant.Plant Tissue Cult. & Biotech. 26(2): 175-185, 2016 (December)

scholarly journals Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

2017 ◽  
Vol 45 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Izabela WEREMCZUK-JEŻYNA ◽  
Izabela GRZEGORCZYK-KAROLAK ◽  
Barbara FRYDRYCH ◽  
Katarzyna HNATUSZKO-KONKA ◽  
Aneta GERSZBERG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Total Phenolic Content ◽  
Rosmarinic Acid ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic

Dracocephalum moldavica L. (Lamiaceae) is known for its medicinal properties, however greater yields can potentially be achieved by in vitro cultivation. A cell suspension culture of D. moldavica L. (Lamiaceae) derived from root-derived callus was established in liquid MS medium supplemented with 2,4-D 0.5 mg/l and BAP 0.2 mg/l. The biomass and rosmarinic acid (RA) content were analyzed during the 15-day growth cycle of the culture. The highest fresh and dry weight (14.29 g/flask and 1.14 g/flask, respectively) and RA level (27.2 mg/g DW) were reached at day 12 of culture. Methanolic extracts of the culture were assayed for total phenolic content using the Folin-Ciocalteau method, and antioxidant activities using three in vitro tests: ABTS radical scavenging, ferric ion reduction (FRAP) and lipid peroxidation (LPO). RA content and antioxidant potential were found to be higher in cell suspension culture than in root-derived callus. The cell suspension culture also exhibited higher concentrations of RA and ABTS radical scavenging activity than those of the aerial parts of six-month-old field-grown plants of D. moldavica. The overall results show a significant correlation between antioxidant activity, total phenolic content and RA content of the examined extracts. The study presents for the first time the use of cell cultures of D. moldavica for production of therapeutically-valuable metabolites. Our results suggest that the obtained culture could be considered as a potential source of rosmarinic acid, a compound known for its strong antioxidant activity.

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