Development and validation of a culture-based method suitable for monitoring environmental survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in developing countries

2011 ◽  
Vol 61 (4) ◽  
pp. 809-817 ◽  
Author(s):  
Duncan Ongeng ◽  
Charles Muyanja ◽  
Jaak Ryckeboer ◽  
Annemie Helena Geeraerd ◽  
Dirk Springael
Keyword(s):  
Escherichia Coli ◽  
Developing Countries ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Escherichia Coli O157 ◽  
Environmental Survival ◽  
Serovar Typhimurium ◽  
Development And Validation

Detection of Escherichia coli O157, Salmonella enterica Serovar Typhimurium, and Staphylococcal Enterotoxin B in a Single Sample Using Enzymatic Bio-Nanotransduction

2007 ◽  
Vol 70 (4) ◽  
pp. 841-850 ◽  
Author(s):  
JOSH R. BRANEN ◽  
MARTHA J. HASS ◽  
ERIN R. DOUTHIT ◽  
WUSI C. MAKI ◽  
A. LARRY BRANEN
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Staphylococcal Enterotoxin B ◽  
Escherichia Coli O157 ◽  
Dna Templates ◽  
E Coli ◽  
Serovar Typhimurium ◽  
Biological Recognition ◽  
Enterotoxin B

Enzymatic bio-nanotransduction is a biological detection scheme based on the production of nucleic acid nano-signals (RNA) in response to specific biological recognition events. In this study, we applied an enzymatic bio-nanotransduction system to the detection of important food-related pathogens and a toxin. Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and staphylococcal enterotoxin B (SEB) were chosen because of the implications of these targets to food safety. Primary antibodies to each of the targets were used to functionalize magnetic beads and produce biological recognition elements (antibodies) conjugated to nano-signal–producing DNA templates. Immunomagnetic capture that was followed by in vitro transcription of DNA templates bound to target molecules produced RNA nano-signals specific for every target in the sample. Discrimination of RNA nano-signals with a standard enzyme-linked oligonucleotide fluorescence assay provided a correlation between nano-signal profiles and target concentrations. The estimated limit of detection was 2.4 × 103 CFU/ml for E. coli O157:H7, 1.9 × 104 CFU/ml for S. enterica serovar Typhimurium, and 0.11 ng/ml for SEB with multianalyte detection in buffer. Low levels of one target were also detected in the presence of interference from high levels of the other targets. Finally, targets were detected in milk, and detection was improved for E. coli O157 by heat treatment of the milk.

Antibody-based Detection of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium Grown in Low-shear Modeled Microgravity

2007 ◽  
Vol 20 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Cynthia Nyquist-Battie ◽  
Laura Freeman ◽  
Kristen Leckband ◽  
Stephanie Martinez ◽  
Ariel Ansley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Escherichia Coli O157 ◽  
Modeled Microgravity ◽  
Serovar Typhimurium ◽  
Low Shear

scholarly journals Genome Sequence of Bacteriophage GG32, Which Can Infect both Salmonella enterica Serovar Typhimurium and Escherichia coli O157:H7

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Su-Jin Chae ◽  
Taesoo Kwon ◽  
Sunjin Lee ◽  
Yeon Ho Kang ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Control Agent ◽  
Republic Of Korea ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Serovar Typhimurium ◽  
The Republic

We report here a new virulent Salmonella enterica serovar Typhimurium ( S . Typhimurium) bacteriophage, GG32, which was isolated from the Guem River in the Republic of Korea. The strain can infect both S . Typhimurium and Escherichia coli ( E. coli ) O157:H7 and may be a good candidate for a bio-control agent.

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